Add1 (NM_001102444) Mouse Untagged Clone

CAT#: MC220230

Add1 (untagged) - Mouse adducin 1 (alpha) (Add1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Add1
• Synonyms: AI256389
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220230 representing NM_001102444
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGTGACACTCGGGCCGCAGTGGTGACCTCACCACCTCCAACCACAGCCCCTCACAAGGAGAGGT
  ATTTTGACAGAGTCGATGAGAACAACCCAGAATATTTGCGGGAGAGGAACATGGCACCAGACCTTCGTCA
  GGACTTCAATATGATGGAGCAGAAGAAGAGGGTGTCTATGATTCTACAGAGTCCTGCCTTCTGTGAAGAG
  CTGGAGTCTATGATACAGGAACAGTTTAAGAAGGGCAAGAACCCCACAGGCCTGCTGGCATTGCAGCAGA
  TTGCAGATTTCATGACTGCGAGTGTACCAAATGTCTACCCGGCAGCTCCTCAAGGAGGAATGGCCGCCCT
  GAACATGAGTCTTGGTATGGTCACTCCTGTGAATGACCTTAGAGGGTCGGATTCTATCGCATATGACAAA
  GGGGAGAAGTTACTGCGGTGTAAACTGGCAGCATTTTACAGACTGGCAGATCTCTTTGGCTGGTCCCAGC
  TTATCTACAATCATATCACAACCAGAGTGAACTCTGAGCAGGAACACTTCCTCATCGTACCTTTTGGACT
  TCTCTACAGTGAAGTGACTGCATCCAGCTTGGTTAAGGTCAATCTACAAGGAGATATAGTAGACCGTGGA
  AGTACTAACCTAGGGGTGAATCAGGCGGGCTTCACTCTGCACTCGGCAGTGTATGCTGCGCGACCAGATG
  CCAAGTGTATTGTGCACATCCACACCCCAGCGGGGGCAGCGGTCTCTGCGATGAAGTGTGGACTCTTGCC
  TATCTCCCCGGAGGCACTTTCCCTTGGAGATGTTGCTTATCATGACTATCATGGCATTCTGGTTGATGAA
  GAGGAAAAAATTTTGATTCAGAAAAATCTGGGCCCTAAAAGCAAGGTCCTCATTCTGCGGAATCACGGAC
  TCGTGTCAGTTGGAGAGAGCGTGGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCATGTGAGAT
  CCAGGTTCGGACTCTGGCAAGTGCTGGAGGACCAGACAACTTAGTTCTGCTGGATCCTGGGAAGTACAAA
  GCCAAATCCCGTTCTCCAGGGACTCCAGCAGGGGAAGGCTCTGGATCACCTCCCAAGTGGCAGATTGGGG
  AGCAGGAATTTGAAGCTCTTATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATA
  CCCTGCTCTGAGAGAGAGATCTAAAAAGTACAGCGATGTGGAAGTCCCTGCCAGTGTCACAGGCCACTCC
  TTTGCTAGTGATGGCGATTCGGGCACTTGCTCGCCGCTCAGGCACAGTTTTCAGAAGCAGCAGCGAGAGA
  AGACAAGATGGCTGCACTCTGGCCGGGGTGATGACGCTTCTGAGGAGGGGCAGAACGGAAGCAGTCCCAA
  GTCGAAGACTAAGGTGTGGACGAACATTACACACGATCACGTGAAACCCTTGCTGCAGTCTCTCTCGTCC
  GGTGTCTGCGTGCCAAGCTGTATTACCAACTGCTTGTGGACTAAAGAGGATGGACATAGAACTTCCACCT
  CTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGAT
  TCGAGAGCAGAACTTACAGGACATTAAGACGGCAGGACCTCAGTCCCAGGTTTTGTGTGGTGTGATGATG
  GACAGAAGCCTTGTTCAGGGGGAGCTGGTGACGGCCTCCAAAGCCATCATTGAGAAGGAATACCAGCCCC
  ATGTAATTGTGAGCACCACAGGTCCCAACCCCTTCAACACGCTGACAGACCGTGAACTGGAAGAATACCG
  ACGAGAGGTGGAGCGGAAGCAGAAGGGCTCTGAAGAGAACCTGGATGAGACAAGAGAGCAGAAAGAGAAG
  AGTCCTCCAGACCAGTCAGCTGTCCCCAACACTCCTCCCAGTACTCCTGTCAAGCTTGAAGAAGCAGGAG
  ACGGATGTGCTAAAGAGTACCTGTTACCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001102444
• Insert Size: 1992 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001102444.1, NP_001095914.1
• RefSeq Size: 4047 bp
• RefSeq ORF: 1992 bp
• Locus ID: 11518
• UniProt ID: Q9QYC0
• Cytogenetics: 5 17.9 cM
• Gene Summary: Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the end of an exon and has an alternate exon compared to variant 1 that contains an in-frame stop codon. The resulting isoform (3) contains an internal 31 aa segment not found in isoform 1, but it is still shorter and has a distinct C-terminus compared to isoform 1.