Fbxo32 (NM_026346) Mouse Untagged Clone

CAT#: MC210615

Fbxo32 (untagged) - Mouse F-box protein 32 (Fbxo32), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fbxo32
• Synonyms: 4833442G10Rik; AI430017; ATROGIN1; Gm20361; MAFbx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210615 representing NM_026346
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTTCCTTGGGCAGGACTGGCGGTCCCCGGGCCAGAGCTGGGTGAAGACGGCGGACGGCTGGAAGC
  GCTTCTTGGATGAGAAAAGCGGCAGCTTCGTGAGCGACCTCAGCAGTTACTGCAACAAGGAGGTATACAG
  TAAGGAGAATCTGTTCAGCAGCCTGAACTACGACGTCGCAGCCAAGAAGAGAAAGAAAGACATTCAGAAC
  AGCAAAACCAAAACTCAGTACTTCCATCAAGAAAAGTGGATCTATGTTCACAAAGGAAGTACGAAGGAGC
  GCCATGGATACTGTACTTTGGGGGAAGCTTTCAACAGACTGGACTTCTCGACTGCCATCCTGGATTCCAG
  AAGATTCAACTACGTAGTAAGGCTGTTGGAGCTGATAGCAAAGTCACAGCTCACATCCCTGAGTGGCATC
  GCCCAAAAGAACTTCATGAACATTTTGGAAAAAGTGGTACTGAAAGTTCTTGAAGACCAGCAAAACATAA
  GACTTATACGGGAACTTCTCCAGACTCTCTACACATCCTTATGCACACTGGTGCAGAGAGTCGGCAAGTC
  TGTGCTGGTGGGCAACATTAACATGTGGGTGTATCGGATGGAGACCATTCTACACTGGCAGCAGCAGCTG
  AATAGCATCCAGATCAGCAGGCCTGCCTTCAAAGGCCTCACGATCACCGACCTGCCTGTGTGCTTACAAC
  TGAACATCATGCAGAGGCTGAGTGACGGGCGGGACCTGGTCAGCCTGGGCCAGGCAGCCCCAGACCTGCA
  TGTGCTCAGTGAGGACCGGCTACTGTGGAAGAGACTCTGCCAGTACCACTTCTCAGAGAGGCAGATTCGC
  AAGCGTTTGATCTTGTCTGACAAAGGGCAGCTGGATTGGAAGAAGATGTATTTTAAGCTTGTACGATGTT
  ACCCAAGAAGAGAGCAGTATGGGGTCACCCTGCAGCTTTGCAAACACTGCCACATTCTCTCCTGGAAGGG
  CACTGACCATCCGTGCACGGCCAACAACCCAGAGAGCTGCTCCGTCTCACTTTCCCCTCAAGACTTTATC
  AATTTGTTCAAGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026346
• Insert Size: 1068 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_026346.3, NP_080622.1
• RefSeq Size: 6936 bp
• RefSeq ORF: 1068 bp
• Locus ID: 67731
• UniProt ID: Q9CPU7
• Cytogenetics: 15 D1
• Gene Summary: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1 (By similarity).[UniProtKB/Swiss-Prot Function]