Zkscan3 (NM_023685) Mouse Untagged Clone

CAT#: MC218844

Zkscan3 (untagged) - Mouse zinc finger with KRAB and SCAN domains 3 (Zkscan3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zkscan3
• Synonyms: 2810435N07Rik; AA408594; AI132359; mszf35; Skz1; Zf47; Zfp-47; Zfp306; Zfp307
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218844 representing NM_023685
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGAGAATCAAGGGAAAGCACAACCTTGGACTCACACTCTGCAGAGGACCAGATGGAGCTACTGG
  TCATAAAGGTGGAACAAGAAGAGGCCTCCCCCTTGGCAGAGGAGACCAGTTGGCTGGGCAGTCCTGGGCC
  TGACCGCTCCCGCCAGCGCTTCCGTGCTTTCCGCTACCCAGAGGCAGCCGGGCCCCGGCAGGCGCTGAGC
  CGGCTCCGCGAGCTCTGCAGACAGTGGCTGCGGCCAGACATGCACAGCAAGGAGCAGATCCTGGAGCTGC
  TGGTGCTGGAGCAGTTCCTGACCATCCTGCCAGGGGAGCTGCAGGCCTGGGTGCGCGAGCAGCACCCCGA
  CAGCGGGGAGGAGGTGGTGGCGCTGCTGGAGTACTTGGACAGGCAGCTGGATGACACACCTCCACAGGTT
  CCAGATGATGACGATGGGCAGGAACTCCTTTGCTCCAAGGCAGTGCTGTTGACATCAGCTCAGGGCTCAG
  AAAGTAGCCAGATGGAGCCCGTGGAGCCTCTGCTGAAGCAGGAGTCTTTGGGATCCCTGCCCTCAGAAGT
  CAGAGTCACTCATGTGGGGCACTGTGGAGAAGATGGAGTGACAGCTACTAGGCTCACATCGGAATTACAG
  GGGTTGCTGAAAATGGAAGATGTAGCCCCAGTCCTTTCCCCCAGATGGACAGAGCAGGATTCATCTCAGA
  TGAACCTCTACAAAGATGGAATGCAGGAGCACTCTGGCAGCCTGGTTTCCCTGGATCAGGACATGCAGAC
  TAAGGTTAGGGACTTGCCTCGAGCTGAAGAATATAGGGACCAAAAGCCTGAGCAGACAGTGTGCTTCCTG
  GGTGAAGACACTGTCCCGATTCCTACAGGTGCAGAAGCCAGTGAGCAGGAAGGCAAGTTACAGGCAGCAC
  AGAAGAGTGCCACAGGAACCAGGCGGTTCTATTGCCGTGAATGTGGGAAGAGCTTTGCTCAGAGTTCAGG
  CCTAAGTAAACACAAAAGAATCCACACTGGATTGAAACCCTATGAATGTGAGGAGTGTGGCAAAGCCTTC
  ATTGGAAGCTCAGCTCTCATTATTCATCAGAGAGTTCACACTGGTGAGAAGCCATATGAGTGTGAAGAAT
  GTGGTAAGGCCTTCAGTCACAGCTCAGATCTCATCAAACACCAGAGAACCCACACTGGGGAAAAGCCCTA
  CGAGTGTGATGACTGTGGGAAAACCTTCACTCAGAGCTGCAGCCTCCTTGAACATCACAGAATTCACACT
  GGGGAGAAGCCATACCAGTGCAACATGTGTCCCAAAGCCTTTAGGCGTAGCTCACATCTTCTGAGACATC
  AGAGGACCCATACTGGGGATAAAGATTTTTTTGTTCCAGAACCTTACTGGGAAAGTCAGAGTAGGGTGGA
  AAGCCATTGGGAAAATATTGAAACTCCTGTGTCTTATCAATGTAATGATTGTGAGAGAAGTTTCAGTAGG
  ATTACAAGCCTTATTGAACACCAAAAAGTACACACTGGTGAGAAGCCCTTTGAGTGCCAAACCTGTGGAA
  AAGGCTTCACCCGACCTTCATACCTTATTCAACATCAGAGAAGACACACGGGGAAGAAAACTTCTGTCAC
  AGTGACCCCTGCTGTACATTCCGAAGTTGGTGTTCAACTGTCATTGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023685
• Insert Size: 1662 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023685.4, NP_076174.3
• RefSeq Size: 2600 bp
• RefSeq ORF: 1662 bp
• Locus ID: 72739
• UniProt ID: Q91VW9
• Cytogenetics: 13 A3.1
• Gene Summary: Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 both encode the same isoform (a).