Cul5 (NM_027807) Mouse Untagged Clone

CAT#: MC222170

Cul5 (untagged) - Mouse cullin 5 (Cul5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cul5
• Synonyms: 4921514I20Rik; 8430423K24Rik; AI852817; C030032G03Rik; C330021I08Rik; VACM-1; VACM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222170 representing NM_027807
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTCTCTCGCGTGGGCAGGCCGGGGTGACCATGTAGCTGGAAAGCCCGAGGAAGCACGGCTGCCCG
  GGACGAGCTCGGCGCTGACGGCACGCCGCTCCGGCGTCCCCCGCATCCCCCGCCGCGGCCTGCGGGGTCC
  TGCTGGGAACCCCGGCCTCTCGAGGAGGCCTGGCCCCGAGCGCCGCCAAGTCTCGCCCCGTCTCGCGAGA
  GTCCAAGTTGAGAACATGGCGACGTCTAATCTGTTAAAGAATAAAGGTTCTCTCCAGTTTGAAGACAAGT
  GGGACTTCATGCATCCAATTGTTTTGAAGCTTTTACGCCAGGAATCTGTAACAAAACAGCAGTGGTTTGA
  TCTATTTTCGGATGTACATGCTGTCTGTCTCTGGGATGATAAAGGCTCATCAAAAATTCATCAGGCTTTA
  AAAGAAGATATTCTTGAGTTTATTAAGCAAGCACAGGCTCGTGTACTGAGCCATCAAGATGACACAGCTT
  TGCTGAAGGCATATATTGTTGAATGGCGGAAATTCTTCACACAGTGTGATATTTTACCAAAACCTTTTTG
  TCAATTAGAGGTGACTCTATTGGGTAAACAAAGCAGCAATAAAAAATCAAATATGGAAGACAGTATTGTT
  CGAAAGCTCATGCTTGATACGTGGAATGAGTCGATTTTTTCAAATATAAAGAACAGACTCCAGGACAGTG
  CAATGAAGCTGGTGCATGCTGAGAGATTAGGGGAAGCTTTTGATTCCCAGCTGGTCATCGGGGTGCGAGA
  GTCCTATGTTAATCTTTGCTCCAACCCCGAGGACAAGCTTCAGATCTATAGGGATAATTTTGAGAAGGCA
  TACTTGGACTCAACAGAGAGGTTTTATAGAACACAGGCACCCTCATATTTACAGCAAAATGGTGTGCAGA
  ATTACATGAAATATGCAGATGCTAAATTAAAAGAAGAAGAAAAAAGAGCACTCCGATACTTAGAAACACG
  ACGAGAGTGTAACTCTGTGGAAGCACTCATGGAATGCTGTGTAAATGCGCTGGTGACCTCCTTTAAAGAG
  ACTATTTTAGCAGAATGCCAAGGCATGATCAAGCGAAATGAAACTGAAAAGTTACATTTGATGTTTTCCT
  TGATGGACAAAGTTCCTAATGGGATAGAGCCGATGTTGAAGGACTTGGAGGAGCATATTATAAGTGCGGG
  CCTAGCAGACATGGTGGCCGCAGCTGAAACCATCACTACTGACTCTGAGAAGTATGTGGAGCAATTACTT
  ACACTGTTTAATAGATTCAGTAAACTGGTCAAAGAAGCTTTTCAGGATGATCCTCGTTTCCTTACTGCAA
  GAGATAAGGCATATAAAGCAGTTGTTAATGATGCTACTATATTTAAACTTGAATTGCCTTTGAAGCAAAA
  AGGAGTGGGGTTGAAAACTCAGCCTGAGTCAAAATGCCCCGAGTTGCTTGCCAATTACTGTGACATGTTG
  TTAAGGAAAACGCCATTAAGCAAAAAACTAACATCTGAGGAGATTGAAGCAAAGCTTAAAGAAGTGCTCT
  TGGTACTTAAATATGTACAAAACAAAGATGTTTTTATGAGGTATCACAAAGCTCATCTTACCCGACGTCT
  CATATTGGACATCTCTGCTGATAGTGAGATTGAAGAAAACATGGTAGAGTGGCTAAGAGAAGTTGGTATG
  CCAGCAGATTATGTGAACAAGCTTGCTAGAATGTTTCAGGACATAAAAGTATCTGAAGACTTGAACCAAG
  CTTTTAAGGAAATGCACAAAAATAATAAGTTGGCATTACCAGCTGATTCCGTTAATATAAAGATTTTGAA
  TGCTGGTGCTTGGTCTAGAAGCTCCGAGAAAGTCTTTGTCTCACTTCCTACTGAACTGGAGGATTTGATA
  CCTGAAGTAGAAGAATTTTACAAAAAAAATCACAGTGGTAGAAAATTACACTGGCACCATCTCATGTCAA
  ATGGAATTATAACATTTAAAAATGAAGTAGGTCAGTATGATTTGGAAGTAACCACGTTTCAGTTGGCTGT
  GTTGTTTGCATGGAACCAAAGGCCTAGAGAGAAAATCAGCTTTGAAAATCTAAAACTTGCAACGGAACTC
  CCAGATGCTGAACTTAGAAGGACTTTATGGTCTTTAGTAGCTTTTCCCAAGCTCAAACGGCAAGTTTTGT
  TGTATGACCCTCAAGTCAACTCACCCAAAGATTTTACAGAAGGCACCCTCTTCTCAGTGAACCAGGACTT
  CAGTCTCATAAAAAATGCAAAAGTACAGAAAAGGGGGAAAATCAATTTGATTGGACGCTTGCAGCTCACT
  ACAGAACGAATGAGAGAAGAAGAAAATGAAGGGATAGTCCAACTAAGAATATTAAGAACCCAGGAAGCCA
  TCATACAAATAATGAAAATGAGAAAGAAAATTAGCAATGCCCAGCTGCAGACTGAATTAGTAGAAATTCT
  GAAAAACATGTTCCTGCCTCAGAAGAAGATGATAAAGGAGCAGATGGAGTGGCTGATTGAACACAGGTAC
  ATCCGGAGGGACGAGGCCGACATCAACACCTTCATCTACATGGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027807
• Insert Size: 2568 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027807.3, NP_082083.2
• RefSeq Size: 6023 bp
• RefSeq ORF: 2568 bp
• Locus ID: 75717
• UniProt ID: Q9D5V5
• Cytogenetics: 9 A5.3
• Gene Summary: Core component of multiple SCF-like ECS (Elongin BC-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.