Tie1 (BC060182) Mouse Untagged Clone

CAT#: MC218258

Tie1 (untagged) - Mouse tyrosine kinase receptor 1 (cDNA clone MGC:63223 IMAGE:6831929), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tie1
• Synonyms: D430008P04Rik; TIE; tie-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC060182
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGGTGGGGATCCTCTTTGCTGCTCCCCACTCTTTTCTTGGCCTCTCATGTTGGTGCGTCTGTTG
  ACTTAACATTGCTGGCCAACCTGCGCATCACAGACCCCCAGCGTTTCTTCCTGACCTGCGTGTCTGGTGA
  GGCCGGAGCAGGGAGGAGCTCGGACCCGCCCCTGCTGCTGGAGAAGGATGATCGCATCGTGCGCACCTTC
  CCGCCTGGGCAGCCCCTGTACCTGGCACGCAACGGTTCTCACCAGGTCACGCTGAGGGGCTTCTCCAAGC
  CCTCGGATTTGGTAGGCGTCTTCTCCTGTGTGGGTGGTGCTGGCGCGCGGCGCACTCGAGTTCTCTATGT
  GCACAACAGCCCAGGGGCACACCTGTTTCCAGACAAGGTCACACACACGGTGAACAAAGGTGACACTGCT
  GTGCTTTCTGCCCATGTGCACAAGGAAAAGCAGACTGACGTGATCTGGAAGAACAACGGATCCTACTTCA
  ATACCCTAGACTGGCAAGAGGCCGATGACGGGCGTTTTCAACTGCAGCTCCAAAATGTACAGCCACCATC
  AAGTGGCATCTACAGTGCCACCTACCTAGAGGCCAGCCCCCTGGGCAGTGCCTTCTTTCGGCTCATCGTG
  CGAGGCTGTGGGGCTGGACGCTGGGGGCCAGGATGTGTCAAGGATTGCCCAGGCTGCCTGCATGGAGGTG
  TCTGTCATGACCATGATGGCGAATGTGTGTGTCCCCCAGGATTCACTGGCACCCGCTGTGAGCAGGCCTG
  CAGGGAGGGTCGTTTTGGACAGAGCTGTCAGGAACAGTGCCCAGGCACAGCAGGTTGTAGAGGTCTCACC
  TTCTGCCTCCCGGATCCCTATGGCTGTTCCTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAGGAAGCAT
  GTGCACCTGGTCATTTTGGGGCTGATTGTCGCCTCCAGTGCCAGTGTCAAAATGGTGGCACTTGTGACCG
  GTTCAGCGGCTGCGTCTGCCCCTCTGGGTGGCATGGAGTCCACTGTGAAAAGTCAGACCGGATCCCCCAG
  ATCCTCAGTATGGCCACAGAGGTGGAGTTCAACATAGGGACGATGCCCCGGATCAACTGTGCAGCTGCCG
  GGAATCCCTTCCCAGTGCGGGGCAGCATGAAACTTCGCAAGCCAGATGGCACCATGCTTTTGTCTACCAA
  AGTCATTGTGGAGCCAGACAGGACCACAGCAGAGTTTGAAGTGCCCAGTTTGACTCTTGGGGACAGTGGG
  TTCTGGGAATGCCGCGTATCGACTTCTGGTGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTCAAAGTAC
  CCCCAGTGCCTTTGACTGCACCTCGACTCCTGGCCAAGCAGAGCCGTCAGCTTGTGGTCTCCCCACTGGT
  CTCCTTTAGTGGGGATGGACCCATCTCCTCTGTCCGCCTGCACTACCGGCCCCAGGACAGCACGATTGCC
  TGGTCTGCCATTGTGGTGGATCCCAGTGAGAATGTGACATTAATGAACCTGAAGCCAAAGACAGGATACA
  ATGTCCGAGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAAGGAGGCTGGGGACCTTCTGCCCTTATGAC
  TACTGACTGTCCTGAGCCTTTGCTGCAGCCCTGGCTAGAGAGCTGGCACGTGGAGGGTCCTGACAGGCTC
  CGTGTGAGCTGGTCTCTACCCTCGGTGCCACTGTCCGGTGATGGTTTCCTGCTGCGCCTGTGGGATGGGG
  CCCGGGGACAGGAGAGGCGGGAGAACATCTCATTCCCCCAGGCCCGCACTGCCCTCCTGACTGGACTCAC
  GCCGGGCACCCACTACCAGCTGGATGTGCGACTGTATCACTGTACCCTCCTGGGCCCTGCCTCACCCCCT
  GCGCATGTGCACCTGCCCCCCAGCGGTAGGCCCACTCTGGGAAGGATGGGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC060182
• Insert Size: 1944 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060182, AAH60182
• RefSeq Size: 4259 bp
• RefSeq ORF: 1943 bp
• Locus ID: 21846
• Cytogenetics: 4 54.67 cM
• Gene Summary: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.[UniProtKB/Swiss-Prot Function]