Sik2 (NM_178710) Mouse Untagged Clone

CAT#: MC222719

Sik2 (untagged) - Mouse salt inducible kinase 2 (Sik2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sik2
• Synonyms: G630080D20Rik; Snf1lk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222719 representing NM_178710
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCAGTCCGGGTGGGGTTCTACGACATCGAGG
  GCACGCTGGGCAAGGGCAACTTTGCCGTGGTGAAGCTGGGGCGGCACCGGATCACCAAGACGGAGGTGGC
  TATAAAAATAATAGACAAGTCACAGCTGGATGCAGTAAACCTTGAGAAAATCTACCGAGAAGTACAGATA
  ATGAAAATGCTCGACCATCCTCACATCATTAAACTGTATCAGGTAATGGAGACCAAAAGTATGTTGTACC
  TTGTGACAGAATATGCCAAAAATGGAGAAATTTTTGATTATCTTGCTAATCATGGCCGATTAAATGAATC
  TGAAGCCAGGAGAAAATTCTGGCAAATTCTGTCTGCTGTTGATTATTGCCATGGCCGGAAGGTAGTGCAC
  AGAGACCTGAAGGCTGAAAATCTCCTGCTGGATAACAACATGAATATCAAAATAGCAGATTTCGGCTTTG
  GAAATTTCTTTAAAACTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCCCCTTATGCAGCCCCAGAAGT
  CTTTGAAGGGCAGCAGTATGAAGGACCACAGCTGGATATATGGAGCATGGGAGTTGTTCTTTATGTCCTT
  GTCTGTGGAGCTCTACCTTTTGATGGACCAACTCTCCCTATTTTGAGGCAGAGGGTTTTAGAAGGAAGAT
  TCCGGATTCCTTATTTCATGTCAGAAGATTGTGAACACCTCATTAGAAGGATGTTGGTCCTAGATCCTTC
  CAAACGGCTAAGCATAGCTCAAATCAAGGAGCACAAGTGGATGCTCATAGAGGTTCCTGTACAGAGGCCT
  ATTCTCTACCCACAAGAACAGGAAAACGAGCCGTCCATTGGAGAATTTAATGAGCAGGTTCTTCGACTGA
  TGCACAGCCTTGGGATTGATCAGCAGAAGACTGTTGAGTCTTTGCAAAACAAGAGCTATAATCACTTTGC
  TGCCATTTATTTCTTGTTGGTGGAACGTCTAAAATCACATAGGAGCAGTTTTCCTGTGGAGCAGAGACTT
  GATGGCCGCCAGCGTCGGCCTAGCACCATTGCTGAACAAACAGTTGCCAAGGCACAAACTGTGGGGCTGC
  CAGTGACCTTGCATCCACCGAACGTGAGACTGATGCGATCTACCCTCCTCCCACAGGCATCCAATGTGGA
  GGCCTTTTCATTTCCAACATCCAGCTGTCAAGCAGAAGCTGCCTTTATGGAGGAAGAGTGTGTCGACACT
  CCAAAGGTGAATGGCTGCCTGCTTGACCCTGTGCCCCCTGTCCTGGTGAGGAAGGGATGCCAGTCACTGC
  CCAGCAGTATGATGGAGACCTCCATTGATGAAGGCTTGGAGACAGAAGGAGAGGCTGAGGAAGACCCCAG
  TCAGGCCTTTGAAGCTTTCCAGGCCACACGCAGTGGGCAGCGACGGCACACTCTGTCAGAAGTGACTAAC
  CAATTGGTTGTGATGCCCGGGGCAGGGAAAATGTTCTCCATGAGTGATAACCCCTCCCTTGAGAGTGTGG
  ACTCTGAGTATGATATGGGGTCTGCCCAGAGGGACCTGAACTTTCTGGAAGACAGCCCTTCCTTGAAGGA
  CATCATGTTAGCCAATCAGCCGTCACCCCGCATGACATCTCCTTTCATAAGCCTCAGACCTGCCAACCCA
  GCCATGCAGGCTCTGAGCTCACAGAAGCGTGAGGCCCACAACCGGTCCCCCGTGAGCTTCCGAGAAGGCC
  GCAGAGCATCAGATACGTCCCTTACACAAGGAATTGTAGCATTTAGACAACATCTTCAGAATCTTGCTAG
  AACCAAAGGAATTCTGGAGTTGAACAAAGTACAATTGCTGTATGAACAAATGGGATCAAACGCAGACCCT
  ACCTTAACATCAACTGCTCCTCAGCTCCAAGACCTTTCGAGCAGTTGCCCTCAGGAGGAAATCTCCCAGC
  AGCAGGAAAGTGTCTCCAGCCTGTCTGCCAGCATGCACCCTCAGCTCTCACCACAGCAAAGCTTGGAAAC
  CCAGTACCTACAGCATCGACTCCAGAAGCCCAATCTTCTGCCAAAGGCCCAGAGTCCTTGTCCAGTGTAT
  TGTAAAGAGCCACCTCGGAGCCTGGAACAGCAGCTACAGGAGCATAGGCTCCAACAGAAGCGACTCTTCC
  TCCAGAAGCAGTCTCAGCTGCAAGCATATTTTAATCAGATGCAGATAGCAGAGAGCTCCTACCCTGGACC
  AAGTCAGCAGCTGGCTCTTCCCCACCAGGAGACTCCACTGACATCCCAGCAGCCCCCATCATTCAGCCTG
  ACCCAGGCCCTGAGTCCTGTCCTCGAGCCCTCTTCTGAGCAGATGCAATTTAGCTCTTTCCTCAGCCAAT
  ACCCAGAGATGCAGTTGCAGCCACTGCCCTCTACTCCCGGCCCCCAGGCTCCACCTCCCTTACCCTCACA
  ATTGCAGCAACATCAGCAGCCACCACCCCCACCACCACCCCCTCCACCACAGCAGCCAGGAGCTGCTCCA
  ACCTCCTTACAGTTCTCCTATCAGACTTGTGAGCTGCCAAGCACCACTTCTTCTGTACCAAACTATCCTG
  CTTCCTGTCACTATCCTGTGGATGGAGCCCAGCAGAGCAACCTCACAGGGGCGGACTGTCCCAGGAGCTC
  AGGACTTCAGGACACCGCATCTAGCTATGACCCACTGGCCCTCTCTGAGCTCCCTGGACTCTTTGATTGT
  GAAATGGTAGAAGCTGTGGATCCACAACACAATGGGGTTGTGAGCTGCTTAGCCCGGGAGACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178710
• Insert Size: 2796 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178710.3, NP_848825.2
• RefSeq Size: 3561 bp
• RefSeq ORF: 2796 bp
• Locus ID: 235344
• UniProt ID: Q8CFH6
• Cytogenetics: 9 A5.3
• Gene Summary: Phosphorylates 'Ser-789' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and inhibiting activity of TORCs, the CREB-specific coactivators, like CRTC2/TORC2 and CRTC3/TORC3 in response to cAMP signaling (PubMed:29211348).[UniProtKB/Swiss-Prot Function]