Llgl1 (NM_001159404) Mouse Untagged Clone

CAT#: MC223058

Llgl1 (untagged) - Mouse lethal giant larvae homolog 1 (Drosophila) (Llgl1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Llgl1
• Synonyms: AI325176; Lgl1; Llglh; Mgl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223058 representing NM_001159404
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAGTTTCGGTTCCGGCGGCAGGGCGCCGACCCGCAGCGCGAGAAGCTTAAGCAGGAGCTCTTCG
  CCTTCCACAAGACTGTGGAGCATGGCTTCCCCAATCAGCCCAGCGCCTTGGCCTTCGATCCTGAGCTCCG
  CATCATGGCCATCGGCACCAGATCTGGGGCCGTCAAGATCTATGGTGCACCCGGAGTGGAATTTACAGGC
  CTACATCGGGACGCAGCCACCGTCACCCAGATGCATTTCCTCCCTGGTCAGGGCCGCCTCCTGACCCTGC
  TAGATGACAGCAGCTTGCATCTCTGGGAGATCATCCATCATAATGGCTGCGCCCACCTGGAGGAAGGCCT
  CAGCTTCCACCCACCCAGCAGACCCAGTTTTGACAATGCCAGTTTCCCTGCCAGTCTAACACGTGTCACT
  GTGGTCCTGCTCGTAGCTGGCAATACAGCAGCCCTGGGAACTGAGAGTGGTAGCATATTCTTCCTGGATG
  TAGCCACCCTGGCACTGCTGGAGGGGCAGACTCTCAGCCCAGATGTGGTTCTGCGCAGTGTGCCAGATGA
  TTACCGGTGTGGAAAGGCCTTGGGCCCTGTGGAGTCACTCCAGGGACATCTGCAAGACCCCAGCAAGATC
  CTCATAGGCTACAGTCGGGGTTTACTGGTCATCTGGAGCCAGGCCACACAGTCTGTGGACAACGTTTTCC
  TAGGTAACCAGCAGCTGGAGAGCCTGTGTTGGGGCCGTGATGGCAGCAGCATTATCAGCTCACACAGTGA
  TGGCAGCTATGCCATCTGGTCCACAGACACTGGCAGCCCCCCAACGCTGCAGCCCACTGTAGTGACCACA
  CCCTACGGCCCCTTCCCCTGCAAGGCCATCAACAAGATTCTGTGGCGGAGCTGTGAGTCAGGAGACCACT
  TTATCATCTTCAGTGGTGGCATGCCTCGAGCCAGCTATGGTGACCGCCACTGTGTGAGTGTACTGCGAGC
  AGAGACACTGGTGACCCTGGACTTCACCTCTCGTGTCATTGACTTCTTCACGGTGCACAGCACACAGCCA
  GAGGATGAGTGTGACAACCCCCAGGCCTTAGCCGTGCTTCTGGAGGAGGAGCTGGTGGTGCTGGACCTGC
  AGACACCAGGCTGGCCAGCTGTGCCCGCCCCTTACCTGGCCCCACTGCATTCGTCAGCTATCACCTGCTC
  TGCCCATGTTGCCAATGTCCCCAGCAAGCTGTGGGCCCGCATTGTAAGTGCTGGTGAGCAGCAGAGCCCA
  CAGCCTGCCTCCAGTGCCTTGAGTTGGCCCATTACCGGGGGCCGGAACTTGGCCCAGGAACCCTCGCAGC
  GTGGGCTGCTGCTCACTGGCCATGAGGATGGCACTGTGCGGTTCTGGGACGCCTCTGGTGTGGCGCTAAG
  GCCACTCTACAAACTGAGCACAGCTGGCCTCTTCCAGACGGACTGTGAACATGCTGACAGCCTGGCCCAG
  GCTGTGGAGGATGACTGGCCGCCCTTCCGCAAGGTGGGCTGCTTTGATCCCTACAGTGATGACCCCCGGC
  TAGGAATCCAGAAGGTTGCGCTTTGCAAGTACACAGCCCAGATGGTGGTAGCTGGCACTGCAGGCCAGGT
  GCTGGTGCTGGAGCTCAGTGAGGTCCCAGCAGAGCATGCCGTCAGTGTGGCCAACGTGGATCTTCTTCAG
  GATCGCGAGGGCTTCACGTGGAAGGGTCACGAGCGGCTGAACCCACACACGGGGCTGCTGCCGTGGCCTG
  CCGGATTCCAGCCCCGCATGCTGATACAGTGCCTCCCACCCGCCGCTGTCACTGCTGTCACACTCCATGC
  TGAGTGGAGCCTCGTGGCCTTTGGTACCAGTCATGGCTTTGGCCTTTTTGACTACCAGCGCAAGAGCCCT
  GTGCTGGCTAGGTGCACCCTTCACCCCAATGATTCTTTGGCCATGGAGGGGCCACTGTCACGGGTGAAGT
  CCCTCAAGAAGTCACTGAGACAGTCATTCCGGCGAATCCGCAAGAGCCGTGTCTCAGGCAAAAAACGGAC
  TCCTGCTGCCAGTAGCAAGTTGCAGGAGGCCAATGCCCAGCTGGCCGAGCAGACCTGCCCACACGACCTG
  GAGATGACACCCGTGCAGCGCCGCATTGAGCCTCGGTCTGCTGACGACTCGCTCTCCGGTGTTGTACGCT
  GCCTCTACTTTGCTGACACGTTCCTTCGAGATGCGACCCACCACGGGCCCACCATGTGGGCGGGCACCAA
  CTCGGGCTCTGTGTTCGCCTATGCGCTGGAGGTTCCAGCAGCCACAGCAGGCGGAGAGAAGCGGCCTGAG
  CAGGCAGTGGAGGCGGTGCTGGGCAAGGAGGTGCAGCTAATGCACCGAGCACCTGTGGTGGCCATTGCTG
  TGCTGGATGGTCGTGGCCGGCCACTGCCTGAGCCCTATGAGGCTTCCCGGGACCTGGCCCAGGCGCCAGA
  CATGCAAGGCGGCCATGCTGTGCTCATTGCATCTGAGGAACAATTCAAGGTGTTCACACTACCCAAGGTG
  AGTGCTAAGACTAAATTCAAGCTTACAGCCCATGAAGGCTGTCGTGTGCGGAAGGTAGCCCTGGCTACAT
  TTGCCAGCGTGATGTCTGAGGACTATGCCGAGACCTGCCTTGCCTGCCTCACCAACCTGGGTGATGTCCA
  CGTCTTCTCGGTGCCTGGCCTGAGGCCTCAGGTGCACTACTCCTGTATCCGGAAGGAGGACATCAGTGGC
  ATCGCTTCCTGTGTCTTCACACGTCACGGCCAGGGCTTTTACTTGATTTCTCCATCGGAATTTGAGCGCT
  TCTCACTGAGTGCTCGCAACATCACAGAACCACTATGTTCTCTGGATATAAGCTGGCCCCAAAATGCCAC
  CCAGCCCAGGCTTCAAGAGTCACCCAAGCTGAGCCAGGCTAATGGGACCAGAGACATCATTCTGGCCCCA
  GAGAGCTGCGAAGGAAGCCCTAGCTCTGCCCACAGCAAGCGAGCTGATACCATGGAACCACCCGAGGCCG
  CTCTCTCGCCTGTGTCCATTGATTCAGCTGCTAGTGGGGATACCATGCTGGACACAACAGGGGATGTCAC
  CGTGGAATATGTGAAGGATTTTCTGGGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159404
• Insert Size: 3111 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159404.1, NP_001152876.1
• RefSeq Size: 4425 bp
• RefSeq ORF: 3111 bp
• Locus ID: 16897
• UniProt ID: Q80Y17
• Cytogenetics: 11 37.81 cM
• Gene Summary: Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells. Involved in axonogenesis through RAB10 activation thereby regulating vesicular membrane trafficking toward the axonal plasma membrane.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate splice pattern in the 3' coding region and an alternate splice site in the central coding region, compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus and lacks an internal two aa segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.