Ank2 (NM_178655) Mouse Untagged Clone

CAT#: MC223346

Ank2 (untagged) - Mouse ankyrin 2, brain (Ank2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ank2
• Synonyms: 100043364; AI835472; Ank-2; AW491075; Gm4392
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223346 representing NM_178655
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGAGGTCCTTGATGTTTCTGATGAAGAGGGTGATGACACTGTGACAGGTGATGGGGGAGAATACC
  TCAGGCCAGAAGATCTCAAGGAGCTCGGAGATGACTCACTGCCCAGCAGTCAGTTCCTGGATGGCATGAA
  CTACCTTCGGTACAGTCTGGAGGGAGGAAGATCCGACAGCCTCCGGTCCTTCAGTTCCGACAGGTCTCAC
  ACTCTGAGCCATGCATCGTACCTGAGGGACAGTGCCATGATTGACGACACGGTTGTGATCCCCAGCCACC
  AGGTGTCTGCGCTAGCCAAGGAGGCAGAAAGGAATTCTTATCGTCTGAGCTGGGGCACTGAGAACTTAGA
  CAACGTGGCTCTTTCTTCCAGTCCTATTCATTCAGGTTTCCTAGTCAGTTTTATGGTGGATGCCCGTGGT
  GGTGCCATGCGAGGATGCAGACACAATGGACTCAGAATCATTATCCCACCTCGGAAATGCACAGCCCCAA
  CTCGCGTCACCTGCCGCCTTGTGAAACGCCATCGACTGGCAACAATGCCGCCCATGGTGGAAGGAGAAGG
  CCTGGCCAGCCGCCTGATTGAAGTCGGACCTTCGGGAGCTCAGTTTCTTGGGCCCGTGATCGTGGAGATC
  CCTCACTTCGCCGCTCTTCGAGGAAAGGAGAGGGAGCTGGTGGTCCTGCGCAGTGAAAATGGGGACAGCT
  GGAAAGAGCATTTCTGCGACTACACTGAGGATGAATTGAACGAAATCCTTAATGGCATGGATGAAGTGCT
  GGACAGTCCAGAAGACCTGGAAAAGAAACGAATCTGCCGCATCATCACTCGTGACTTCCCACAGTACTTT
  GCGGTGGTGTCTCGCATCAAACAGGACAGCAACCTGATTGGCCCCGAGGGTGGAGTACTGAGCAGCACAG
  TGGTGTCACAGGTGCAAGCCGTCTTCCCAGAGGGCGCACTCACCAAGCGCATTCGAGTAGGCCTACAGGC
  TCAACCTATGCATAGTGAATTGGTAAAGAAGATCTTAGGCAACAAAGCCACCTTCAGCCCAATAGTCACT
  TTGGAACCCAGGAGAAGAAAATTCCACAAGCCAATTACTATGACTATTCCTGTCCCCAAAGCTTCAAGTG
  ATGTCATGCTGAATGGTTTTGGGGGAGACGCACCAACCTTAAGATTACTGTGCAGCATAACAGGTGGAAC
  TACGCCTGCCCAGTGGGAAGACATCACAGGAACAACGCCACTAACATTTGTCAATGAGTGTGTTTCCTTT
  ACAACCAACGTGTCTGCCAGGTTCTGGCTGATAGACTGTCGACAGATTCAGGAGTCTGTTGCCTTTGCAT
  CACAAGTGTATAGAGAAATTATCTGTGTGCCATATATGGCCAAATTTGTAGTGTTTGCCAAGTCACATGA
  CCCCATTGAAGCCAGGTTGCGGTGTTTCTGTATGACAGATGACAAAGTAGATAAGACCCTTGAACAACAA
  GAAAACTTCTCTGAGGTGGCCAGGAGCAGGGATGTGGAGGTATTGGAAGGAAAACCTATTTATGTTGATT
  GTTTTGGCAACCTGGTTCCACTAACCAAGAGTGGCCAACATCATATATTCAGCTTTTTTGCCTTCAAAGA
  AAATAGACTTCCTCTCTTTGTCAAGGTTCGTGACACAACCCAGGAACCTTGCGGGCGACTCTCATTTATG
  AAGGAACCCAAATCCACAAGAGGACTGGTGCATCAAGCTATTTGCAACTTAAACATCACCCTGCCAATTT
  ATGCCAAGGAATCGGAGTCAGATCAAGAGCCGGAGGAAGAGATCGGTATGACATCCGAAAAAAATCCCCA
  AGATGAGCAGGAGCGGATGGAAGAAAGGCTGGCTTACATCGCTGATCACCTTGGCTTCAGCTGGACAGAA
  TTAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCACCAAATTCGAATCGAGAACCCCAACTCCCTTC
  AAGATCAGAGCCACGCACTGCTCAAGTACTGGCTGGAGAGGGATGGGAAGCATGCCACAGATACCATCCT
  CATCGAATGCCTCACCAAGATCAACAGGATGGACATTGTACATCTCCTGGAGACCAACACAGAGCCCCTC
  CAGGAGCGCATGGGCCGCAGCTATGCAGAAATAGAGCAGACCATTACGCTGGACCACAGTGAAGGATTTT
  CAGTCCTTCCAGACGAGCTCTGTGCTGCCAAGGAGAAGAAGGAGCAGGAAGCTTCCAAAGAAAGCGAGTC
  TAGCGACCACCCGCCCATGGTCTCCGAAGAAGACATATCTGTCGGTTATTCCACATTTCAGGATTGCCTC
  CCCAAAACTGAAGGGGACAGCCCAGCAGCAGCACTGTCTCCTCAAATGCACCAGGAGCCAGTTCAACAAG
  ATTTCTCAGGGAAAACGCAAGACCAGCAGGAATATTATGTAACAACACCAGGGGCAGAAGTGGAAGACCC
  TCAGAAGGCCACAGCCGTTCCTGACTCTCTCTGTAAGACTCCTGAGGACATCAGTACCCCTCCTGAGGGA
  ACAAAGCCTTGTCTCCAGACCCCGGTGACTAGCGAACGTGGTTCTCCGATTGTGCAAGAACCTGAGGAGG
  CTTCCGAGCCCAAAGAGGAGAGTTCTCCAAGGAAAACTAGCCTGGTCATTGTTGAGTCAACAGACGACCA
  GTCTCAGGTCTTTGAAAGACTGGATGGTGATGCCGCTTTTCAAAAGGGAGACGATATGCCTGACATACCC
  CCAGAGACGGTCACAGAGGAAGAATATGTTGATGAGAATGGACACACCGTGGTGAAGAAGGTTACCCGGA
  AAATCATTAGGCGGTACGTTTCCTCTGATGGCACAGAGAAGGAGGAGGTTACCATGCAGGGAATGCCTCA
  GGAGCCAGTCAACATTGAGGATGGGGACAATTATTCCAAAGTGATAAAGCGCGTGGTATTGAAGAGTGAC
  ACCCAGCAGTCAGAGGTGACTTTGTCTGAACCCAGCGTTTTGTCCAGTACCTCACAGTTTCAGGCCGAGC
  CAGTAGAAGGCCGTAGAGTCAGCAAAGTTGTTAAAACAACCATGGTACACGGAGAACGGATGGAGAAGAG
  TCTTGGGGACTCTAGCTTAGCCACTGACCTTCCTTCAGCCAAAGATGACTTTGAAGAGGACAACAATGAG
  TAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178655
• Insert Size: 3153 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178655.3, NP_848770.2
• RefSeq Size: 5790 bp
• RefSeq ORF: 3153 bp
• Locus ID: 109676
• UniProt ID: Q8C8R3
• Cytogenetics: 3 56.07 cM
• Gene Summary: Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions (PubMed:19109891). In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites (PubMed:12571597). Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:15262991). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (PubMed:19007774). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).[UniProtKB/Swiss-Prot Function]