Proc (NM_008934) Mouse Untagged Clone

CAT#: MC227922

Proc (untagged) - Mouse protein C (Proc), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Proc
• Synonyms: P; PC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227922 representing NM_008934
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTACCTGGACGAAATTGCAGTTTCTCCTTGGCCCACGCCTGTGTCAGCAGCTCCAGGATGTGGC
  AATTCAGAGTCTTCCTGCTGCTCATGTCCACCTGGGGAATATCTAGCATACCGGCCCATCCTGACCCAGT
  GTTCTCCAGCAGCGAGCATGCCCACCAGGTGCTTCGGGTCAGACGTGCCAACAGCTTCCTGGAAGAGATG
  CGGCCAGGCAGCCTGGAACGGGAGTGTATGGAGGAGATCTGTGACTTCGAGGAGGCCCAGGAGATTTTCC
  AAAATGTGGAAGACACACTGGCCTTCTGGATCAAGTACTTTGACGGTGACCAGTGCTCGGCTCCACCCTT
  GGACCACCAGTGCGACAGCCCATGCTGCGGGCATGGCACTTGCATCGACGGCATAGGCAGCTTCAGCTGC
  AGCTGCGATAAGGGCTGGGAGGGCAAGTTCTGTCAGCAGGAGTTGCGCTTCCAGGACTGTCGGGTGAACA
  ATGGCGGCTGCTTGCACTACTGCCTGGAGGAGAGCAATGGGCGGCGCTGCGCTTGTGCCCCGGGCTATGA
  GCTGGCAGACGACCACATGCGCTGCAAGTCCACTGTGAATTTTCCATGTGGGAAACTGGGGAGGTGGATA
  GAGAAGAAACGCAAGATCCTCAAACGAGACACAGACTTAGAAGATGAACTGGAACCAGATCCAAGGATAG
  TCAACGGAACGCTGACGAAGCAGGGTGACAGTCCTTGGCAGGCAATCCTTCTGGACTCCAAGAAGAAGCT
  GGCCTGCGGAGGGGTGCTCATCCACACTTCCTGGGTGCTGACGGCAGCCCACTGCGTGGAGGGCACCAAG
  AAGCTTACCGTGAGGCTTGGTGAGTATGATCTGCGACGCAGGGACCACTGGGAGCTGGACCTGGACATCA
  AGGAGATCCTCGTCCACCCTAACTACACCCGGAGCAGCAGTGACAACGACATTGCTCTGCTCCGCCTAGC
  CCAGCCAGCCACTCTCTCCAAAACCATAGTGCCCATCTGCCTGCCGAACAATGGGCTGGCGCAGGAGCTC
  ACTCAGGCTGGCCAGGAGACAGTGGTGACAGGCTGGGGCTATCAAAGCGACAGAATCAAGGATGGCAGAA
  GGAACCGCACCTTCATCCTCACCTTCATCCGCATCCCTTTGGTTGCTCGAAATGAGTGCGTGGAGGTCAT
  GAAGAATGTGGTCTCGGAGAACATGCTGTGTGCAGGCATCATTGGGGACACGAGAGACGCCTGTGATGGT
  GACAGTGGGGGGCCCATGGTGGTCTTCTTTCGGGGTACCTGGTTCCTGGTGGGCCTGGTGAGCTGGGGTG
  AGGGCTGTGGGCACACCAACAACTATGGCATCTACACCAAAGTGGGAAGCTACCTCAAATGGATTCACAG
  TTACATTGGGGAAAAGGGTGTCTCCCTTAAGAGCCAGAAGCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008934
• Insert Size: 1446 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008934.4, NP_032960.3
• RefSeq Size: 1584 bp
• RefSeq ORF: 1446 bp
• Locus ID: 19123
• Cytogenetics: 18 B1
• Gene Summary: This gene encodes the vitamin K-dependent protein C, which plays a vital role in the anticoagulation pathway. The encoded protein undergoes proteolytic processing including activation by thrombin-thrombomodulin complex to form the anticoagulant serine protease that degrades activated coagulation factors. A complete lack of the encoded protein in mice results in severe perinatal consumptive coagulopathy in the brain and liver, resulting in death within 24 hours after birth. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) encodes the longest isoform (1). This isoform (1) may undergo proteolytic processing similar to isoform 2.