Polh (NM_030715) Mouse Untagged Clone

CAT#: MC220523

Polh (untagged) - Mouse polymerase (DNA directed), eta (RAD 30 related) (Polh), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Polh
• Synonyms: RAD30A; XPV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220523 representing NM_030715
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTGGGCAGAATCGAGTGGTTGCTCTTGTAGACATGGACTGCTTTTTTGTACAAGTGGAACAGC
  GGCAAAATCCTCATTTGAGGAATAAACCTTGTGCAGTTGTACAGTACAAATCATGGAAAGGTGGTGGAAT
  TATTGCAGTTAGCTATGAAGCCCGAGCATTTGGTGTCACTAGAAACATGTGGGCGGATGATGCTAAGAAG
  TTATGTCCAGATCTTCTCCTGGCACAAGTTCGTGAGTCCCGTGGAAAAGCTAACCTCACCAAGTACCGGG
  AAGCTAGTGTTGAAGTGATGGAGATAATGTCTTATTTTGCTGTGATTGAACGTGCCAGCATCGATGAGGC
  TTATATAGATTTGACCAGTGCTGTACAAGAAAGACTTCAAAAGTTACAAGGTCAGCCTATCTCAGCAGAT
  TTGCTGCCAAGCACTTACATTGAAGGGTTGCCCCGAGGCCCTACAGTAGAAGAGACTGTTCAGAAAGAGG
  CTATTCGGAAACAAGGCTTGCTTCAGTGGCTTGATTCTCTTCAGAGTGATGATCCCACCTCTCCAGACCT
  CAGGCTGACTGTGGGAGCCATGATTGTGGAAGAAATGAGAGCAGCCATAGAGAGTAAGACTGGCTTCCAG
  TGTTCAGCTGGAATCTCACACAATAAGGTCCTGGCAAAACTTGCTTGTGGACTAAATAAGCCCAACCGCC
  AAACCCTGGTCTCACATGGGTCAGTGCCACAGCTCTTCAGCCAGATGCCAATCCGTAAAATCCGAAGTCT
  TGGAGGAAAGCTAGGGGCTTCTGTCATTGAAGTCCTTGGGATAGAATACATGGGTGACCTGACCCAGTTC
  ACTGAATCCCAGCTCCAGAGTCATTTTGGAGAGAAGAATGGGTCTTGGCTGTATGCCATGTGTCGAGGTA
  TTGAACACGATCCAGTTAAACCCAGGCAGTTACCTAAAACTATTGGCTGCAGCAAGAACTTCCCAGGGAA
  AACAGCTCTGGCTACTCGGGAGCAGGTACAGTGGTGGCTTTTGCAGTTAGCTCTGGAACTTGAGGAGAGG
  CTAACCAAAGATCGAAATGATAATGACCGCGTGGCCACTCAGCTGGTCGTCAGTATTCGTTTTCAGGGAG
  ACAGGCGTCTCAGCAGCCTGCGGCGGTGCTGTGCCCTCCCTCGCTATGACGCTCACAAGATGAGCCAGGA
  CGCATTTGCTGCCATCAGGAACTGTAATACATCTGGAATCCAAACTGAGTGGTCCCCTCCTCTCACAATG
  CTCTTCCTCTGCGCTACCAAGTTCTCTGCGGCTGCCCCTCCAGCTTGCACGGATATCACTGCGTTCCTGA
  GTAGCGACTCAAGTTGTCAGCCAAAGGTGCCAATTGCCAGCTCTGAGACTAGGACCCAGGGGTCTGGCCC
  AGCTGTACCAACCTCTAAAGAGGCAGCCACATCTCTAGCGTCTTTTTTTCAAAAAGCTGCCAAAAAGCAG
  AGAATGAAAGAGACTTCCTTTGTACCTCTTAACACAGCCACAGAGAAGTTATCATCCAAGCCTTCACTTG
  TGTTCCAAAGCAGCCAAACCACAGGAAGTCAGTCTTTCTTTAAGCAGAAGAGCCTGCTGCTCCAGCACAC
  ACAGCTTAGTAACTCTGCGGCTCCTGACCCTCCACAGGCCAGCCCTGCAGCGCAACCAAGCTGTCTGCCA
  GCAGAGTGTGTAGACAGTGGTCCTGATGATGGAGCGGTGAAGCCAGTATCCTCTAAAGCAGTGTCTACAG
  AAATGAATGTGGCTGGGGACAGTCCAAACGTGCTCGATTCTCCAGCTTACAACTCTCAGGAGGTGACCCA
  AAGGGCCACTGAGGACCAAGTGCTCTGTGAGAAGTGTGACTCCCTGGTTCCAGTGTGGGACATGCCAGAA
  CACACGGACTACCATTTTGCATTGGAGTTGCAGAAGTCTTTTTTGCAGCCTTGTACTTCAAAACCCCAGG
  CTATTCCTGCCGTGTCTCCTCAGGGCAAAAGAAATCCCAAGAGTCCCTCGGCTTCCAGTAGTAAGCGCCT
  TAGGCCCCATGGCATGCAGACGCTGGAATCGTTTTTTAAGCCACTGACACATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030715
• Insert Size: 2085 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030715.3, NP_109640.1
• RefSeq Size: 2580 bp
• RefSeq ORF: 2085 bp
• Locus ID: 80905
• UniProt ID: Q9JJN0
• Cytogenetics: 17 C
• Gene Summary: DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10871396). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (By similarity). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10871396). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis. Targets POLI to replication foci (By similarity).[UniProtKB/Swiss-Prot Function]