Fzd10 (NM_175284) Mouse Untagged Clone

CAT#: MC219323

Fzd10 (untagged) - Mouse frizzled homolog 10 (Drosophila) (Fzd10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fzd10
• Synonyms: Fz-10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219323 representing NM_175284
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACACCCGGGCCCGCGCCTGTGGCTGGTGCTGCAGGTGATGATAGGCTCGTGCACGGCCATCAGCT
  CCATGGACTTAGAGCGCCCTGGAGACGGCAAGTGCCAGCCGGTGGAGATTCCCATGTGCAAGGACATCGG
  CTACAACACCACCCGCATGCCCAACCTGATGGGTCACGAGAACCAGCGCGAGGCGGCCATCCAACTGCAC
  GAGTTCGCGCCGCTCGTGGAGTACGGCTGCCACAGCCACCTTCGCTTCTTCCTGTGTTCGCTGTACGCGC
  CCATGTGCACCGAGCAGGTCTCCACACCCATCCCTGCTTGCCGGGTCATGTGCGAGCAGGCCCGGCTCAA
  GTGCTCGCCGATCATGGAGCAGTTCAAATTCAGGTGGCCGGACTCCCTGGATTGCAGCAAGCTCCCCAAC
  AAGAACGACCCCAACTACCTGTGCATGGAGGCACCCAACAACGGCTCGGATGAGCCCAGCCGGGGCTCTG
  GCATGTTTCCTCCGCTCTTCAGGCCCCAGAGGCCCCACAGCGCGCAGGAGCACCCACTAAAGGACGGGGG
  TCCGGGGCGCGCAGGTTGTGACAACCCAGGCAAGTTCCACCATGTGGAGAAGAGCGAATCTTGCGCACCG
  CTTTGCACTCCGGGGGTGGATGTGTATTGGAGCCGCGACGACAAGCGCTTCGCTGTGGTCTGGCTGGCCA
  TCTGGTCCGTGCTGTGCTTCTTCTCCAGCGCCTTCACCGTGCTCACCTTCCTCATCGACCCATCGCGCTT
  CAGGTACCCCGAACGTCCTATCATCTTCCTCTCCATGTGCTACTGCGTTTATTCGGTGGGCTATATCATC
  CGCCTCTTCGCGGGCGCGGAGAGCATCGCTTGTGACCGGGACAGTGGGCAGCTGTATGTTATCCAGGAAG
  GACTGGAGAGCACGGGCTGTACCTTAGTCTTCTTGGTACTTTACTACTTCGGCATGGCCAGCTCTTTATG
  GTGGGTGGTCCTCACCCTCACTTGGTTCCTGGCTGCTGGAAAGAAGTGGGGCCATGAGGCCATTGAAGCC
  AACAGCAGCTACTTTCACCTGGCAGCTTGGGCCATCCCGGCTGTGAAGACTATCTTGATCTTGGTGATGC
  GCAGGGTGGCAGGGGATGAGCTCACTGGTGTGTGTTATGTGGGCAGCATGGATGTCAATGCTCTGACCGG
  CTTCGTGCTGGTCCCGCTGGCTTGCTACCTAGTCATCGGCACTTCCTTCATCCTGTCCGGCTTTGTGGCT
  TTATTCCACATCCGGAGGGTGATGAAAACGGGTGGGGAGAACACGGACAAGCTGGAGAAGCTCATGGTAC
  GCATAGGGGTCTTCTCCCTCCTCTACACTGTGCCGGCCACCTGTGTGATTGCCTGCTATTTTTATGAACG
  CCTCAACATGGACTACTGGAAGATGCTGGCCACCCAGCACAAGTGTAAGATGAACAATCAGACCAAGACA
  CCTGACTGCCTGATGACCACCTCCATCCCTGCCGTGGAGGTCTTCATGGTCAAAGTGTCCATGCTGCTGG
  TGGTGGGCATCACCAGTGGGGTGTGGGTCTGGACTTCCAAGACCCTGCAGTCCTGGCAACACGTATGCAG
  CCGGGGGCTAAAGAGAAAAAGCCGGAGGAAACCAGCCAGTGTGGTCACCAGTGCAGGGATCTACAAAAAA
  GCCCAGCACCCCCAAAAACCTCACCTTGGGAAGTATGAACTGCCCGCCCAGCCTTCAGCCTGCGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175284
• Insert Size: 1749 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175284.3, NP_780493.1
• RefSeq Size: 3005 bp
• RefSeq ORF: 1749 bp
• Locus ID: 93897
• UniProt ID: Q8BKG4
• Cytogenetics: 5 G1.3
• Gene Summary: Receptor for Wnt proteins (PubMed:15923619). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:15923619). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).[UniProtKB/Swiss-Prot Function]