Pnkd (BC058945) Mouse Untagged Clone

CAT#: MC217802

Pnkd (untagged) - Mouse paroxysmal nonkinesiogenic dyskinesia (cDNA clone MGC:66659 IMAGE:6812127), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pnkd
• Synonyms: Tahccp2, MR-1, MNCb-5687
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058945
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGTGGTAGCTGCTACGGCGCTGAAGGGCCGGGGGGCGAGAAATGCCCGCGTCCTCCGGGGGA
  TCCTCTCTGGAGCCACAGCTAACAAGGCTTCCCAGAACAGGACCAGAGCACTGCAGAGCCACAGCTCACC
  AGAATGCAAGGAGGAGCCCGAGCCCCTCTCCCCTGAGCTAGAATACATTCCCAGAAAGAGGGGCAAGAAC
  CCCATGAAAGCCGTGGGGCTAGCCTGGGCCATCGGCTTCCCCTGTGGTATCCTCTTCTTCGTCCTCACCA
  AGCAGGAAGTGGACAAGGACCGATTGAAGCAGATGAAAGCTCGTCAGAACATGCGGGTGTCCAACACGGG
  CGAGTATAGCCTGTACACCCGAACCTGGCTTGGGTACCTCTTCTACCGACAGCAGCTTCGGAGGGCTCGG
  AATCGCTATCCCAAAGGCCACTCCAAAACTCAGCCCCGCCTCTTCAATGGAGTGAAGGTGCTTCCCATCC
  CTGTCCTCTCGGACAACTACAGCTACCTCATCATCGACACCCAGGCTGGGCTGGCAGTGGCTGTGGACCC
  CTCAGACCCGAGGGCTGTGCAGGCTTCCATTGAAAAGGAAAGAGTTAACTTGGTTGCCATTCTCTGCACC
  CACAAGCACTGGGACCACAGTGGAGGGAACCGTGACCTCAGCCGGCGGCACAGGGACTGTCGAGTGTATG
  GGAGCCCTCAGGATGGTATCCCCTACCTCACCCACCCCCTGTGTCATCAAGATGTGGTCAGCGTGGGACG
  ACTTCAGATTCGAGCCCTAGCCACCCCTGGCCACACTCAAGGCCATCTGGTCTACCTGCTGGATGGGGAG
  CCCTACAAAGGTCCTTCCTGCCTCTTCTCAGGGGACCTGCTCTTCCTCTCTGGCTGTGGACGGACCTTCG
  AAGGCACAGCGGAGACCATGCTGAGCTCACTGGACACTGTGTTAGACCTGGGGGATGACACCCTGCTGTG
  GCCTGGGCATGAATACGCAGAAGAGAACCTGGGCTTTGCAGGCGTGGTGGAGCCCGAGAACTTGGCTCGT
  GAGAGGAAGATGCAGTGGGTACAGAGGCAACGGATGGAACGCAAGAGCACATGCCCATCCACCCTGGGAG
  AGGAACGCGCCTACAACCCATTCCTGAGAACCCATTGCCTGGAACTACAGGAGGCTCTGGGGCCAGGGCC
  AGGCCCCACCAGTGATGACGGCTGCTCCCGGGCCCAGCTCCTGGAGGAGCTGCGCCGCCTGAAGGACATG
  CATAAAAGCAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC058945
• Insert Size: 1275 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058945, AAH58945
• RefSeq Size: 3199 bp
• RefSeq ORF: 1274 bp
• Locus ID: 56695
• Cytogenetics: 1 C3
• Gene Summary: Probable hydrolase that plays an aggravative role in the development of cardiac hypertrophy via activation of the NF-kappa-B signaling pathway.[UniProtKB/Swiss-Prot Function]