Wrap73 (NM_021499) Mouse Untagged Clone

CAT#: MC216310

Wdr8 (untagged) - Mouse WD repeat domain 8 (Wdr8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wrap73
• Synonyms: 2610044M17Rik; 5330425N03Rik; Dd57; Wdr8; Wrap73
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216310 representing NM_021499
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTCTCGGAGTCATTCAAGCTCTCGGGCCTGCTCTGCAGGTTCTCCCCGGACGGCAAGTACCTGG
  CTTCCTGTGTCCAGTACCGATTAGTGATCCGGGATGTGACGACCCTTCAGATCCTTCAGTTGTACACATG
  CCTGGACCAGATCCAGCACATAGAGTGGTCTGCAGACTCCCTCTTCATCCTGTGTGCCATGTACAGGCGG
  GGGCTTGTGCAGGTCTGGTCACTCGAGCAGCCAGAATGGCACTGCAAGATCGACGAGGGCTCTGCGGGGC
  TGGTCGCTTCCTGCTGGAGTCCAGATGGCCGCCACATCCTGAACACAACCGAATTTCATCTGCGAATCAC
  GGTCTGGTCCCTGTGCACCAAATCTGTATCTTACATCAAGTATCCCAAGGCCTGCCAGCAGGGACTAACT
  TTCACCAGGGATGGCCGCTACCTGGCCTTAGCAGAGAGGCGGGACTGCAGGGACTATGTCAGCATCTTTG
  TGTGCAGTGACTGGCAGCTCCTCCGGCACTTTGACACAGACACCCAGGATCTCACAGGGATCGAGTGGGC
  CCCGAATGGCTGTGTACTGGCCGCGTGGGACACCTGCTTGGAGTACAAGGTTCTGCTGTACTCCTTGGAC
  GGCCGCCTGCTGTCAGCATACTGTGCCTATGAATGGTCCCTGGGCATCAAGTCTGTGGCTTGGAGCCCCA
  GCAGTCAGTTCCTGGCCATTGGGAGCTACGATGGAAAGGTGCGCCTCCTAAATCACGTGACCTGGAAGAT
  GATAACTGAGTTTGGGCATCCTGCAACCATTAACAACCCCAAGACAGTTGTGTATAAGGAAGCTGAGAAG
  AGCCCACTGCTGGGGTTGGGCCACCTGTCCTTCCCGCCGCCCCGAGCAATGGCTGGTGCCCTCTCTACCT
  CAGAGAGCAAATATGAGATCGCCTCGGGACCAGTTTCCTTGCAGACTCTGAAGCCAGTAGCTGACAGAGC
  GAACCCGAGGATGGGTGTAGGGATGCTGGCTTTCAGCTCTGACAGCTACTTCCTGGCATCAAGAAATGAC
  AATGTCCCCAATGCTGTCTGGATCTGGGACATTCAAAAGCTGAAGCTGTTTGTGGTACTAGAGCATATGT
  CTCCAGTGCGCTCATTTCAGTGGGACCCACAGCAGCCGAGGCTGGCCATCTGCACAGGAGGAAGCAAGGT
  GTACCTGTGGTCCCCAGCAGGCTGCGTGTCGGTGCAGGTGCCTGGGGAAGGTGACTTTCCAGTGCTCGGA
  CTGTGCTGGCACTTAAGTGGGGATTCCTTGGCCCTCCTTAGTAAGGACCACTTCTGTCTCTGCTTCCTGG
  AGACCAAGGAGAGGGTTGGCACAGCCTACGAACAGCGGGACGGCATGCCTAGGACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021499
• Insert Size: 1389 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021499.2, NP_067474.2
• RefSeq Size: 1600 bp
• RefSeq ORF: 1389 bp
• Locus ID: 59002
• UniProt ID: Q9JM98
• Cytogenetics: 4 E2
• Gene Summary: The SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Required for the centrosomal localization of SSX2IP and normal mitotic bipolar spindle morphology. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP, CEP290 and PIBF1/CEP90. Required for ciliogenesis and involved in the removal of the CEP97:CCP110 complex from the mother centriole. Involved in ciliary vesicle formation at the mother centriole and required for the docking of vesicles to the basal body during ciliogenesis; may promote docking of RAB8A- and ARL13B-containing vesicles (By similarity).[UniProtKB/Swiss-Prot Function]