Pfkm (NM_001163488) Mouse Untagged Clone
CAT#: MC221560
Pfkm (untagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 3, (10ug)
"NM_001163488" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Pfkm |
Synonyms | AI131669; ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka; Pfkx |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC221560 representing NM_001163488
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCATGAAGAGCATCATGCAGCCAAAACCCTGGGGATCGGCAAGGCCATCGCCGTGTTGACCTCTG GTGGAGATGCCCAAGGTATGAATGCTGCCGTCAGGGCTGTGGTCCGAGTTGGTATCTTCACGGGCGCCCG CGTCTTCTTTGTCCATGAGGGTTACCAAGGCCTGGTGGACGGTGGCGAGCACATCAGGGAGGCCACGTGG GAGAGCGTGTCTATGATGCTTCAGCTGGGTGGCACAGTGATTGGAAGTGCCCGATGCAAGGACTTCCGAG AGCGAGAAGGACGACTCCGGGCCGCCCACAACCTGGTGAAGCGGGGGATCACCAATCTGTGTGTCATCGG AGGCGATGGCAGCCTCACTGGGGCTGACACTTTCCGTTCAGAGTGGAGCGACTTGCTGAATGATCTCCAG AAAGACGGGAAGATCACAGCCGAGGAGGCTACAAAGTCCAGCTACCTGAACATCGTGGGCCTGGTTGGCT CAATCGACAATGACTTCTGTGGCACTGATATGACCATTGGTACCGACTCTGCCCTGCACAGGATTGTGGA GATCGTAGACGCCATCACCACCACGGCTCAGAGCCACCAGAGGACGTTTGTGTTAGAAGTGATGGGCCGC CACTGTGGATACCTGGCCCTTGTCACCTCTCTGTCCTGTGGGGCCGACTGGGTTTTTATTCCTGAGTGTC CGCCAGACGATGACTGGGAAGAACACCTTTGTCGCCGGCTCAGTGAGACAAGGACCCGTGGCTCTCGTCT CAACATCATCATTGTTGCTGAAGGTGCCATCGACAAGAACGGGAAGCCAATCACCTCAGAAGACATCAAG AACCTGGTGGTGAAGCGCCTTGGATATGACACCCGGGTCACTGTTCTGGGACATGTACAGCGGGGTGGGA CACCATCAGCCTTTGACCGGATCCTGGGCAGCAGGATGGGTGTGGAAGCAGTGATGGCACTTTTGGAGGG GACCCCAGACACCCCAGCCTGTGTGGTGAGCCTCTCTGGTAACCAGGCTGTGCGTTTGCCCCTCATGGAG TGCGTGCAGGTGACCAAAGACGTGACCAAGGCTATGGATGAGAAGAGATTTGATGAAGCCATTAAGCTGA GAGGCCGGAGCTTCATGAACAACTGGGAGGTATACAAGCTTCTAGCTCATGTCAGACCCCCAGTCTCTAA GGGTGGGTTGCACACAGTGGCCGTGATGAATGTGGGGGCCCCAGCTGCTGGCATGAATGCCGCTGTTCGC TCTACCGTGAGGATTGGCCTTATCCAGGGCAACCGGGTGCTGGTCGTACATGATGGCTTTGAGGGTCTGG CCAAAGGTCAGATTGAGGAGGCTGGCTGGAGCTATGTTGGAGGCTGGACTGGTCAAGGTGGTTCCAAACT TGGTACTAAAAGGACTCTCCCCAAGAAGAACCTGGAACAGATCAGTGCCAACATAACCAAGTTTAACATC CAGGGCCTTGTCATCATTGGGGGCTTTGAGGCTTACACAGGGGGCTTGGAGCTGATGGAAGGCAGGAAGC AGTTTGATGAGCTCTGCATCCCGTTTGTGGTCATTCCGGCCACGGTTTCCAATAACGTCCCTGGGTCAGA CTTCAGCATCGGGGCTGACACAGCACTGAACACCATCTGCACGACTTGTGACCGAATCAAGCAGTCTGCA GCAGGCACCAAACGTCGGGTGTTTATCATCGAGACTATGGGTGGCTACTGTGGCTACCTGGCCACCATGG CAGGGCTGGCCGCTGGGGCTGATGCTGCCTACATTTTTGAGGAACCCTTCACCATTCGCGATCTCCAGGT GAATGTTGAACATCTGGTGCAGAAGATGAAAACAACTGTGAAGAGAGGCCTGGTGCTGAGGAATGAGAAA TGCAATGAGAACTACACTACTGACTTCATTTTCAACCTGTACTCTGAGGAGGGGAAGGGCATCTTTGATA GCAGGAAGAACGTGCTTGGCCACATGCAGCAGGGTGGAAGCCCAACTCCCTTTGACAGGAATTTTGCCAC TAAGATGGGTGCTAAGGCTATGAACTGGATGTCTGGGAAAATCAAGGAGAGTTACCGTAATGGACGGATC TTTGCCAACACCCCTGACTCAGGCTGCGTTCTGGGGATGCGTAAGAGGGCCCTGGTCTTTCAGCCAGTAA CTGAGCTGAAGGACCAGACAGACTTTGAACACCGAATCCCCAAAGAACAGTGGTGGCTGAAGCTGAGGCC AATCCTCAAAATCCTAGCCAAGTACGAGATTGATCTGGACACCTCCGACCACGCCCACCTGGAGCACATT TCAAGGAAACGGTCTGGAGAAGCCGCCGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001163488 |
Insert Size | 2343 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163488.1, NP_001156960.1 |
RefSeq Size | 2830 bp |
RefSeq ORF | 2343 bp |
Locus ID | 18642 |
UniProt ID | P47857 |
Cytogenetics | 15 F1 |
Gene Summary | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternative 5' non-coding exon compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG221898 | Pfkm (tGFP-tagged) - Mouse phosphofructokinase muscle (Pfkm) transcript variant 3, (10ug) |
USD 915.00 |
|
MR221898 | Pfkm (Myc-DDK-tagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 3 |
USD 715.00 |
|
MR221898L3 | Lenti ORF clone of Pfkm (Myc-DDK-tagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 3 |
USD 1,015.00 |
|
MR221898L4 | Lenti ORF clone of Pfkm (mGFP-tagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 3 |
USD 1,015.00 |
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