Gpt2 (NM_173866) Mouse Untagged Clone

CAT#: MC217501

Gpt2 (untagged) - Mouse glutamic pyruvate transaminase (alanine aminotransferase) 2 (Gpt2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpt2
• Synonyms: 4631422C05Rik; ALT2; AU014768; AU041193; C87201
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217501 representing NM_173866
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGGCAGCGGTGCTGGTGCGGCGGGGCTCCTGCCCCCGCGCCTCGGGCCCCTGGGGCCGTAGTC
  ACAGCAGCGCTGCAGCCGAAGCCTCGGCGGCGCTCAAGGTGCGACCGGAGCGCAGCCCTCGAGACCGCAT
  CCTCACCCTGGAGTCCATGAACCCGCAGGTGAAGGCGGTGGAGTACGCTGTGCGGGGACCCATCGTGCTC
  AAAGCCGGCGAGATCGAGATGGAGCTGCAGCGGGGTATCAAAAAACCATTCACTGAGGTAATCCGAGCCA
  ACATTGGGGATGCCCATGCTATGGGCCAGCAGCCAATCACCTTCCTCCGTCAGGTGATGGCACTCTGCAC
  CTACCCAAACCTACTAAACAGCCCCAGCTTCCCAGAAGACGCTAAGAAACGAGCGCGGCGGATCCTGCAG
  GCTTGTGGTGGAAACAGCTTGGGATCTTACAGTGCTAGCCAAGGCGTTAACTGTATCCGTGAAGATGTGG
  CAGCCTTTATCACCAGGAGAGATGGTGTGCCTGCAGACCCAGACAACATTTACCTGACTACTGGAGCTAG
  CGACGGTATTTCTACAATCCTGAAGCTCCTGGTCTCCGGTGGTGGCAAGTCACGGACCGGCGTGATGATT
  CCCATCCCCCAGTATCCCTTGTACTCCGCGGTCATCTCTGAGCTCGACGCGGTGCAGGTCAACTACTATC
  TGGATGAAGAGAACTGCTGGGCTTTGAATGTGGACGAGCTCCGGCGGGCATTGCGGCAAGCCAAAGACCA
  CTGTGACCCTAAAGTTCTCTGCATTATCAACCCCGGAAACCCCACAGGCCAGGTACAAAGCAGAAAGTGC
  ATAGAAGATGTGATTCACTTTGCCTGGGAAGAGAAGCTTTTTCTCCTGGCTGATGAGGTGTACCAGGACA
  ACGTGTACTCTCCAGACTGCAGATTCCACTCGTTTAAGAAAGTGCTTTACCAGATGGGGCACGAGTACTC
  CAGCAACGTGGAGCTCGCCTCCTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGTGGCTACAGAGGG
  GGCTACATGGAGGTGATCAATTTGCACCCCGAGATCAAAGGCCAGCTGGTGAAGCTACTCTCGGTTCGCC
  TCTGTCCGCCAGTGTCAGGACAGGCCGCCATGGACATTGTTGTGAATCCACCGGAACCAGGAGAGGAGTC
  CTTTGAGCAATTCAGCAGGGAAAAAGAATTCGTCCTTGGTAATCTGGCCAAAAAAGCAAAGCTGACAGAA
  GATCTGTTTAACCAAGTCCCAGGGATCCAGTGCAACCCCTTGCAAGGAGCTATGTATGCGTTCCCTCGGA
  TTCTCATCCCTGCCAAGGCCGTGGAGGCAGCTCAGTCCCATAAAATGGCTCCAGACATGTTCTACTGCAT
  GAAGCTCCTGGAGGAGACTGGCATCTGTGTCGTGCCTGGCAGTGGCTTTGGGCAGCGAGAAGGCACTTAC
  CACTTCAGAATGACCATTCTCCCTCCGGTGGATAAACTGAAGACCGTGCTCCACAAGGTGAAAGACTTTC
  ACCTGAAGTTCCTGGAGCAGTACTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173866
• Insert Size: 1569 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173866.3, NP_776291.1
• RefSeq Size: 3596 bp
• RefSeq ORF: 1569 bp
• Locus ID: 108682
• UniProt ID: Q8BGT5
• Cytogenetics: 8 C3
• Gene Summary: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.[UniProtKB/Swiss-Prot Function]