Palld (NM_001081390) Mouse Untagged Clone

CAT#: MC223618

Palld (untagged) - Mouse palladin, cytoskeletal associated protein (Palld), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Palld
• Synonyms: 2410003B16Rik; 6030492A02
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223618 representing NM_001081390
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGACTTCCTCCCACGACTCCTTCTATGACTCCTTATCAGACGTGCAGGAAGAGGGCAAAAGTG
  CTGACTTCTTTCCAGGCCTCTCTGCTTTCCTCAGCCAGGAAGAAATAAATAAAAGCCTTGACCTGGCACG
  CAGAGCTATAGACAGCTCTGAAACTGAAGATTTTGACTCTGAGAAGGAGATCTCGCAGATTTTCAGCAAG
  TCTCCCATAAGCCTCTGCGAAACTCCTTCCCATGAGGAGCCGAAATCAGGCAAGCAGACTTCCTCAGAAC
  GACCTCAGGATAGCAGGCGAGCACCTGTCCAGCCTCTAACAGGAGATCAAGCTGAAAGGATCACTTCCCC
  AGGTTCAAAGAGAAAACCCGGGGTATCACCCCTGCTGGCCAGCCCCAGCTACATCCGGAGCCTCCGCAAG
  GCTGAAAAACGAGGTGCAAAAAATCCCAATCCAAGTTCAAAGCCCAAAACTGCCCAGCAAAGCAAGGCTG
  GCCCCCAGAGCCAACTGTGCGACAAGGCGGCTAGTTTCATCGAGGAACTGACCTCCATATTTAGAGAAGC
  GGCAAAGCCAAGGAACAGAAGCCCAAATGGTGAGTCCTCATCACCAGACAGTGGGTATCTGTCTCCTAAA
  AATCAGCCATCAGCCCTGATGAGTGCCTCAGCCAGTCAGAGCCCCACTGCAGACCAGCTAGATCAACTGG
  AGATGGACGCAGAAGTCAAGCAAGCCCAGGGCAGCCTTTGCTACCAGGCCCACCAGGCCTCGGAAGAGAC
  CTTGCCACTCGCTCACATCCCGCACCCACAGCCCCAGAAAGCCCGCCACTTGCCAACTGCACCTAGGTTC
  ATCCAGAAACTGAGGAGCCAAGAAGTTGCTGAAGGAAGCAGAGTTTATTTGGAGTGTAGAGTCACAGGAA
  ACCCAACGCCCAGGGTCAGATGGTTCTGTGAGGGGAAGGAGCTGTACAACAGTCCTGACGTTCAGATCCA
  CTGCGAGAGTGGGGAGCTGCACACCTTGGTCATTGCTGAGGCCTTTGAGGACGACACAGGTCGCTACACG
  TGTCTGGCTACAAACCCCAGTGGCTCAGACAGCACATCTGCAGAGGTGTTCATTGAAGGAGCCAGTTCAA
  CGGACTCTGACAGCGAAAGCTTATCTTTCATATCAAAAGCTGGAGCCATGCCACAAGCTCAGAAGAAAAC
  AACGTCCGTTTCCTTAACGATAGGATCCTCAGCTCCGAAAACAGGAGTGACCACAGCTGTGATTCAGCCC
  CTATCTGTGCCCGTCCAACAGGCTCACAGCGCTACTTCATATCTCTGCCGACCTGACGGAACCACCATGG
  GCTGCCTTCTTCCTGTTTTTACTAAGGAACTACAGAACACAGCAGCCTCCGAGGGCCAGGTGGTGGTGCT
  GGAATGCAGAGTCCGAGGGGCACCCCCGCTGCAGGTCCAGTGGTTCCGGCAAGGAAGTGAGATCCAAGAC
  TCTCCGGACTTCAGAATTCTCCAGAAAAAACCCAGATCGACAGCTGAACCCGAGGAGATCTGCACCCTTG
  TCATTGCTGAGAGTTTCCCTGAAGATGCAGGCATCTTTACCTGCTCAGCCACAAATGACTACGGCTCAGT
  GACCAGCACTGCACAGCTTGTTATAACCTCCGCCAACAACGAGAACTGTAGTTACGACTCAACAGGAGAA
  CCCAACAGTGATCACTTCCAACACTTTCCACCTCCCCCTCCGATCCTGGAGACAGGCTCCTATGAGCTGG
  CATCCCAGAAACCATCTGAAATCCAGCAGGTGAACAGCCCCAATTTAGGATTTAGCATGGCAGCTCTTCA
  AATGCAATTCAACACTGCAGAGAGGGAGACCAACGGAGTCCATCCCAGCCATGGAGTCAACGGGCTGATT
  AATGGCAAAGCTTATGGCAATAAATCTCCTCCAACACCAACTGCCCTGCTTTCACCCACTAAGGAACCAC
  CGCCACTCCTTGCCAAACCCAAACTGGGGTTCCCAAAGAAGTCCAGTAGAACTGCTAGAATTGCCTCTGA
  TGAGGAGATTCAAGGCACAAAGGATGCTGTCATCCAAGACCTGGAACGGAAGCTTCGCTTCAAGGAGGAC
  CTTCTGAACAATGGCCAACCGAGGCTAACCTATGAGGAAAGAATGGCTCGCCGCCTGCTTGGAGCCGACA
  GCGCAAACGTCTTCAACATCCAGGAGCCAGAGGAAACGGCAGCCAATCAGGAGTACAAAGTCTCTAGCTG
  CGAGCAGAGGCTGATTAGCGAGATTGAGTACCGGCTGGAGCGCTCCCCTGTGGATGAGTCGGGAGACGAG
  GTGCAGGATCCAGATGTGCCTGTGGAGAACGCAACAGCTCCCTTCTTTGAGATGAAGCTGAAACACTACA
  AGATCTTTGAGGGGATGCCGGTGACTTTCACGTGTCGAGTGGCTGGGAATCCAAAGCCAAAGATCTATTG
  GTTTAAAGATGGGAAGCAGATTTCTCCGAAGAGCGATCACTACACCATTCAGAGAGACCTTGATGGGACC
  TGCTCTCTCCACACCACGGCCTCTACCCTAGACGACGATGGGAACTACACCATCATGGCTGCCAACCCTC
  AGGGTCGCGTCAGTTGTACAGGAAGGCTAATGGTACAGGCTGTCAACCAAAGAGGCCGCAGTCCCCGCTC
  TCCCTCAGGCCATCCTCATGCCAGAAGGCCTCGCTCTCGATCACGGGACAGTGGAGATGAAAACGAGCCC
  ATTCAGGAGCGATTCTTCAGACCTCACTTCCTGCAGGCTCCTGGAGACCTGACCGTTCAGGAAGGCAAGC
  TCTGCAGGATGGACTGCAAGGTCAGTGGGTTACCAACCCCAGATCTCAGCTGGCAACTAGATGGAAAGCC
  CATACGCCCCGACAGTGCTCACAAGATGCTGGTCCGTGAGAATGGGGTCCACTCCCTCATTATAGAGCCA
  GTCACGTCCCGGGACGCCGGCATCTACACATGTATTGCCACCAACAGAGCAGGACAGAACTCGTTTAACC
  TGGAGCTTGTGGTTGCTGCTAAGGAAGCACACAAGGCCCCTGTGTTTATGGAGAAGCTACAGAACACGGG
  GGTTGCTGATGGATACCCAGTGCGGCTGGAATGCCGTGTCTCGGGAGTGCCGCCACCTCAGATATTTTGG
  AAGAAAGAAAATGAATCGCTCACTCACAGCACTGAGCGAGTAAGCATGCACCAGGATAATCATGGCTACA
  TCTGCCTGCTCATCCAGGGAGCCACAAAGGAAGACGCTGGGTGGTACACTGTGTCCGCCAAGAACGAAGC
  AGGCATTGTGTCCTGCACTGCCCGGCTGGATGTCTACATTTCCCGACATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081390
• Insert Size: 3342 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081390.1, NP_001074859.1
• RefSeq Size: 3566 bp
• RefSeq ORF: 3342 bp
• Locus ID: 72333
• UniProt ID: Q9ET54
• Cytogenetics: 8 B3.1
• Gene Summary: Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific May be required for the initiation of neural tube closure.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks two alternate in-frame exons and differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (4) lacks two alternate internal segments and has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.