Med1 (NM_013634) Mouse Untagged Clone
CAT#: MC224683
Med1 (untagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 1, (10ug)
"NM_013634" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Med1 |
Synonyms | AI480703; CRSP210; DRIP205; l11Jus15; PBP; Pparbp; TRAP220 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC224683 representing NM_013634
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCCCTCCTGGAACGGCTCCATGCAAAATTTAACCAGAACAGACCTTGGAGTGAAACCATTAAGC TTGTGCGTCAAGTAATGGAGAAGAGGGTCGTAATGAGTTCTGGAGGGCATCAGCATTTGGTCAGCTGTTT GGAGACATTGCAGAAGGCTCTCAAAGTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCTATAGCC AGACAGAATGGACTGGGCTCTCACCTCAGTGCCAGTGGCACTGAGTGTTACATCACGTCAGATATGTTCT ATGTGGAAGTGCAGTTAGATCCTGCAGGACAGCTTTGTGATGTCAAAGTGGCTCACCATGGGGAGAATCC TGTGAGCTGTCCAGAGCTTGTACAGCAGTTAAGGGAAAAGAATTTTGAGGAATTTTCCAAGCATCTTAAG GGTCTTGTTAATCTGTATAATCTCCCAGGGGACAACAAACTGAAGACTAAAATGTATCTGGCTCTCCAAT CCTTAGAACAGGACCTTTCTAAAATGGCTATTATGTACTGGAAGGCAACCAACGCCGCTCCCTTGGATAA GATTCTTCATGGAAGTGTTGGTTATCTCACCCCGCGGAGTGGGGGTCATTTAATGAATATGAAATACTAT GCCTCTCCATCTGACCTGCTGGATGATAAGACTGCCTCTCCTATCATTTTGCATGAAAAGAATGTTCCTC GGTCTTTGGGAATGAATGCCTCAGTGACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCC ATTAATTATGGGGTCACACCCAGCTGACAACAAATGGACCCCTTCTTTCTCCGCAGTCACTAGTGCCAAC AGTGTTGATCTTCCTGCGTGTTTCTTCTTGAAATTTCCCCAGCCAATTCCAGTATCTAAAGCATTTGTTC AGAAACTGCAAAATTGCACAGGAATCCCGTTGTTTGAGACTCCGCCCACTTACCTGCCCCTGTATGAACT CATCACTCAGTTTGAGCTGTCAAAGGATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCT GCTCTTCCAGGTCAGCAGCACTGCTATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGTCAGAGCCTGC AGGGAACACTGGTCAGCAAAATCACCTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAG ACACCAAGTGGCCTATAACACTCTAATTGGAAGCTGTGTCAAAAGAACTATTTTAAAAGAAGATTCTCCT GGGCTCCTCCAGTTTGAAGTGTGTCCTCTCTCAGAATCTCGCTTCAGTGTATCTTTTCAGCACCCTGTGA ATGACTCCCTTGTGTGTGTGGTGATGGATGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGG GCTGTCAGATGCACTAATCTGTACAGACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATTCCT GTGACGATGAGGGCTATTCGGAGGAAGGCTGAAACCATACAGGCTGACACCCCAGCACTGTCTCTCATTG CAGAGACAGTTGAAGACATGGTGAAAAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCAC AGGCAACAACCCAATGAGTGGTACCACTACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTG TTTAATATGAGCATGAGCATCAAAGATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGT CTCAGAACCCAATTCTTACCAGTTTGTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCC GACCCCTCCTCATCACACGCCGCCACCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTC ATGAACCTTCTTAAAGATAACCCTGCCCAGGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGC AGAACTCCTCTTCCGGATCACCCCGGATGGAAATGTGCTCGGGGAGCAACAAGGCCAAGAAGAAGAAGTC GTCAAGAGTCCCACCTGACAAACCCAAGCACCAGACTGAAGACGATTTCCAGAGGGAGCTCTTTTCCATG GATGTCGACTCACAGAACCCTATGTTTGACGTCAGCATGACCGCTGACGCGCTGGATACACCTCATATCA CCCCAGCTCCAAGCCAGTGTAGCACTCCCCCAGCAACGTACCCACAGCCAGTGTCTCACCCCCAGCCCAG TATTCAGAGGATGGTCCGACTGTCCAGTTCAGACAGCATTGGCCCAGATGTAACTGATATTCTTTCAGAT ATTGCCGAAGAAGCTTCAAAGCTTCCCAGCACGAGTGATGACTGCCCACCAATTGGCACCCCTGTTCGAG ATTCCTCAAGTTCTGGGCATTCTCAGAGTGCCCTCTTTGATTCTGATGTCTTTCAAACTAATAATAATGA AAATCCATACACTGATCCAGCTGACCTTATTGCAGATGCTGCTGGAAGCCCCAATAGTGATTCTCCTACC AATCATTTTTTCCCTGATGGAGTAGATTTCAATCCTGATTTGTTGAACAGCCAAAGCCAAAGTGGTTTTG GAGAGGAGTATTTTGATGAAAGTAGTCAGAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGC ATTAAATACATTGGGGATGCCAATGCTTGGAGGTGACAATGGGGAGCCAAAATTTAAGGGCAGCAGCCAG GCTGACACGGTGGACTTCAGTATTATATCAGTAGCCGGTAAGGCTTTGGGTGCTGCAGATCTGATGGAGC ACCACAGTGGGAGTCAGAGTCCTTTACTGACCACTGGAGAATTAGGGAAAGAAAAAACTCAAAAGAGGGT GAAGGAAGGCAACGGCACAGGTGCTAGCAGTGGATCAGGTCCAGGGTCAGACAGCAAGCCAGGCAAGCGC AGCCGCACTCCCTCCAATGATGGGAAGAGCAAGGATAAGCCTCCAAAGCGGAAGAAGGCAGACACTGAGG GGAAGTCCCCATCTCACAGTTCTTCTAATAGACCTTTCACCCCACCTACCAGCACGGGTGGGTCCAAATC CCCAGGCAGTTCAGGACGATCTCAGACGCCCCCAGGTGTTGCCACCCCGCCCATTCCCAAGATTACCATT CAGATTCCTAAAGGGACAGTGATGGTGGGCAAGCCCTCCTCTCACAGTCAGTACACTAGCAGTGGTTCTG TGTCTTCCTCTGGCAGCAAAAGCCACCATAGTCATTCTTCCTCCTCCTCTTCCTTAGCTTCTGCTTCCAC CTCAGGCAAGGTGAAAAGCAGTAAATCTGAAGGCTCATCAAGTTCCAAGCTCAGTGGCAGTATGTATGCT AGCCAAGGGTCTTCTGGATCCAGCCAGTCCAAAAATTCATCTCAGACTGGGGGGAAGCCAGGCTCCTCTC CCATTACCAAACATGGACTGAGCAGTGGGTCCAGCAGTACCAAGATGAAACCTCAAGGCAAGCCATCCTC CCTTATGAACCCTTCTATAAGTAAGCCAAACATATCCCCTTCCCATTCAAGGCCTCCCGGAGGCTCAGAT AAGCTTGCCTCTCCAATGAAGCCTGTTCCTGGAACCCCCCCATCCTCTAAAGCCAAGTCCCCTATCAGTT CAGGTTCCAGTGGTTCTCATGTGTCAGGAACTAGTTCAAGCTCTGGTATGAAGTCATCTTCAGGGTCAGC ATCCTCAGGCTCAGTGTCTCAAAAAACCCCTCCAGCATCTAATTCTTGTACACCATCTTCCTCTTCGTTT TCCTCAAGTGGTTCTTCCATGTCATCCTCTCAGAATCAACATGGCAGTTCCAAAGGGAAATCTCCCAGTA GGAATAAGAAGCCTTCCTTGACAGCTGTCATAGATAAATTGAAGCATGGGGTGGTTACCAGTGGGCCTGG GGGTGAGGATCCAATAGACAGTCAGATGGGCGCAAGCACAAATTCTTCTAACCATCCCATGTCCTCCAAA CATAACACGTCAGGAGGGGAGTTCCAGAGCAAACGTGAGAAAAGTGATAAAGACAAATCCAAGGTCTCTG CTTCTGGGGGGTCAGTGGATTCCTCTAAGAAGACTTCAGAGTCAAAAAATGTGGGGAGCACGGGGGTGGC AAAAATCATTATCAGCAAGCACGACGGAGGCTCCCCGAGCATCAAAGCCAAAGTGACGCTACAGAAACCT GGAGAAAGTGGTGGAGATGGGCTCAGGCCACAGATAGCCTCATCAAAGAACTATGGCTCTCCACTTATCA GTGGTTCCACTCCAAAGCACGAACGGGGTTCTCCCAGCCACAGTAAGTCGCCAGCATATACACCACAGAA TGTGGACAGTGAAAGTGAGTCAGGCTCCTCCATAGCAGAGAGATCCTACCAGAACAGTCCCAGCTCAGAG GATGGTATCCGACCACTTCCAGAGTACAGCACTGAGAAGCATAAGAAGCACAAAAAGGAAAAGAAGAAAG TCAGAGACAAAGACAGAGACAAGAAGAAGTCTCACAGCATGAAGCCAGAGAACTGGTCGAAATCCCCCAT TTCTTCAGATCCGACGGCGTCTGTGACAAATAACCCTATCTTATCTGCAGACAGGCCTTCTAGGCTCAGC CCTGACTTCATGATTGGGGAGGAAGATGATGATCTCATGGATGTGGCCCTGATTGGCAATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_013634 |
Insert Size | 4683 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013634.2, NP_038662.2 |
RefSeq Size | 6383 bp |
RefSeq ORF | 4683 bp |
Locus ID | 19014 |
UniProt ID | Q925J9 |
Cytogenetics | 11 61.75 cM |
Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Essential for embryogenesis, including development of the central nervous system, heart, liver and placenta and for erythropoiesis. Also required for normal transcriptional control of thyroid-stimulating hormone beta (TSHB) in the pituitary. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222482 | Med1 (tGFP-tagged) - Mouse mediator complex subunit 1 (Med1) transcript variant 1, (10ug) |
USD 1,668.00 |
|
MR222482 | Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 1 |
USD 1,468.00 |
|
MR222482L3 | Lenti ORF clone of Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 1 |
USD 1,768.00 |
|
MR222482L4 | Lenti ORF clone of Med1 (mGFP-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 1 |
USD 1,768.00 |
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