Man2b1 (NM_010764) Mouse Untagged Clone

CAT#: MC223199

Man2b1 (untagged) - Mouse mannosidase 2, alpha B1 (Man2b1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Man2b1
• Synonyms: AW107687; LAMAN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223199 representing NM_010764
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCGGACCGCTCACCTCTGGTGTCCGTGCTGGTGGCGGGAACACCGGGTGGCTCTGGATGAGCT
  CCTGCAACCTAGGTTCGCCAGTCCTGCCTATTTCCTTTCTTTTTTGGCTGTTGCTGGCCGCGCCTGGCGC
  TCGGGCCGCGGGTTACAAGACATGTCCCCCAACGAAGCCAGGCATGCTCAATGTGCACCTGCTGCCTCAT
  ACACACGATGATGTAGGCTGGCTGAAGACAGTGGACCAATACTATTACGGCATCCTGAGTGATGTTCAGC
  ACGCATCTGTTCAGTACATCCTAGACTCAGTAGTCTCCTCTCTACTGGAGAAACCCACCCGTCGCTTCAT
  CTATGTGGAAATGGCTTTCTTCTCCCGTTGGTGGAAGCAGCAGACCAGTGCAACCCAGGACGCTGTACGG
  AACCTGGTGCGCCAGGGGCGCCTGGAGTTTGTCAATGGTGGCTGGGTGATGAACGACGAAGCAGCCACCC
  ACTATGGCGCGATTGTGGACCAGATGACACTTGGGCTACGCTTCCTGCAAGACACATTTGGTAGTGATGG
  ACTTCCCCGTGTGGCCTGGCATATTGACCCTTTCGGCCACTCTCGAGAACAGGCCTCCCTGTTTGCCCAG
  ATGGGTTTTGATGGCTTCTTCCTTGGGCGCATTGACTATCAAGATAAACTTAATCGGAAGAAAAAGCTGA
  GGATGGAGGAGCTGTGGAGAGCTAGTGACAGCTTGGAGCCCCCCGCTGCTGACCTCTTTACTGGTGTGCT
  CCCCAATAATTACAACCCCCCGAAATACCTGTGCTGGGATGTGCTATGTACAGACCCTCCCGTGGTGGAT
  AATCCCCGGAGCCCTGAGTTCAATGCAAAAACGTTGGTCAACTACTTCCTGAAGCTCGCTTCTTCCCAGA
  AAGGGTTTTACCGAACCAACCACACCGTGATGACCATGGGCTCAGACTTCCATTATGAGAATGCCAACAT
  GTGGTTCAAAAACATGGACAAGCTGATCCGCCTGGTCAACGCGCAGCAGGTAAACGGGAGCCTCGTCCAC
  GTTCTCTACTCCACCCCTACGTGTTACCTCTGGGAGCTGAACAAGGCCAATCTCACCTGGACAGTGAAGG
  AGGACGACTTCTTTCCTTATGCCGATGGCCCCCACATGTTCTGGACTGGCTATTTTTCCAGCAGGCCAGC
  CCTCAAGCGCTATGAGCGCCTCAGTTACAACTTCCTGCAGGTGTGTAACCAGCTGGAAGCACTGGTGGGT
  CCCGAAGCCAACGTGGGACCCTATGGCTCGGGAGACAGCGCACCTCTTCAGGAGGCAATGGCGGTGCTCC
  AACACCACGACGCTGTCAGTGGTACTGCAAGGCAGAATGTTGTAAATGACTACGCGAGGCAGCTGGCAGC
  AGGCTGGGGACCCTGTGAGGTTCTCGTGAGCAACGCTCTGGCGCGACTCAGCCATTACAAACAGAACTTC
  TCATTCTGTCGTGAGCTCAACATTAGCATCTGCCCAGTCAGCCAGACTTCGGAGCGTTTCCAGGTAACTC
  TTTACAACCCCCTGGGGCGAAAGGTGGATCAGATGGTTCGGCTGCCTGTCTATGAAGGCAACTTCATTGT
  GAAGGACCCTCATGATAAGAACATTTCCAGTAACGTGGTGATGGTTCCTAGCTATTACAGTGAGACTTAC
  CAATGGGAGCTGCTGTTCCCAGCCTCAGTGCCTGCCCTGGGCTTCAGCACCTACTCTGTGGCCAAAATGA
  GTGACCTTAACCACCAAGCCCACAACCTGCTGTCCAGGCCCAGGAAACACAAGTCCCATCATGTCTTAGT
  CATTGAAAATAAGTACATGCGGGCCACATTTGACTCTGGCACGGGGTTATTGATGAAGATTGAAAACTTG
  GAACAGAACCTCTCGCTCCCTGTGAGCCAAGGCTTCTTTTGGTATAATGCCAGTGTTGGTGATGAGGAGA
  GTTCTCAGGCCTCTGGGGCCTACATCTTCAGACCCAATGTCGGTAAACCGATACCTGTGAGCCGCTGGGC
  TCAGATCAGTTTGGTGAAGACAGCCTTGGTGCAGGAGGTACACCAGAATTTTTCAGCCTGGTGTTCCCAA
  GTGATTCGCCTGTACAAAGGACAGCGCCATTTGGAGCTGGAGTGGACGGTGGGACCAATCCCAGTGAGAG
  ATGACTGGGGGAAGGAAGTCATCAGCCGCTTTGACACCCCAATGAAAACCAAGGGACAGTTCTTCACGGA
  CAGCAACGGCCGGGAAATCCTGAAGAGGAGGGATGATTATCGACCCACCTGGACATTGAATCAGACTGAG
  CCGGTGGCTGGAAATTACTATCCTGTCAACACTCGAATTTATATCACGGATGGGCAAATGCAGCTGACCG
  TGTTGACCGACCGCTCCCAGGGGGGCAGCAGTCTGCAGGATGGCTCGTTGGAGCTCATGGTGCACCGTCG
  GCTGTTGGTAGATGATGATCGTGGAGTGTCGGAACCCTTGTTGGAGACAGATACCGGGGATAAGGTTCGA
  GGACGCCACCTTGTGCTTTTGAGTTCTGTCAGTGATGCCGCTGCAAGACACCGACTGCTGGCGGAACAAG
  AAGTCCTGGCCCCTCAGGTGGTGCTGTCTCTGGGTGGTAGCAGTCCCTATCACTCCCGGGCGACACCAAA
  AACGCAGTTTTCCGGACTTCGCCAGGAGCTACCTCCTCAGGTGCATTTGCTCACACTGGCCCGCTGGGGG
  CCAAAGATGCTGCTGCTGCGCTTGGAGCACCAGTTCGCTCTGAAAGAAGATTCGGATCGCAACCTGAGCT
  CCCCTGTAACCTTGAACGTTCAGAACCTGTTCCAGACCTTCACTATCAACTACCTGCAGGAGACCACACT
  GGCAGCCAACCAGCCCCTGTCCAGGGCTTCCAGGCTCAAGTGGATGACAAATACTGGTCCCACCTCCTAC
  CCTGAGCCTTCCAAGTTGGACCCTACCTCAGTCACACTGAAGCCTATGGAAATACGTACCTTCCTGGCCT
  CTGTACAATGGCAAGAACACAGGCCTGCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010764
• Insert Size: 3042 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_010764.2, NP_034894.2
• RefSeq Size: 4326 bp
• RefSeq ORF: 3042 bp
• Locus ID: 17159
• UniProt ID: O09159
• Cytogenetics: 8 41.55 cM
• Gene Summary: Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover.[UniProtKB/Swiss-Prot Function]