Pla2g7 (NM_013737) Mouse Untagged Clone

CAT#: MC215815

Pla2g7 (untagged) - Mouse phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) (Pla2g7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pla2g7
• Synonyms: PAF-AH; R75400
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215815 representing NM_013737
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTACCACTCAAACTGCAGGCGCTTTTCTGCCTCCTCTGCTGCCTCCCATGGGTCCATCCTTTTCACT
  GGCAAGACACATCTTCTTTTGACTTCAGGCCGTCAGTAATGTTTCACAAGCTCCAATCGGTGATGTCTGC
  TGCCGGCTCTGGCCATAGTAAAATCCCCAAAGGAAATGGATCGTACCCCGTCGGTTGTACAGATCTGATG
  TTCGGTTATGGGAATGAGAGCGTCTTCGTGCGTTTGTACTACCCAGCTCAAGATCAAGGTCGCCTCGACA
  CTGTTTGGATCCCAAACAAAGAATATTTTTTGGGTCTTAGTATATTTCTTGGAACACCCAGTATTGTAGG
  CAATATTTTACACCTCTTATATGGTTCTCTGACAACTCCTGCAAGCTGGAATTCTCCTTTGAGGACTGGA
  GAAAAATACCCACTCATTGTCTTTTCTCATGGTCTCGGAGCCTTCAGGACGATTTATTCTGCTATTGGCA
  TTGGCTTGGCATCTAATGGGTTTATAGTGGCCACTGTCGAACACAGAGACAGATCTGCATCGGCAACTTA
  CTTTTTTGAAGACCAGGTGGCTGCAAAAGTGGAAAACAGGTCTTGGCTTTACCTGAGAAAAGTAAAACAA
  GAGGAGTCGGAAAGTGTCCGGAAAGAACAGGTTCAGCAAAGAGCAATAGAATGTTCCCGGGCTCTCAGTG
  CGATTCTTGACATTGAACATGGAGACCCAAAAGAGAATGTACTAGGTTCAGCTTTTGACATGAAACAGCT
  GAAGGATGCTATTGATGAGACTAAAATAGCTTTGATGGGACATTCTTTTGGAGGAGCAACAGTTCTTCAA
  GCCCTTAGTGAGGACCAGAGATTCAGATGTGGAGTTGCTCTTGATCCATGGATGTATCCGGTGAACGAAG
  AGCTGTACTCCAGAACCCTCCAGCCTCTCCTCTTTATCAACTCTGCCAAATTCCAGACTCCAAAGGACAT
  CGCAAAAATGAAAAAGTTCTACCAGCCTGACAAGGAAAGGAAAATGATTACAATCAAGGGCTCAGTGCAC
  CAGAACTTTGACGACTTTACTTTTGTAACTGGCAAAATAATTGGAAACAAGCTGACACTGAAAGGAGAAA
  TCGATTCCAGAGTAGCCATCGACCTCACCAACAAAGCTTCGATGGCTTTCTTACAAAAGCATTTAGGGCT
  TCAGAAAGACTTTGATCAGTGGGACCCTCTGGTGGAAGGAGATGATGAGAACCTGATTCCTGGGTCACCC
  TTTGACGCAGTCACCCAGGTCCCGGCTCAGCAACACTCTCCAGGATCACAGACCCAGAATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013737
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013737.5, NP_038765.2
• RefSeq Size: 1923 bp
• RefSeq ORF: 1323 bp
• Locus ID: 27226
• UniProt ID: Q60963
• Cytogenetics: 17 B3
• Gene Summary: Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.[UniProtKB/Swiss-Prot Function]