Kcnn3 (NM_080466) Mouse Untagged Clone

CAT#: MC221191

Kcnn3 (untagged) - Mouse potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3 (Kcnn3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnn3
• Synonyms: KCa2.3; SK3; SKCA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221191 representing NM_080466
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACTTCTGGGCACTTCCATGACTCGGGGGTGGGGGACCTGGATGAAGACCCCAAGTGTCCCTGTC
  CATCTTCTGGGGACGAGCAACAGCAGCAGCAGCAACCGCCACCACCGCCAGCGCCACCAGCAGTCCCCCA
  GCAGCCTCCGGGACCCTTGCTGCAGCCTCAGCCTCCGCAGCCTCAGCAGCAACAGTCGCAGCAGCAGCAG
  CAGCAGCAGTCGCAGCAGCAGCAGCAGCAGGCTCCACTGCACCCCCTGCCTCAGCTTGCCCAACTCCAGA
  GCCAGCTTGTCCATCCTGGTCTGTTGCACTCTTCTCCCACGGCGTTCAGGGCCCCCACTTCAGCCAACTC
  TACCGCCATCCTCCACCCTTCCTCCAGGCAAGGCAGCCAGCTAAATCTCAATGACCACTTGCTTGGCCAC
  TCTCCAAGTTCCACAGCCACAAGTGGGCCCGGTGGAGGCAGCCGGCACCGGCAGGCCAGCCCCCTGGTGC
  ACCGGCGGGACAGCAATCCCTTCACGGAGATAGCTATGAGCTCCTGCAAATACAGCGGTGGGGTCATGAA
  GCCCCTCAGCCGCCTCAGCGCCTCTCGGAGAAACCTTATCGAGGCTGAGCCTGAGGGCCAACCCCTCCAG
  CTCTTCAGTCCCAGCAACCCCCCAGAGATTATCATCTCCTCCAGGGAGGATAACCATGCCCACCAGACTC
  TGCTCCATCACCCCAACGCTACCCACAACCACCAGCATGCCGGCACCACGGCCGGCAGCACCACCTTCCC
  CAAAGCCAACAAGCGGAAAAACCAAAACATTGGCTATAAGCTGGGACACAGGAGGGCCCTGTTTGAAAAG
  AGAAAGCGACTGAGTGACTATGCTCTGATTTTTGGGATGTTTGGAATTGTTGTTATGGTGATAGAGACCG
  AACTGTCTTGGGGTTTGTACTCAAAGGATTCCATGTTTTCGTTGGCCCTGAAATGCCTTATCAGTTTATC
  CACCGTCATCCTGCTTGGTTTGATCATCGCCTATCACACAAGGGAAGTACAGCTCTTTGTGATCGACAAT
  GGTGCAGATGACTGGCGGATAGCCATGACCTACGAGCGTATCCTCTACATCAGCCTGGAGATGCTGGTGT
  GCGCCATCCACCCCATCCCTGGAGAGTACAAGTTCTTCTGGACTGCACGCCTGGCCTTCTCCTATACCCC
  ATCTCGGGCAGAGGCTGACGTGGACATTATTCTGTCCATCCCCATGTTCCTGCGCCTGTACCTGATCGCC
  CGGGTCATGCTGCTCCATAGCAAACTCTTCACTGATGCCTCCTCCCGAAGCATCGGGGCCCTCAACAAGA
  TCAACTTCAACACCCGATTCGTCATGAAGACGCTCATGACCATCTGCCCTGGCACGGTGCTGCTGGTGTT
  CAGCATCTCTCTGTGGATCATCGCTGCCTGGACTGTGCGAGTCTGTGAAAGGTACCATGATCAGCAGGAT
  GTAACTAGTAACTTTCTGGGTGCCATGTGGCTCATCTCCATCACGTTCCTTTCCATTGGCTATGGGGACA
  TGGTGCCCCACACATACTGTGGGAAAGGTGTCTGTCTTCTCACCGGCATCATGGGTGCAGGCTGCACTGC
  CCTTGTGGTAGCTGTGGTTGCCCGAAAGCTCGAACTCACCAAAGCAGAGAAGCATGTGCACAACTTCATG
  ATGGACACTCAGCTCACCAAACGGATCAAGAATGCCGCCGCCAATGTCCTCCGGGAAACGTGGCTAATCT
  ATAAACATACAAAGCTGCTAAAGAAGATTGACCATGCCAAAGTCAGGAAACACCAGAGGAAGTTCCTCCA
  AGCTATCCACCAACTGCGGGGTGTCAAGATGGAACAAAGGAAGCTGAGTGACCAAGCCAACACCCTGGTG
  GACCTTTCCAAGATGCAGAACGTCATGTATGACTTAATCACGGAGCTCAATGACCGGAGTGAAGATCTAG
  AAAAGCAGATTGGCAGCCTGGAGTCCAAGCTGGAGCACCTCACAGCCAGTTTCAATTCCCTGCCCCTGCT
  CATCGCAGATACCCTGCGCCAGCAGCAGCAGCAGCTGCTCACTGCCTTCGTGGAGGCCCGGGGCATCAGT
  GTGGCCGTGGGAACTAGCCACGCCCCTCCCTCTGACAGCCCTATCGGGATCAGCTCCACCTCTTTCCCAA
  CCCCATACACAAGTTCAAGCAGTTGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_080466
• Insert Size: 2199 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080466.2, NP_536714.2
• RefSeq Size: 7617 bp
• RefSeq ORF: 2199 bp
• Locus ID: 140493
• UniProt ID: P58391
• Cytogenetics: 3 F1
• Gene Summary: Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin (By similarity).[UniProtKB/Swiss-Prot Function]