Lef1 (NM_001276403) Mouse Untagged Clone

CAT#: MC227075

Lef1 (untagged) - Mouse lymphoid enhancer binding factor 1 (Lef1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lef1
• Synonyms: 3000002B05; AI451430; Lef-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227075 representing NM_001276403
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGGTGGTCAGACAAGCCCCGTCCTCTCAGGAGCCCTACCACGACAAGGCCAGAGAACACCCTG
  ATGAAGGAAAGCATCCAGACGGAGGCCTGTACAACAAGGGACCCTCCTACTCCAGTTACTCTGGCTACAT
  AATGATGCCCAATATGAACAGCGACCCGTACATGTCAAATGGGTCCCTTTCTCCACCCATCCCGAGGACA
  TCAAATAAAGTGCCCGTGGTGCAGCCCTCTCACGCGGTCCACCCGCTCACCCCCCTCATCACCTACAGCG
  ACGAGCACTTTTCTCCGGGATCCCACCCGTCACACATCCCGTCAGATGTCAACTCCAAGCAAGGCATGTC
  CAGACACCCTCCAGCTCCTGAAATCCCCACCTTCTACCCCCTGTCTCCGGGCGGCGTTGGACAGATCACC
  CCACCCATTGGCTGGCAAGGTCAGCCTGTTTATCCCATCACGGGTGGATTCAGGCAACCCTACCCATCCT
  CACTGTCAGGCGACACTTCCATGTCCAGGTTTTCCCATCATATGATTCCTGGTCCCCCTGGCCCCCACAC
  AACTGGCATCCCTCATCCAGCTATTGTAACACCTCAGGTCAAACAGGAGCACCCCCACACGGACAGTGAC
  CTAATGCACGTGAAGCCTCAACACGAACAGAGAAAGGAGCAGGAGCCCAAAAGACCTCATATTAAGAAGC
  CTCTGAATGCTTTCATGTTATATATGAAAGAAATGAGAGCGAATGTCGTAGCTGAGTGCACGCTAAAGGA
  GAGTGCAGCTATCAACCAGATCCTGGGCAGAAGATGGCACGCCCTCTCCCGGGAAGAGCAGGCCAAATAC
  TATGAACTAGCACGGAAAGAGAGACAGCTACACATGCAGCTTTATCCAGGCTGGTCAGCGCGAGACAATT
  ATGGCAAGAAGAAGAAGAGGAAGAGAGAGAAGCTACAGGAGTCGACTTCAGGTACAGGTCCCAGAATGAC
  AGCTGCCTACATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276403
• Insert Size: 996 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276403.1, NP_001263332.1
• RefSeq Size: 2305 bp
• RefSeq ORF: 996 bp
• Locus ID: 16842
• Cytogenetics: 3 60.78 cM
• Gene Summary: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate 5' exon and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) is shorter and has a distinct N-terminus, compared to isoform a.