Cdc42bpa (NM_001033285) Mouse Untagged Clone

CAT#: MC224883

Cdc42bpa (untagged) - Mouse CDC42 binding protein kinase alpha (Cdc42bpa), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdc42bpa
• Synonyms: A930014J19Rik; DMPK-like
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224883 representing NM_001033285
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGAGAAGTGCGTTTGAGGCAGTTGGAGCAGTTTATTTTAGATGGGCCAGCTCAGACCAATGGGC
  AGTGCTTCAGTGTGGAGACGCTACTGGACATCCTCATCTGTCTTTACGATGAATGCAATAATTCTCCATT
  GAGAAGAGAGAAGAACATTCTTGAATATCTAGAATGGGCTAAACCATTTACTTCTAAAGTGAAGCAAATG
  CGATTACATAGAGAAGACTTTGAAATATTAAAGGTGATTGGTCGAGGAGCTTTTGGGGAGGTTGCTGTAG
  TTAAACTAAAAAATGCAGATAAAGTATTCGCCATGAAAATTCTGAACAAGTGGGAAATGCTGAAAAGAGC
  TGAGACAGCGTGCTTTCGTGAAGAGAGGGATGTGTTGGTAAATGGAGACAGTAAGTGGATAACCACTCTG
  CACTATGCTTTCCAGGATGACAATAACTTATACCTGGTTATGGATTATTATGTTGGTGGGGATTTACTTA
  CTCTGCTCAGCAAATTTGAAGATAGATTGCCTGAAGAGATGGCTCGGTTTTACTTGGCTGAGATGGTGAT
  AGCCATTGACTCAGTTCACCAGCTCCACTATGTTCACAGAGACATCAAACCTGACAATATATTGATGGAT
  ATGAATGGACATATTCGTTTAGCAGATTTTGGTTCTTGTCTGAAGCTGATGGAAGATGGAACGGTCCAGT
  CCTCGGTGGCAGTTGGAACTCCAGATTATATTTCTCCGGAAATCCTTCAGGCTATGGAAGACGGAAAAGG
  CAGATATGGACCAGAGTGTGACTGGTGGTCCTTGGGGGTCTGTATGTATGAGATGCTTTACGGAGAAACA
  CCATTCTATGCAGAGTCTCTGGTGGAGACATATGGAAAAATTATGAATCACAAAGAGCGGTTTCAGTTTC
  CAGCCCAAGTGACAGACGTGTCCGAAAATGCTAAAGATCTTATTCGAAGACTCATTTGTAGTAGAGAACA
  TCGACTTGGCCAAAATGGAATAGAAGACTTTAAGAAGCATCCATTCTTCAGTGGAATTGATTGGGATAAC
  ATTCGGAATTGTGAAGCACCTTACATCCCAGAAGTTAGTAGCCCGACAGATACGTCAAATTTTGATGTGG
  ATGACGACTGTTTAAAAAATTCTGAAACAATGCCCCCACCAACACATACTGCATTTTCTGGCCATCATCT
  ACCATTTGTTGGTTTTACATATACTAGTAGCTGTGTTCTTTCTGATCGGAGCTGTTTAAGAGTCACAGCA
  GGCCCCACCTCATTGGACCTTGATGTCAGTGTCCAGAGGACTCTAGACAATAACTTAGCAACTGAGGCTT
  ATGAAAGGAGAATTAAGCGGCTTGAGCAGGAAAAGCTTGAACTGACTAGAAAACTTCAAGAGTCAACACA
  GACTGTTCAAGCTCTGCAATATTCAACTGTTGATGGTCCACTAACGGCAAGCAAAGATTTAGAAATTAAA
  AGTCTGAAAGAAGAGATTGAAAAACTAAGGAAACAAGTAGCAGAAGTGAATCATTTGGAACAACAACTTG
  AAGAAGCTAATTCTGTGAGACGAGAACTAGATGATGCTTTCAGGCAAATCAAAGCTTCTGAAAAACAAAT
  CAAAACTTTGCAGCAGGAAAGAGAAGAACTAAATAAGGAACTAGTCCAGGCTAGTGAGCGATTAAAAAAC
  CAATCCAAAGAGCTGAAAGATGCACACTGTCAGAGGAAACTGGCCATGCAGGAATTCATGGAGATCAATG
  AGCGACTAACAGAATTGCACACCCAAAAGCAGAAACTTGCTCGCCATGTCCGAGATAAGGAAGAAGAGGT
  GGACCTGGTGATGCAAAAAGCTGAGAGCTTAAGGCAGGAGCTGCGCAGAGCAGAAAGAGCCAAGAAAGAG
  CTGGAAGTTCACACCGAAGCTCTAATTGCTGAAGCATCAAAGGACAAGAAGCTCCGAGAGCAGAGCGAAC
  ATTATTCTAAGCAACTGGAAAACGAGTTGGAGGGCCTGAAGCAAAAACAAATTAGTTACTCACCAGGAAT
  ATGCAGCATAGAACATCAGCAAGAGATAACTAAACTGAAAACTGACTTGGAAAAGAAAAGTATCTTTTAT
  GAAGAAGAAATTTCTAAAAGAGAAGGAATTCACGCAAGTGAGATTAAAAACCTGAAGAAGGAGCTGCATG
  ACTCAGAAGGCCAGCAGCTTGCTCTCAACAAAGAGATTCTGGTTTTAAAAGACAAATTGGAAAAAACCAG
  GAGAGAAAGTCAAAGTGAAAGGGAAGAATTTGAAAATGAGTTCAAACAACAATATGAACGGGAAAAAGTA
  TTATTAACTGAAGAGAATAAAAAGTTAACGAGTGAACTCGATAAGCTGACTTCACTGTATGAGAGCTTAA
  GTTTGCGCAACCAGCACTTAGAAGAAGAAGTCAAAGATCTAGCAGATAAGAAGGAATCAGTTGCCCACTG
  GGAAGCCCAAATCACAGAGATAATTCAGTGGGTGAGCGATGAGAAGGATGCACGAGGCTATCTTCAGGCC
  TTAGCTTCTAAAATGACTGAAGAGTTGGAAGCATTAAGAAATTCCAGCTTGGGGACACGAGCAACAGATA
  TGCCCTGGAAAATGCGTCGTTTTGCAAAACTGGACATGTCGGCTAGACTAGAGTTGCAGTCTGCTCTGGA
  TGCAGAAATAAGAGCTAAGCAGGCCATCCAGGAAGAGCTGAATAAAGTCAAAGCATCTAACATCTTAACA
  GAATGTAAACTGAAAGATTCAGAGAAGAAGAACTTGGAACTGTTGTCCGAAATCGAGCAGCTGATAAAGG
  ACACTGAAGAGCTTAGATCTGAAAAGGGTATAGAGCACCAAGACTCACAGCATTCTTTCTTGGCATTTTT
  GAATACGCCTACCGATGCTCTGGACCAATTTGAACGCTCTCCGTCCTGTACTCCAGCTAGCAAAGGCAGA
  CGTATTGCAGACTGTGCTCCACTTCCAGCTCACACACCAACCTTAAGGAAAAAGGGATGCCCGGCTTCAA
  CTGGCTTTCCACCTAAGCGCAAGACTCATCAATTTTTCGTGAAGTCTTTCACTGCCCCTACCAAGTGTCA
  CCAGTGCACCTCCTTGATGGTCGGTTTGATAAGACAGGGCTGTTCCTGTGAAGTCTGTGGGTTCTCATGT
  CATATAACTTGTGTGAACAAAGCCCCGACGGTTTGCCCAGTTCCTCCTGAGCAAACTAAAGGTCCACTGG
  GCATAGACCCCCAGAAGGGAGTTGGGACGGCGTATGAGGGTCATGTCAGGATCCCTAAGCCGGCGGGAGT
  GAAGAAAGGGTGGCAGAGAGCACTGGCTGTGGTCTGCGACTTCAAACTGTTTCTTTATGATATCGCAGAG
  GGAAAAGCATCGCAGCCCACCTCCGTCATTAGTCAAGTGATTGACATGAGAGATGAAGAATTTTCTGTGA
  GTTCAGTCTTAGCTTCTGATGTTATTCACGCAAGTCGAAAAGATATCCCCTGTATATTCAGGGTCACAGC
  TTCCCAGCTCTCAGCACCTAGTAACAAATGTTCCATCCTGATGCTAGCAGACAGCGAGAATGAGAGGAGT
  AAGTGGGTGGGAGTGCTGAGTGAACTCCACAAGATTCTGAAGAAAAACAAGTTCAGAGACCGCTCAGTGT
  ATGTCCCCAAGGAGGCGTACGACAGCACGCTACCCCTCATTAAGACAACGCAGGCCGCTGCAATCATAGA
  TCATGAACGGATAGCTTTGGGAAATGAAGAAGGATTATTTGTTGTGCATGTCACCAAAGATGAGATTGTT
  AGAGTTGGTGATAATAAGAAAATCCATCAGATCGAACTCATTCCAAGTGACCAGCTTGTCGCTGTGATCT
  CAGGCCGAAATCGCCACGTCCGACTTTTTCCTATGTCAGCCTTGGATGGGCGAGAGACGGATTTTTATAA
  ACTGGCAGAGACTAAAGGGTGTCAGACCATAGCTGCTGGGAAAGTGCGCCACGGAGCTCTGTCCTGCCTG
  TGTGTGGCCATGAAGAGACAGGTCCTCTGCTATGAACTGTTCCAAAGCAAGACCAGGCACAGGAAGTTTA
  AGGAGATTCAAGTCCCATGTAATGTCCAGTGGATGGCCATCTTCAGTGAGCATCTCTGTGTGGGATTCCA
  GTCAGGATTTCTCAGATACCCCCTGAATGGAGAAGGAGGTCCATGCAATATGCTCCATTCAAATGACCAT
  ACACTGTCATTCATTTCACACCAACCAATGGACGCTCTCTGTGCAGTTGAGATCTCCAACAAAGAGTACT
  TGCTGTGTTTTAACAGCATTGGGATATACACAGACTGCCAGGGCCGAAGATCCAGACAGCAGGAACTGAT
  GTGGCCAGCAAATCCTTCCTCCTGTTGTTACAATGCTCCATATCTCTCCGTGTATAGTGAAAACGCAGTT
  GATATCTTTGATGTAAACTCCATGGAATGGATACAGACTCTTCCTCTCAAAAAGGTCCGACCTTTAAACA
  CTGAAGGATCATTAAATCTCTTAGGATTGGAGACCATTAGATTAATATATTTCAAAAATAAGATGGCAGA
  AGGGGATGAACTTGTGGTTCCTGAAACATCAGATAATAGTCGGAAACAAATGGTTAGAAATATCAACAAC
  AAACGGCGTTACTCCTTCCGAGTCCCTGAAGAAGAGAGGATGCAACAGAGGAGGGAGATGCTACGAGATC
  CAGAAATGAGAAATAAATTAATTTCTAACCCAACTAATTTTAACCACATAGCACACATGGGTCCTGGAGA
  TGGAATACAGATCCTAAAAGACCTGCCCATGAACCCACGGCCTCAGGAAAGCCGGACAGTATTCAGTGGC
  TCCGTCAGTATTCCCTCCATCACCAAGTCCCGCCCTGAGCCAGGCCGCTCCATGAGTGCCAGCAGCGGCC
  TGTCTGCACGGTCGTCAGCCCAGAATGGCAGTGCACTAAAGAGGGAATTCTCTGGAGGAAGCTATAACAC
  AAAGCGACAGCCCATGCCCTCTCCCTCGGAGGGCTCTTTGTCATCAGGAGGCATGGACCAAGGAAGTGAC
  GCCCCTGCGAGGGACTATGATGGAGAGGACTCTGATTCTCCAAGGCATTCCACAGCTTCCAACAGCTCTA
  ACCTGAGCAGCCCCCCAAGTCCCATTTCACCCCAAAAGACCAAGAGCCTCTCCCTGGAGAGCACAGACCG
  CGGGAGCTGGGATCCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033285
• Insert Size: 5199 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033285.1, NP_001028457.1
• RefSeq Size: 8656 bp
• RefSeq ORF: 5199 bp
• Locus ID: 226751
• Cytogenetics: 1 H4
• Gene Summary: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LRP35A, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A and LIMK2. May play a role in TFRC-mediated iron uptake (By similarity). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (PubMed:25107909).[UniProtKB/Swiss-Prot Function]