Carmil1 (NM_026825) Mouse Untagged Clone

CAT#: MC224360

Lrrc16a (untagged) - Mouse leucine rich repeat containing 16A (Lrrc16a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Carmil1
• Synonyms: 1110037D04Rik; AI425970; CARMIL; CARML1; D130057M20; Lrrc16; Lrrc16a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224360 representing NM_026825
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGACGAGAGCTCCGATGTCCCCCGGGAGTTAATGGAAAGCATCAAGGATGTTATTGGCAGGAAGA
  TCAAAATCTCAGTGAAGAAGAAAGTGAAGTTGGAAGTGAAGGGCGACAGGGTGGAAAACAAAGTGCTGGT
  GCTCACATCATGCCGAGCCTTCCTCCTGTCAGCACGCATCCCCAGCAAGCTCGAGTTAACCTTCAGTTAT
  CTAGAGATTCATGGAGTCATCTGCCACAAGCCAGCCCAGATGGTTGTGGAGACTGAGAAGTGTAACATGT
  CCATGAAGATGGTGTCCCCAGAGGATGTGAGTGAAGTGCTGGCTCACATAGGCACCTGCTTGCGGAGGAT
  ATTCCCAGGCCTTTCCCCACTGAGAATCATGAAGAAAGTCTCCATGGAGCCATCTGAACGTCTGGCCAGT
  CTCCAGGCCCTGTGGGACAGCCAGACCTTGGCTGAGCCAGGCCCCTGTGGTGGGTTTTCTCAGATGTATG
  CCTGTGTGTGCGACTGGCTGGGCTTTTCATACAAGGAAGAAGTGCAGTGGGATGTGGATACAATTTATCT
  GACACAAGACACCAGGGAATTAAATTTACAGGACTTCAGTCATCTCGAGCACAGAGACCTGATCCCCATC
  ATTGCTGCCCTGGAGTATAACCAGTGGTTCACCAAACTGTCCTCTAAGGATCTCAAACTGTCCACGGATG
  TTTGTGAACAGATTTTGAGAGTGGTGAGTAGGTCCAATCGACTGGAAGAGTTGGTGTTGGAAAATGCTGG
  ACTCAGAATAGATTTTGCACAGAAATTGGCCGGTGCTCTAGCACACAATCCTAACTCAGGACTTCACACA
  ATCAACCTTGCTGGGAACTCGCTGGAGGATCGAGGTGTGTCCTCCTTAAGTATTCAATTTGCCAAACTCC
  CAAAGGGATTGAAGCATTTGAACCTCTCTAAAACCTCATTGTCACCTAAAGGGGTGAACAGCCTTTGTCA
  GTCCCTCAGTGCCAACCCTCTGACTGCCTCTACCCTCACCCACCTGGACCTCTCAGGGAATGCCCTCCGC
  GGAGATGACCTCTCGCACATGTACAATTTTTTGGCCCAGCCCAACACCATTGTTCATCTGGATCTATCTA
  ATACAGAATGTTCTCTGGAGATGGTGTGTAGCGCTCTCCTCCGGGGTTGCCTTCAGTGTCTCGCTGTGCT
  CAATCTCTCCAGATCTGTCTTCTCTCACAGGAAAGGAAAGGAAGTCCCTCCGTCTTTCAAGCAGTTTTTC
  AGCAGTTCTCTGGCTTTGATCCAAATCAACCTTTCAGGCACGAAGCTGTCTCCCGAGCCCTTGAAAGCGC
  TGCTCTTGGGCCTGGCCTGCAATCATAGCCTGAAGGGAGTCTCTCTGGATCTCAGCAACTGTGAGCTTGG
  CCACTGTCTGAGGTCAGGAGGAGCTCAAGTGTTAGAAGGCTGCATCGCTGAAATCCACAACATCACCAGC
  TTAGATATCTCTGACAATGGTTTAGAATCCGACCTATCTACCCTAATTGTGTGGCTCAGTAAAAACAGAT
  CAATACAGCACCTGGCATTGGGCAAAAATTTTAATAATATGAAATCCAAAAATCTGACGCCTGTGTTGGA
  CAACTTAGTGCAGATGATCCAAGATGAAGACTCTCCTCTGCAGTCGTTGTCCCTGGCTGACTCAAAACTC
  AAGGCTGAGGTCACCATCATCATCAATGCCCTGGGCAGCAACACCTCCCTGACCAAAGTGGACATCAGTG
  GGAATGGCATGGGGGACATGGGAGCGAAGATGCTGGCCAAGGCTCTCCAGATCAACACCAAACTCAGGAC
  AGTCATCTGGGACAAAAACAACATCACTGCACAAGGCTTTCAAGACATAGCTGTTGCTATGGAAAAGAAC
  TACACACTGCGATTTATGCCAATTCCGATGTATGATGCCGCTCAAGCTCTCAAAACCAACCCCGAGAAAA
  CAGAAGAGGCTCTGCAGAAGATAGAAAATTATCTGCTCCGGAATCACGAGACCAGGAAATACCTTCAGGA
  GCAGGCCTATCGCCTCCAGCAGGGCATCGTCACCAGCACCACCCAGCAGATGATTGACCGGATATGTGTG
  AAGGTTCAGGATCACCTCAACTCCTTACGAGCCTGTGGGGGTGATGCTATTCAGGAGGATTTAAAGGCAG
  CGGAGCGGCTCATGCGAGATGCTAAGAACTCTAAAACATTGTTGCCCAATTTATATCATGTGGGCGGTGC
  ATCCTGGGCAGGAGCCAGTGGGCTGTCATCCAGTCCCATTCAGGAGACCCTGGAGTCGATGGCTGGAGAA
  GTCACAAGAGTGGTGGATGAACAACTGAAGGATTTGCTAGAATCCATGGTGGACGCAGCCGAGACCCTCT
  GCCCCAACGTCATGAGAAAAGCCCACATCCGACAGGACCTGATCCATGCCAGCACCGAAAAGATTTCCAT
  CCCACGTACCTTTGTTAAAAATGTCCTGTTGGAACAGTCCGGGATTGACATTCTTAACAAAATCAGTGAG
  GTGAAGCTGACAGTGGCCTCGTTCTTGTCTGACCGGATCGTCGATGAGATCCTGGATTCTCTCTCCAGCA
  GCCATCGGAAGCTGGCCAACCATTTCAGCAGGCTAAACAAGAGCCTTCCCCAGCGAGAGGACCTAGAGGT
  GGAGCTGGTGGAGGAGAAGCCGGTGAAACGGGCCATCCTCACGGTAGAGGATCTCACAGAGGTGGAGAGG
  CTGGAAGACCTAGACACTTGTATGATGACTCCTAAGTCCAAGAGGAAGAGCATCCACAGCCGAATGCTGC
  GGCCTGTCTCCAGGGCCTTCGAAATGGAGTTTGATCTTGATAAAGCCCTGGAAGAGGTGCCGATTCACAT
  CGAAGACCCACCTTTCCCATCAGTCCGGCAAGAGAAGCGAAGCTCGGGGTTAATCTCTGAGCTGCCCTCG
  GAGGAAGGCAGGAGACTGGAACACTTCACCAAGTTAAGGCCCAAGCGGAACAAGAAGCAGCAACCTACGC
  AAGCAGCGGTCTGTACCATCAGCATCTTGCCCCAGGATGGAGAACAGAATGGCCTCATGGGCAGAGTGGA
  TGAAGGGGTAGATGAGTTTTTCACCAAGAAGGTGACTAAAATGGATTGCAAGAGGTCATCCTCGCGCTCC
  TCAGATGCCCACGAACTCGGGGAAGGAGATGAGAAGAAAAAACGGGACTCTCGGAGGAGTGGCTTCCTCA
  ACCTGATTAAATCCAGGTCCAGATCTGAGCGGCCACCCACAGTCCTGATGACGGAAGAGCTCTCCTCCCC
  GAAAGGGGCAATGCGGAGCCCACCAGTGGATACCACCAGGAAGGAGATAAAGGCAGCGGAGCACAATGGT
  GCTCCAGACCGCACAGAAGAGATTAAGACACCGGAACCCCTGGAGGAGGGTCCAGCAGAGGAAGCTGGCA
  GGGCTGAGCGCAGTGACAGCAGGGGCAGCCCGCAGGGTGGCCGGCGCTATGTGCAGGTGATGGGCAGCGG
  CCTGCTGGCCGAGATGAAGGCCAAGCAGGAGCGGAGGGCAGCATGTGCACAGAAGAAGCTCGGCAATGAT
  GTCATCTCCCAGGATCCTTCCAGCCCAGTCTCGTGCAACACAGAGCGTTTAGAAGGAGGGGCGACAGTGC
  CTAAACTGCAACCAGGTCTTCCAGAGGCCCGCTTTGGTTCGGGAACACCAGAAAAGAATGCCAAAGCTGA
  ACCCAGAGTGGATGGAGGCTGCAGGTCCCGGAGCTCCTCCAGCATGCCCACCAGCCCAAAGCCCCTCCTT
  CAGTCCCCTAAGCCCAGCCCTTCAGCCCGGCCTTCTATCCCTCAGAAGCCAAGAACTGCCTCCCGACCTG
  AAGACACCCCAGACTCTCCATCTGGTCCTAGTTCCCCTAAAGTTGCCCTCCTTCCACCCATCCTCAAAAA
  AGTCTCCTCGGACAAGGAGCGTGACGGCCAGAACAGCTCACAGTCCAGTCCTAGGAGCTTCTCCCAGGAA
  GCCTCAAGGAGAAGCTGGGGCCCGGCCCAGGAGTATCAAGAACAGAAGCAACGGTCCTCGGGCAAAGATG
  GTCACCAAGGGAGCAAATGCAGTGACTCTGGAGAAGAGGCAGAAAAAGAGTTTATTTTTGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026825
• Insert Size: 4125 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026825.3, NP_081101.3
• RefSeq Size: 5158 bp
• RefSeq ORF: 4125 bp
• Locus ID: 68732
• UniProt ID: Q6EDY6
• Cytogenetics: 13 A3.1
• Gene Summary: Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization, however, seems unable to nucleate filaments (PubMed:16054028). Plays a role in lamellipodial protrusion formations and cell migration (PubMed:16054028).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).