Bccip (NM_025392) Mouse Untagged Clone

CAT#: MC210288

Bccip (untagged) - Mouse BRCA2 and CDKN1A interacting protein (Bccip), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bccip
• Synonyms: 1110013J05Rik; TOK-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210288 representing NM_025392
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTAAGGCTAAGAAGCGAGCGGTGGGAAATGGAATTCAGCGGCCGCTGGGAGCCCCGGGCCAGC
  GGGAGGAGGAAGAAGAAGAAGAGGATGAGGTGGAGGATGAGGAAGAAGACGAGGACGACAGTGACGAAGA
  AGAGGATGAAGTTGACGAAATCGTGGACGAGGAAGTGAATATTGAGTTTGAAGCCTATTCCATCTCAGAT
  AACGATTATGGCGGAATTAAGAAATTACTGCAACAGCTTTTTCTAAAAGCCCCCGTGAACACTGCCGAGC
  TGACGAATCTCCTGATGCAGCAGAACCACATTGGGAGTGTGATTAAGCAAACAGATGTTTCAGAAGACAG
  TGATGATGAAGTGGATGAAGATGAGATCTTTGGCTTCATAAGCCTTTTAAATTTGACTGAAAGAAAGGGG
  ACTCAGTGTGCTGAACAAATTAAAGAGTTGGTTCTAAGCTTCTGTGAGAAGACCTGTGAGCAGAGCATGG
  TTGAACAGCTGGACAAGCTTTTGAATGACACCAGCAAGCCTGTGGGCCTTCTGCTAAGTGAAAGATTCAT
  TAACGTCCCTCCACAGATTGCCCTGCCCATGCACCAGCAGCTCCAGAAAGAACTGTCTGAGGCGCGAAGA
  ACCAACAAGCCATGTGGGAAATGCTGCTTCTACCTGCTGATTAGTAAGACATTTATGGAAGCAGGAAAGT
  CCAGTTCCAGAAAGAGGCAGGACAGCCTTCAGCAGGGGGCACTGATGTTTGCAAATGCAGAGGAAGAGTT
  CTTCTATGAGAAGGCAATCTTGAAGTTCAGCTACTCGGTGCAGGGGGAGAGCGACACTCGTCTGGGAGGC
  AGATGGTCCTTCGATGATGTTCCGATGACGCCTTTGCGGACTGTGATGGTAATTCCAGACGACAGGATGA
  ATGAGATCATGGAAACCCTGAAAGACCATCTCTCCGTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025392
• Insert Size: 951 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025392.2, NP_079668.2
• RefSeq Size: 1260 bp
• RefSeq ORF: 951 bp
• Locus ID: 66165
• UniProt ID: Q9CWI3
• Cytogenetics: 7 F3
• Gene Summary: During interphase, required for microtubule organizing and anchoring activities. During mitosis, required for the organization and stabilization of the spindle pole (PubMed:28394342). May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ) (By similarity).[UniProtKB/Swiss-Prot Function]