Ddhd1 (NM_001042719) Mouse Untagged Clone

CAT#: MC222606

Ddhd1 (untagged) - Mouse DDHD domain containing 1 (Ddhd1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ddhd1
• Synonyms: Mir5131; PA-PLA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222606 representing NM_001042719
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTACCCGGGCCGCGGCGCCCCACGGAGCCCCGAGCGTAACGGCCGCGGCGGCGGTGCCTGGGAGC
  TGGGCTCGGACGCGGCGGGCGTGTTCGGCGGCGGCGGCTGCTGCTTCGAGCACCAGCCGGGCGACAGCGT
  GCCGCTGTCGCTGCTGCGCGCCGAGCCGCTGCACCTGGCACCGGGCGCCGACGACCTGAGCCACCTGGCT
  CTGGACCCGTGCCTCAGCGACGAGACCTACGACTTCAGCTCGGCCGAGTCCGGCTCGTCGTTGCGCTACT
  ACAGCGAGGGCGAGAGCGCGGGCGGCGGCAGCTCTTCTTCGCCTCCGCCGCCGCTCGTCGCCCAGAACTC
  GGGGGGCGGCGGGGCGGCGGGAGGGGGCCCCGGGGACAGGAAGCGCGCCCGGCCCGGCGGCGCAGCCGCC
  CGGCACCGCTACGAGGTGGTGACGGAGCTGGGCCCCGAGGAGGTGCGCTGGTTCTACAAGGAAGACAAGA
  AGACCTGGAAGCCCTTCATCGGCTACGACTCTCTGCGCATCGAGCTCGCCTTCCGGACCCTGCTGCAGAC
  CACGGGCGCCCAGGCCCGGGCGGCGGGCCGCGATGGAGACCGGGTGTGTGGCCCAAGCTCCAGCTTCGGG
  GAGGAAGACGAGGAGGACAGCGCCTGCGGCTTCTGCCCGCGCGCGGCGGGGCCTGAGCCGGAGATGGAGG
  AGCTGGTGACCATCGAGCCGGTGTGCGTGCGCGGCGGCCTCTACGAGGTGGATGTCACTCAAGGAGAGTG
  CTACCCGGTGTACTGGAACCAAGCTGATAAAATACCAGTAATGCGTGGACAGTGGTTTATTGATGGGACG
  TGGCAACCTCTAGAAGAAGAAGAAAGTAATTTAATTGAGCAAGAACATCTCAGTTGTTTTAGGGGTCAGC
  AGATGCAAGAAAATTTTGATATTGAAGTGTCAAAATCCTTAGATGGAAAAGACGTTGTCTACCACCTCCC
  TCCCTTCTCCCTCCCTTCTCTGTTCAAATGGAGAGGATATAAGGAGAGCACAGATGCCACAGGTCTTAAG
  CGAAAGCGTTCCCAAGCTGTTCACAGTTTCAAGCTGAGTCGGAACCACGTGGACTGGCACAGCATGGATG
  AAGTCTATCTCTACAGTGACGCAACAACATCCAAAATTGCGAGAACCGTTACCCAAAAACTGGGCTTTTC
  TAAAGCCTCCAGCAGTGGGACCAGACTGCACAGAGGTTATGTAGAAGAAGCCACACTGGAAGACAAGCCG
  TCGCAGACTTCTCATATCGTGTTTGTTGTGCACGGCATTGGACAGAAGATGGACCAAGGAAGAATCATCA
  AAAACACAGCCATGATGAGAGAGGCTGCAAGGAAAATGGAAGAAAAGCATTTTTCCAACCATGCAACACA
  TGTTGAATTTCTGCCTGTTGAATGGCGATCAAAGCTTACTCTTGATGGAGACACAGTTGATTCCATCACT
  CCTGACAAAGTGCGAGGCCTGAGGGACATGCTCAACAGCAGCGCGATGGACATCATGTACTACACCAGCC
  CACTCTACAGGGATGAGCTAGTTAAAGGCCTTCAGCAAGAGCTGAATCGATTATATTCCCTTTTCTGTTC
  CCGGAATCCAGACTTTGAAGAGAAAGGGGGTAAAGTCTCAATAGTGTCCCATTCCTTGGGGTGTGTAATC
  ACTTATGACATCATGATGGGCTGGAATCCAGGTGGACTCTATGAGCAGCTGCTGCAGAAGGAGGAAGAGC
  TGCCCGACGAGCGGTGGATGAGCTATGAAGAGCGGCATCTTCTTGACGAGCTCTATATAACAAAGCGACG
  GCTGAGGGAAATAGAAGATCGGCTGCACGGATTGAAGGCGCCATCCATATCACAAACACCTGCCTTAAAA
  TTTAAGGTTGAAAATTTCTTCTGTATGGGATCTCCGCTAGCAGTTTTTTTGGCATTACGCGGCATCCGAC
  CAGGAAATTCTGGAAGTCAAGACCACATTTTACCTAGAGAGATCTGTAACCGCTTACTAAATATTTTTCA
  TCCTACGGACCCAGTGGCCTACAGATTAGAACCGTTGATTTTGAAACACTACAGCAACATTTCTCCAGTC
  CAGATCCACTGGTACAACACTTCAAACCCTCTACCTTATGAGCATATGAAGCCAAATTTTCTCAACCCAG
  CAAAAGAACCTACCTCAGTTTCAGACAGTGAGAACATAGCAGCTATCCCAAGCCCTGTGACCTCCCCCGT
  CTTATCTCGGCGCCACTATGGAGAATCTATAACTAACATTGGCAAAGCAAGCATATTGGGGGCTGCTAGC
  ATTGGAAAGGGCCTTGGAGGAATGTTGTTCTCAAGATTTGGACGTTCTTCAGCATCACAGCCGTCTGAGC
  CATCTAAAGACTCACTGGAAGATGATAAGAAGCCTTCAGCTTCACCCTCTACCACCACCGTGGCAACGCA
  GACCCTGCCGCACAGTGGCTCCGGCTTCCTCGACTCCGCATATTTCAGACTTCAAGAATCGTTCTTTTAT
  CTCCCACAACTTCTTTTTCCGGAAAATGTAATGCAGAGTAAAGATGATAGCCTCGTGGAACTGGAACACA
  GGATCGACTTTGAACTCAGAGAAGGCCTTGTGGAGAGCCGCTATTGGTCAGCCGTCACGTCGCACACTGC
  CTATTGGTCATCCTTGGATGTCGCTCTTTTTCTTTTAACATTCATGTACAAACACGAGCATGATACCGAG
  GCCAAGCCCAGCCTAGGTTCACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042719
• Insert Size: 2757 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042719.2, NP_001036184.1
• RefSeq Size: 5201 bp
• RefSeq ORF: 2757 bp
• Locus ID: 114874
• UniProt ID: Q80YA3
• Cytogenetics: 14 C1
• Gene Summary: Phospholipase that hydrolyzes phosphatidic acid, including 1,2-dioleoyl-sn-phosphatidic acid. The different isoforms may change the substrate specificity (By similarity). Required for the organization of the endoplasmic reticulum exit sites (ERES), also known as transitional endoplasmic reticulum (tER) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3).