Adra1a (NM_001271761) Mouse Untagged Clone

CAT#: MC226867

Adra1a (untagged) - Mouse adrenergic receptor, alpha 1a (Adra1a), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adra1a
• Synonyms: Adr; Adra1c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226867 representing NM_001271761
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCTTCTTTCTGAAAATGCTTCTGAAGGCTCCAACTGCACCCACCCGCCAGCACAGGTGAACATTT
  CTAAGGCCATTCTACTTGGGGTGATCTTGGGGGGCCTCATCATTTTCGGGGTCTTGGGGAATATTTTAGT
  GATCCTCTCGGTGGCCTGTCATCGGCATCTGCACTCGGTGACTCACTACTACATTGTCAACCTGGCTGTG
  GCAGACCTCCTCCTCACCTCCACCGTGCTGCCCTTCTCTGCCATCTTTGAGATCCTGGGCTACTGGGCCT
  TTGGCAGGGTGTTCTGCAACATCTGGGCGGCGGTGGACGTCTTATGCTGCACAGCGTCCATCATGGGCCT
  CTGCATCATCTCCATCGACCGATACATTGGTGTGAGCTACCCGCTGCGCTACCCCACCATTGTCACCCAG
  AGGAGGGGCGTCAGGGCTCTGCTCTGCGTCTGGGCGCTTTCCTTGGTCATCTCCATCGGACCCCTGTTCG
  GCTGGAGGCAGCAGGCTCCGGAGGATGAGACCATCTGCCAAATCAATGAGGAGCCAGGATACGTGCTGTT
  CTCAGCGCTGGGCTCTTTCTACGTGCCACTGACCATCATCCTGGTTATGTACTGTCGAGTCTACGTGGTA
  GCCAAGAGAGAAAGCCGAGGCCTCAAGTCCGGCCTCAAGACCGACAAGTCAGACTCAGAGCAAGTGACGC
  TCCGTATCCACCGTAAAAATGTCCCTGCAGAAGGCAGCGGAGTAAGCAGTGCCAAGAATAAGACTCACTT
  CTCCGTGAGGCTGCTCAAGTTTTCCCGAGAGAAGAAAGCCGCCAAGACGCTGGGCATTGTGGTGGGATGC
  TTCGTCCTCTGCTGGCTGCCATTCTTCCTCGTGATGCCCATTGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271761
• Insert Size: 888 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271761.1, NP_001258690.1
• RefSeq Size: 2417 bp
• RefSeq ORF: 888 bp
• Locus ID: 11549
• Cytogenetics: 14 D1
• Gene Summary: This gene encodes one of several multipass transmembrane proteins that function as G protein-coupled receptors. The encoded protein binds to epinephrine and norepinephrine to mediate signaling in cells of the cardiac, nervous, and other organ systems. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (4) differs in the 5' UTR, lacks two exons, and includes an alternate 3' terminal exon, compared to variant 1. The encoded isoform (3) has a shorter C-terminus, compared to isoform 1.