Shank2 (NM_001081370) Mouse Untagged Clone

CAT#: MC224114

Shank2 (untagged) - Mouse SH3/ankyrin domain gene 2 (Shank2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Shank2
• Synonyms: mKIAA1022; ProSAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224114 representing NM_001081370
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGCGTCCCCGGCGGTGGAGCAGCCACCGTGATGATGACCGGTTACAATAATGGTCGCTATCCCC
  GGAATTCTCTCTACAGTGACTGCATTATTGAGGACAAGACGGTGGTCCTGCAAAAGAAGGACAACGAGGG
  CTTTGGATTTGTGCTCCGAGGGGCAAAAGCCGATACCCCCATTGAGGAATTCACACCCACGCCAGCATTT
  CCAGCCCTGCAGTACCTGGAGTCCGTGGATGAAGGTGGGGTGGCATGGCAAGCCGGACTAAGGACCGGGG
  ACTTCTTGATTGAGGTTAACAATGAAAATGTCGTCAAGGTGGGCCACAGGCAGGTGGTGAACATGATCCG
  CCAGGGAGGGAATCACCTCATCCTTAAGGTCGTCACGGTGACCAGGAATCTAGACCCTGATGATACAGCC
  AGAAAGAAAGCTCCCCCACCTCCAAAGCGGGCTCCGACCACGGCCCTCACCCTGCGTTCCAAGTCCATGA
  CAGCGGAGTTGGAGGAACTCGTGGACAAAGCCTCAGTCCGGAAGAAGAAGGATAAACCGGAAGAGATAGT
  CCCAGCCTCCAAGCCCTCCAGGACTGCAGAGAACGTGGCCATCGAATCCAGGGTGGCGACCATCAAGCAG
  CGGCCCACAAGCCGGTGCTTCCCAGCTGCCTCTGATGTGAATTCCGTGTACGAGCGCCAAGGGATTGCTG
  TAATGACGCCCACGGTCCCTGGGAGCCCGAAAGGCCCATTTCTGGGCCTCCCTCGAGGTACGATGCGAAG
  GCAGAAATCGATAGACAGCAGAATCTTTCTATCAGGGATAACAGAGGAAGAGCGGCAGTTTCTGGCTCCC
  CCAATGCTGAAGTTCACCCGAAGCTTGTCCATGCCAGACACTTCTGAGGACATCCCCCCTCCGCCACAGT
  CTGTGCCCCCCTCTCCCCCTCCTCCTTCCCCCACCACATACAACTGTCCCAGGTCCCCGACTCCAAGAGT
  CTATGGGACAATTAAGCCCGCGTTCAATCAGAACCCCGTCGTCGCCAAGGTGCCCCCAGCCACCAGGTCT
  GACACTGTGGCCACCATGATGCGGGAAAAGGGGATGTTCTACAGGAGAGAGCTGGACAGATTTTCCCTGG
  ACTCAGAAGACGTCTACAGCCGCAGCCCCGCCCCACAGGCCGCCTTCCGCACCAAGCGGGGACAGATGCC
  TGAGAACCCGTACTCAGAGGTGGGAAAGATAGCCAGCAAGGCCGTCTATGTCCCTGCCAAGCCAGCCAGG
  CGGAAGGGCGTGCTGGTAAAGCAGTCCAACGTGGAGGATAGCCCCGAGAAGACGTGCTCCATACCCATCC
  CAACCATCATCGTCAAGGAACCCTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGGAGCAGCATGGAGAT
  TGACCCCCAGGCCACCGAGCCCGGCCAGCTGCGGCCAGATGACAGCCTCACCGTCAGCAGCCCCTTTGCT
  GCGGCCATCGCTGGGGCTGTGCGTGACCGGGAGAAGCGTCTGGAAGCCAGGAGGAATTCCCCAGCCTTCC
  TCTCCACCGACCTGGGAGATGAGGACGTGGGTCTGGGGCCGCCTGCTCCCCGGATGCAGGCCTCCAAGTT
  CCCAGAGGAGGGTGGGTTTGGTGACGAGGATGAGACGGAACAACCGCTGTTGCCTACCCCCGGGGCAGCA
  CCCAGGGAGCTGGAGAATCACTTCCTAGGTGGTGGTGAGGCTGGTGCTCAGGGGGAGGCTGGGGGACCCC
  TGAGTTCCACATCCAAAGCCAAGGGGCCTGAGAGTGGCCCAGCAGCCCCCCTCAAGAGCAGCAGCCCAGC
  CGGCCCTGAGAATTACGTGCACCCACTCACAGGGCGGCTGCTTGACCCCAGCTCCCCACTGGCCCTGGCA
  CTCTCAGCCAGGGACCGAGCCATGCAGGAGTCCCAGCAGGGGCACAAAGGAGAGGCCCCCAAGGCTGACC
  TTAACAAGCCTCTCTACATCGATACCAAAATGCGGCCCAGCGTGGAGTCCGGCTTTCCTCCAGTCACCAG
  ACAGAACACCAGGGGTCCCCTGCGACGGCAAGAGACAGAGAACAAGTACGAGACAGACCTGGGCAAGGAC
  CGGAGGGCGGACGACAAGAAGAACATGCTGATCAACATCGTGGACACTGCCCAGCAGAAGTCAGCTGGCC
  TACTGATGGTGCACACAGTGGACGTCCCCATGGCCGGGCCACCCCTGGAGGAAGAGGAGGACAGAGAGGA
  TGGGGATACAAAGCCAGACCACTCACCCTCCACAGTGCCAGAAGGCGTTCCCAAAACCGAAGGTGCTTTA
  CAGATCTCCGCTGCCCCGGAGCCCGCCGTCGCGCCCGGCAGGACCATCGTGGCGGCGGGCTCCGTGGAAG
  AGGCGGTGATTCTGCCATTCCGCATCCCCCCTCCCCCTCTGGCATCCGTGGACTTGGATGAGGACTTTCT
  TTTCACAGAACCGTTGCCTCCCCCCCTGGAATTCGCCAATAGTTTTGATATCCCCGATGACCGGGCAGCT
  TCAGTTCCCGCTCTGGCTGACCTGGTCAAGCAGAAGAAAAACGACACCCCTCAGCCCCCTACGTTGAACT
  CCAGCCAACCAGCCAACTCCACAGACAGTAAGAAGCCAGCCGGTATCTCGAACTGTCTGCCCTCCTCGTT
  CCTGCCACCCCCCGAAAGTTTCGATGCAGTCACCGACTCGGGGATTGAGGAGGTGGACAGCCGGAGTAGC
  AGCGACCACCACCTGGAGACTACCAGCACCATCTCCACGGTGTCCAGCATCTCCACGCTGTCCTCAGAGG
  GCGGCGAGAGCATGGACACCTGCACAGTCTATGCAGACGGGCAAGCCTTTGTGGTTGACAAGCCCCCAGT
  ACCTCCAAAGCCAAAAATGAAGCCCATCGTTCACAAGAGCAACGCACTTTACCAAGACACGCTCCCAGAA
  GAGGACACGGATGGCTTTGTGATCCCCCCACCTGCACCCCCGCCCCCGCCGGGCAGCGCCCAGGCCGGTG
  TGGCGAAGGTCATCCAGCCAAGGACCTCCAAGTTGTGGGGTGACGTTCCAGAGGTCAAAAGCCCAATTCT
  CTCAGGCCCAAAGGCAAATGTCATTAGTGAGCTAAACTCCATTCTGCAGCAGATGAACAGGGGGAAATCG
  GTCAAGCCCGGGGAAGGGCTGGAGCTGCCGGTGGGAGCCAAGTCGGCCAACCTCGCTCCAAGAAGCCCGG
  AGGTCATGAGCACCGTCTCAGGTACACGGAGCACGACGGTCACCTTCACTGTCCGCCCTGGCACCTCCCA
  GCCCATCACCCTGCAGAGCCGGCCCCCCGACTATGAAAGCAGAACCTCAGGACCTAGACGCGCCCCAAGC
  CCTGTGGTTTCACCAACGGAATTGAGCAAAGAGATCCTGCCCACCCCTCCCCCTCCGTCCGCCACTGCAG
  CCTCTCCCTCCCCCACACTCTCAGATGTCTTTAGCCTTCCGAGCCAGTCCCCTGCAGGGGACCTCTTTGG
  CTTGAACCCAGCAGGACGGAGCAGGTCACCATCTCCTTCAATATTGCAACAGCCAATCTCAAATAAGCCT
  TTTACAACTAAGCCTGTCCACCTGTGGACGAAACCAGATGTGGCAGACTGGCTGGAAAGTCTGAACTTGG
  GTGAACACAAGGAGACGTTCATGGACAATGAGATTGACGGCAGCCACCTGCCAAACCTTCAGAAGGAAGA
  CTTGATAGATCTTGGGGTGACTCGAGTTGGGCATAGGATGAACATAGAAAGGGCTTTGAAACAGCTGCTG
  GACAGATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081370
• Insert Size: 3789 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081370.2, NP_001074839.2
• RefSeq Size: 5913 bp
• RefSeq ORF: 3789 bp
• Locus ID: 210274
• UniProt ID: Q80Z38
• Cytogenetics: 7 F5
• Gene Summary: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses an alternate downstream promoter compared to variant 2 and encodes a shorter isoform (a) with a distinct N-terminus compared to isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.