Slc44a1 (NM_001159633) Mouse Untagged Clone

CAT#: MC217490

Slc44a1 (untagged) - Mouse solute carrier family 44, member 1 (Slc44a1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc44a1
• Synonyms: 2210409B22Rik; 4833416H08Rik; AW547365; Cdw92; CHTL1; CTL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217490 representing NM_001159633
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCTGCAGCTCCGCCTCCGCCGCGCAGAGCTCCAAACGAGAATGGAAGCCATTGGAAGACCGTA
  GCTGCACAGACATACCATGGCTGCTTCTCTTTGTTCTCTTCTGCATTGGGATGGGATTTATTTGTGGCTT
  TTCAGTAGCAACAGGTGCAGCAGCAAGACTAGTGTCAGGATACGACAGCTATGGAAATATCTGTGGGCAG
  AGAAATGCAAAACTGGAAGCAATACCGAACAGTGGTCTGGACCACACCCACCGGAAGTATGTGTTCTTTT
  TGGACCCATGCAACCTGGACTTGATAAACAGGAAGATCAAGTCTATAGCACTGTGCGTAGCAGCATGTCC
  TCGACAGGAGTTGAAAACCTTGAGTGACGTCCAGAAGTTTGCAGAGATCAATGGCTCAGCACTATGTAGC
  TACAACATAAAGCCTTCTGAATATACTCTGACTTCAAAATCCTCTGGCTTTTGCCCCAAGCTACCAGTAC
  CAGCCAGTGCACCTATTCCATTCTTCCATCGCTGTGCTCCTGTGAACATTTCCTGCTATGCCAAGTTTGC
  AGAGGCCCTGATCACCTTTGTCAGTGACAATAGTGTCTTACACAGGCTGATTAGTGGAGTAATGACCAGC
  AAAGAAATTATATTGGGACTTTGCTTGTTATCACTAGTTCTGTCCATGATCCTGATGGTGATTATCAGGT
  ATATATCAAGAGTGCTAGTGTGGATTCTAACAGTTCTGGTCATACTGGGTTCACTTGGTGGCACGGGTGT
  ATTATGGTGGCTGTATGCAAAGCAAAGAAGGTCCCCCAAAGAAGCAGTTATTCCTGAGCAGCTTCAGATA
  GCTGAGGACAATCTGCGGGCGCTCCTCATCTATGCCATTTCAGCGACAGTGTTCACTGTCATTCTCTTCC
  TGATCATGCTGGTTATGCGCAAGCGTGTGGCTCTCACCATCGCCTTGTTCCACGTGGCTGGCAAGGTCTT
  CATACACTTGCCTCTGTTAGTCTTCCAGCCCTTCTGGACGTTTTTTGCTCTCGTCTTATTTTGGGCATAC
  TGGATAATGACACTTCTCTTCCTTGGCACTACTGGCAGTGCTGTTCAGAATGAGCAAGGCTTCGTGGAGT
  ACAAAATCTCCGGGCCTTTGCAGTACATGTGGTGGTACCACGTGGTTGGCCTGATTTGGATCAGTGAGTT
  CATTCTCGCCTGTCAGCAGATGACTGTGGCAGGAGCTGTGGTAACATACTACTTTACTAGGGATAAACGG
  AATTTGCCTTTCACACCTATTCTGGCATCAGTGAATCGCCTTATTCGTTACCACCTCGGTACTGTGGCAA
  AAGGATCCTTCATTATCACCCTCGTCAAAATCCCCAGGATGGTCCTCATGTATATCCACAGTCAGCTCAA
  AGGGAAGGAAAACGCTTGTGCACGATGCATGCTGAAATCCTGCATTTGCTGCCTTTGGTGTCTTGAAAAG
  TGCCTGAGCTACTTAAACCAGGTTTGCATGCAGCCTTTGAAAGCCTGGAGCAGAGCCAGAAAACTGAAGC
  ATGCAAGGGGCAGTTGGGGCATCTCAGACAAGAGACAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159633
• Insert Size: 1581 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001159633.1, NP_001153105.1
• RefSeq Size: 1938 bp
• RefSeq ORF: 1581 bp
• Locus ID: 100434
• Cytogenetics: 4 B2
• Gene Summary: Choline transporter. May be involved in membrane synthesis and myelin production.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has a different exon for its 3'-terminal exon, compared to variant 1, which results in a distinct 3' UTR and a protein (isoform B) with a shorter and distinct C-terminus, compared to isoform A.