Prdm16 (NM_001177995) Mouse Untagged Clone

CAT#: MC223034

Prdm16 (untagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prdm16
• Synonyms: 5730557K01Rik; csp1; mel1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223034 representing NM_001177995
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGATCCAAGGCGAGGGCGAGGAAGCTAGCCAAAAGTGACGGTGACGTTGTAAATAATATGTATGAAC
  CTGACCCGGACCTGCTGGCCGGCCAGAGTGCCGAGGAGGAGACCGAAGACGGCATCCTGTCCCCCATCCC
  CATGGGGCCACCGTCCCCCTTCCCCACCAGCGAGGACTTCACTCCCAAGGAGGGCTCGCCCTATGAGGCT
  CCTGTCTACATTCCTGAAGACATTCCAATCCCACCAGACTTCGAGCTACGAGAGTCCTCCATACCAGGAG
  CTGGCCTGGGGATCTGGGCCAAGCGGAAGATGGAAATCGGGGAGAGGTTTGGCCCCTACGTGGTGACGCC
  CCGGGCCGCACTGAAGGAGGCCGACTTTGGATGGGAGCAGATGCTGACGGATACAGAGGTGTCATCCCAG
  GAGAGCTGCATCAAAAAGCAGATCTCTGAAGACTTGGGTAGCGAGAAGTTCTGCGTGGATGCCAATCAGG
  CGGGGTCTGGCAGCTGGCTCAAGTACATCCGTGTAGCGTGTTCCTGTGATGACCAAAACCTCGCCATGTG
  TCAGATCAACGAACAGATTTACTATAAAGTCATTAAGGACATCGAGCCTGGAGAGGAACTGTTGGTGCAT
  GTGAAAGAAGGTGCCTACTCCTTGGGTGTCATGGCCCCCAGCTTGGATGAGGACCCCACATTCCGCTGTG
  ATGAGTGTGATGAGCTCTTCCAGTGCAGGCTGGACCTGAGGCGCCACAAGAAGTACGCGTGCAGCTCTGC
  AGGAGCCCAGCTCTACGAGGGCCTAGGGGAGGAACTCAAGCCCGAGGGCCTTGGCGTGGGCAGCGACGGG
  CAAGCGCATGAGTGCAAGGATTGCGAGCGGATGTTCCCCAACAAGTACAGCTTGGAGCAACACATGATCG
  TCCACACGGAAGAGCGTGAGTACAAATGTGACCAGTGTCCCAAGGCCTTCAACTGGAAGTCCAACCTCAT
  CCGCCACCAGATGTCTCACGACAGTGGCAAGCGCTTCGAATGTGAAAACTGTGTCAAGGTGTTCACGGAC
  CCCAGCAACCTCCAGCGTCACATCCGCTCACAGCATGTCGGTGCCCGGGCCCATGCCTGCCCTGACTGTG
  GCAAGACCTTCGCCACATCCTCTGGCCTCAAACAGCACAAGCATATCCACAGCACGGTGAAGCCATTCAT
  ATGCGAGGTCTGCCACAAGTCCTACACGCAGTTCTCCAACCTGTGCCGGCACAAGCGGATGCACGCCGAC
  TGCAGGACGCAGATCAAGTGCAAGGACTGTGGGCAGATGTTCAGCACTACCTCCTCCCTCAACAAGCATC
  GGAGATTCTGCGAGGGCAAGAACCATTACACGCCTGGCAGCATCTTCACCCCAGGCCTGCCCTTGACCCC
  CAGCCCCATGATGGACAAGACAAAACCCTCCCCGACCCTCAACCACGGGGGCCTAGGCTTCAGCGAGTAC
  TTCCCCTCCAGACCTCATCCTGGGAGCCTGCCCTTCTCGGCTGCTCCTCCGGCCTTCCCCGCACTCACTC
  CGGGCTTCCCGGGCATCTTTCCTCCATCCCTGTACCCACGACCACCTCTGCTACCTCCCACGCCGCTGCT
  CAAGAGCCCCCTGAACCACGCGCAGGACGCCAAGCTACCCAGCCCGCTGGGAAACCCAGCCCTGCCCCTT
  GTCTCCGCGGTCAGCAATAGCAGCCAGGGTGCCACAGCGGCCACCGGGTCAGAGGAGAAATTTGATGGCC
  GCTTGGAAGACGCATATGCGGAGAAGGTCAAAAATAGGAGCCCTGACATGTCGGATGGCAGTGACTTTGA
  GGATATCAACACCACGACCGGGACAGACTTGGACACTACCACGGGCACGGGGTCAGACCTGGACAGCGAC
  CTGGACAGTGACAGAGACAAAGGCAAGGACAAGGGGAAGCCAGTGGAGAGCAAACCTGAGTTTGGGGGTG
  CATCTGTGCCCCCTGGGGCCATGAACAGTGTGGCCGAGGTACCGGCCTTCTACTCACAGCATTCCTTCTT
  CCCGCCACCCGAGGAACAGCTGCTGACGGCCTCGGGAGCTGCCGGCGACTCCATCAAGGCCATCGCGTCC
  ATCGCGGAGAAATACTTCGGTCCTGGCTTCATGAGCATGCAGGAGAAGAAGCTGGGCTCACTACCCTACC
  ACTCCGTGTTCCCCTTCCAGTTCCTGCCTAACTTTCCCCACTCCCTCTACCCCTTTACGGACCGAGCCCT
  CGCCCACAACTTGCTGGTCAAGGCTGAGCCAAAGTCACCCCGGGATGCCCTCAAGGTGGGCGGCCCCAGT
  GCGGAGTGCCCCTTCGACCTCACCACCAAACCAAAAGAGGCCAAACCCGCCCTGCTCGCACCCAAGGTCC
  CCCTCATCCCCTCATCTGGCGAGGAACAGCCACTGGACCTGAGCATCGGCAGCAGGGCCAGGGCAAGCCA
  GAACGGAGGTGGCCGTGAGCCGCGGAAGAACCACGTCTACGGTGAACGGAAGCCGGGGGTCAGCGAGGGG
  CTGCCTAAGGTGTGCCCAGCACAGCTGCCCCAGCAGCCCTCCTTGCATTATGCTAAGCCTTCACCGTTCT
  TCATGGATCCCATCTACAGGGTAGAAAAGCGGAAGGTGGCAGACCCTGTGGGAGTCCTGAAAGAGAAGTA
  CCTGCGGCCGTCCCCACTTCTGTTCCACCCCCAGATGTCAGCCATAGAAACCATGACGGAGAAGCTGGAG
  AGCTTTGCAGCCATGAAGGCCGACTCAGGCAGCTCCCTGCAGCCCCTGCCTCACCACCCGTTCAACTTCC
  GCTCCCCACCCCCAACGCTCTCGGATCCCATCCTCAGGAAGGGGAAGGAGAGATACACGTGCAGGTACTG
  TGGCAAGATCTTCCCCAGATCTGCAAATCTCACAAGACATCTGAGGACACACACAGGGGAGCAGCCATAC
  AGGTGCAAGTACTGTGACCGGTCATTCAGCATCTCCTCCAACCTCCAGCGGCACGTGAGGAACATCCACA
  ACAAAGAGAAGCCGTTCAAGTGCCATCTGTGCAACCGCTGCTTCGGGCAGCAGACCAACCTAGACCGGCA
  CCTGAAGAAGCACGAACACGAGGGCGCACCAGTGAGCCAGCACTCCGGGGTGCTCACGAACCACCTGGGC
  ACCAGCGCCTCCTCCCCCACCTCCGAGTCGGACAACCATGCACTTTTAGATGAGAAGGAAGATTCTTACT
  TCTCCGAGATCCGAAACTTCATCGCCAACAGCGAGATGAACCAGGCATCCACTCGAATGGACAAACGGCC
  TGAGATCCAAGACCTGGACAGCAACCCACCGTGTCCAGGCTCAGCCAGTGCAAAGCCAGAGGACGTAGAG
  GAGGAGGAAGAGGAGGAGCTGGAGGAAGAGGATGATGACAGCTTAGCCGGGAAGTCACAGGAGGACACGG
  TGTCCCCCACACCTGAGCCCCAAGGAGTCTATGAAGATGAAGAGGATGAGGAACCACCCAGCCTGACCAT
  GGGCTTTGACCATACCCGGAGGCATATGCAATGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_001177995
• Insert Size: 3534 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177995.1, NP_001171466.1
• RefSeq Size: 8433 bp
• RefSeq ORF: 3534 bp
• Locus ID: 70673
• Cytogenetics: 4 E2
• Gene Summary: Binds DNA and functions as a transcriptional regulator (PubMed:18483224). Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (PubMed:22939622). Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation (PubMed:22939622). Likely to be one of the primary histone methyltransferases along with MECOM/PRDM3 that direct cytoplasmic H3K9me1 methylation (PubMed:22939622). Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism (PubMed:17618855, PubMed:18483224). Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells (PubMed:18719582, PubMed:19641492). Functions as a repressor of TGF-beta signaling.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region and lacks an alternate exon in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.