Cemip (NM_030728) Mouse Untagged Clone

CAT#: MC224329

9930013L23Rik (untagged) - Mouse RIKEN cDNA 9930013L23 gene (9930013L23Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cemip
• Synonyms: 12H19.01.T7; 6330404C01Rik; 9930013L23Rik; AY007814; Kiaa1199
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224329 representing NM_030728
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGCTTCTGGGAGGCACGATGTCTCCCTCAAGATTGTGCTGGCCACGGGCTGCCTCCTCCTGGCCA
  ACTTCTCAGGGGCCTCATCTGCAGTGGCAACTGAGTGTCCTGATCAGAGTCCTGAGCTGCAGCCTTGGAG
  CCCTGGCCATAACAGAGACTATCAAGTACACATTGGCCATGGCAGGAAACTACTGCTCACCTCTTCTGCC
  ACAGTCCACTCCATCACTATCTCAGGGGGAGGAAAGCTTGTCATTAAAGACCACCATGAGCACATTGTGC
  TGCGTACCCGGTACATCCTGATTGATGACGGTGGAGAGCTGCATGCTGGGAGTGCCCTTTGCCCTTTTGA
  GGGCAATTTCAGTATTGTGCTGTATGGAAGGGCTGATGAAAACATCCTGCCAGACCCTTACTATGGCCTG
  AAGTACATCGGAGTAGACAAAGGAGGCACTCTTGAATTACATGGGCAGAAAAAGCTTTCCTGGACTTTTC
  TAAACAAGACCCTTCATCCTGGTGGCATGCAGGAGGGAGGATATTTTTTTGAAAGGAGCTGGGGCCACCG
  TGGAGTCATTGTCCATGTCATTGATGCCAAATTGGGCACAGTTGTCCATTCTGACCGGTTTGACACTTAC
  AGATCCAAAAAAGAGAGTGAACGTCTGGTCCAGTATTTGAATGCAGTGCCCGATGGCAGGATTCTTTCTG
  TCGCAGTGAATGATGAAGGCTCTAGAAACCTGGATGACACAGCCCGGAAGGCAATGACCAAACTGGGCAG
  CAAGCACTTCCTCCACCTTGGATTTAGACACCCTTGGAGCTTCATCACTGTGAAAGGAAATCCCTCCTCT
  TCAGTTGAAGACCACATTGAATACCATGGTCACAAAGGCTCGGCTGCTGCCCGGGTTTTCAAACTGTTCC
  AGACAGAGCATGGTGAACATTTCAATGTTTCTTCATCCAGCGAATGGGTTCAAGATGTGGAATGGACAGA
  GTGGTTTGACCATGATAAGGTGCCTCAGAGCAAAGGTGGGGAGAAAATTTCAGATCTGCGAGCAGCTTAC
  CCAGGAAAAATCTGCAATCGACCCATTGATATACAGGCCACAACAATGGATGGAGTCGCCCTCAGCACTG
  AGGTTGTCTACAAAAATGGCCAAGATTACAGGTTTGCTTGCTACACCCGGGGCAGAGCCTGCCGAAGCTA
  TCGAGTGCGATTCTTATGTGGAAAACCCGTGAGGCCCAAGCTCACGGTTAGCATCGACACCAACGTAAAC
  AGCACCATCCTGAGTCTGGTGGACAATGTGCGGTCATGGAGGCCTGGAGACACCCTGGTTGTTGCCAGCA
  CTGACTATTCGATGTACCAGGCAGAAGAGTTCCGGGTGCTCCCCTGCAAAGCTTGCACCTCTACACAGGT
  CAAGGTGGCAGGGAAACCACAGTACCTACATATTGGGGAGGAGATTGATGGTGTAGACATGCGGGCTGAG
  GTCGGACTCCTGACTCGGAACATTGTGGTGATGGGGGAGATGGAGGACAGATGCTACCCCTACACCAATC
  ACATCTGTGACTTCTTTGACTTCGATACCTTTGGGGGCCACATCAAGTTTGCACTGGGGTTTAAAGCAGC
  CCACTTGGAGGGCGTGGAGCTGAAGTACATGGGGCAGCAGCTGGTGGGCCAGTACCCAATTCACTTCCAC
  CTGGCTGGAGATTTGGATGAACAGGGAGGCTATGACCCCCCTACCTACATCAGGGACCTCTCCATTCATC
  ACACATTCTCCCGCTGCATCACTGTCCATGGCTCCAACGGTCTGTTGATCAAGGATGTTGTGGGCTATAA
  TTCTTTGGGCCACTGCTTCTTCACTGAAGATGGGCCAGAGGAACGCAACACTTTTGACCACTGCCTTGGC
  CTCCTTGTTAAGTCAGGGACCCTTCTCCCATCCGATCGGGATAGCAGGATGTGCAAAGTGATCACAGAGG
  ACTCATACCCAGGGTACATCCCCAAGCCCAGGCAGGACTGCAATGCCGTGTCCACATTCTGGATGGCGAA
  TCCCAACAACAATCTCATCAACTGCGCAGCTGCAGGGTCCGAGGAAACTGGATTTTGGTTCATTTTTCAC
  CATGTGCCAACGGGCCCCTCTGTGGGAATGTACTCTCCAGGTTATTCTGAGCACATTCCACTGGGTAAAT
  TCTACAACAATCGAGCACATTCTAACTACCGGGCTGGCATGATCATAGACAACGGAGTCAAGACAACTGA
  GGCCTCAGCCAAGGATAAACGGCCCTTCCTCTCCATCATCTCTGCCAGGTACAGCCCTCACCAGGACGCA
  GACCCACTGAAACCTCGGGAACCAGCCATTATCCGACACTTTACTGCCTACAAGAACCAGGATCATGGAG
  CCTGGCTGCGGGGTGGGGATGTGTGGCTGGACAGCTGTCGGTTTGCTGATAATGGCATTGGTCTGACCCT
  GGCCAGTGGTGGAACCTTCCCATATGATGATGGCTCCAAGCAGGAGATAAAGAACAGCTTGTTTGTTGGC
  GAGAGTGGCAATGTGGGCACAGAAATGATGGACAACAGGATCTGGGGTCCAGGTGGCTTGGACCACAGTG
  GAAGGACCCTCCCTATTGGCCAGAATTTTCCCATTAGAGGAATTCAGTTCTACGATGGCCCCATCAACAT
  CCAGAACTGCACCTTCCGCAAGTTCGCGGCCCTCGAGGGCAGGCACACCAGTGCCTTGGCCTTCCGCCTA
  AACAATGCCTGGCAAAGCTGCCCTCACAACAATGTGACCAACATAGCCTTTGAGGATGTCCCGATTACTT
  CCAGAGTGTTCTTCGGAGAGCCCGGGCCCTGGTTCAACCAGCTGGACATGGACGGGGACAAGACATCTGT
  GTTCCATGATCTGGACGGCTCTGTGTCGGAGTACCCTGGCTCCTATCTCACTAAGGACGACAACTGGCTG
  GTCCGACACCCAGACTGCATCAATGTCCCAGACTGGAGAGGGGCCATCTGCAGTGGGCGCTATGCACAGA
  TGTACATCCAGGCCTACAAGAGCAGCAATCTGCGCATGAAGATCATTAAGAATGACTTCCCCAGCCACCC
  GCTCTACCTGGAGGGGGCCCTCACCAGGAGCACCCACTACCAGCAGTATCAGCCTGTCATCACCCTGCAG
  AAGGGCTATACTATCCACTGGGACCAGACAGCCCCCGCCGAGCTTGCCATCTGGCTCATCAACTTCAACA
  AGGGTGACTGGATCCGAGTGGGGCTCTGCTACCCAAGAGGCACCACCTTTTCCATCCTTTCAGATGTTCA
  CAATCGCCTGCTAAAGCAAACGTCCAAGACTGGGACCTTTGTGAGGACTCTGCAGATGGACAAAGTTGAG
  CAGAGCTATCCTGGCAGGAGCCACTACTACTGGGATGAGGATTCTGGGTTGCTCTTCTTGAAATTAAAAG
  CCCAGAACGAAAGGGAGAAATTTGCTTTCTGCTCCATGAAGGGCTGTGAGAGAATCAAGATTAAAGCTCT
  GCTCCCAAGGAATGCAGGCATCAGCGACTGCACAGCCACGGCTTACCCCAGGTTCACTGAGAGGGCCATA
  GTGGATGTGCCAATGCCCAGGAAGCTCTTTGGGGCTCAGCTGAAGACAAAAGACCACTTCCTGGAAGTGA
  AGATGGAAAGTTCCAGGCAGCACTTCTTCCACCTTCGGAATGACTTCGCTTATATTGAAGTGGATGGGAG
  GAGGTACCCTTGCTCAGAAGATGGCATCCAGATAGTGGTGATTGATGGGAGTCGAGGTCACGTGGTGAGC
  CACGGCAGCTTCAGAAATGCCATTCTGCAGGGCATACCCTGGCAGCTCTTCAACTACGTGGCAGCTATCC
  CTGACAATTCCATAGTGCTCATGGCATCAAAGGGCAGATACATCACCAGAGGCCCATGGACCAGGGTGCT
  GGAAAAGCTTGGGGCAGACAAAGGTCTCAAGTTGAAAGAAAAGATGGCGTTCGTCGGCTTCAAAGGCAGC
  TTCCGACCCATCTGGGTGACTCTGGAGACTGAGGACCACAAAGCCAAAATCTTCCAAGTGGTGCCCATCC
  CTGTGGTGAGAAAGAAGAAGCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030728
• Insert Size: 4086 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030728.4, NP_109653.3
• RefSeq Size: 7114 bp
• RefSeq ORF: 4086 bp
• Locus ID: 80982
• UniProt ID: Q8BI06
• Cytogenetics: 7 48.35 cM
• Gene Summary: Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.[UniProtKB/Swiss-Prot Function]