Rabgap1l (NM_001038621) Mouse Untagged Clone

CAT#: MC209810

Rabgap1l (untagged) - Mouse RAB GTPase activating protein 1-like (Rabgap1l), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rabgap1l
• Synonyms: 5830411O09Rik; 8430421H08Rik; 9630005B12Rik; AW049894; Hh1; HHL; mKIAA0471
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209810 representing NM_001038621
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAAGGGGTGCCCTGCCCAGCCCCAGCTGCTAAGCTCACACCTCCTGTCAAAAAGTCCCAGGACA
  TGCACGACGAGAGGAGCAAGCTGGTGAATGAGTATGCATGTCGAGTGCTGGAACTTCTGGGGATGGGGCA
  TCGGCTGTTTGTGCCACGGCTTCTGGCGACCTCAAAGGAAGACCTCCTTCAGGCTGATTTTGAAGGTGCT
  TTGAAGTTTTTCAGAGTTCAGCTTCCAAAGAGATACCGGGCAGAGGAGAATGCAAGAAGATTGATGGAAC
  AGGCTTGCAATATTAAAGTACCAACCAAAAAGCTGAAGAAATATGAAAAAGAATATCAGGCGATGCGGGA
  GAATCAGCTGCAGCAGGAGGACCCAATGGATAGATACAAGAGGGAGAACCGAAGATTACAGGAGGCCAGC
  ATGAGGTTGGAACAAGAAAATGATGACCTTGCCCATGAACTAGTGACAAGCAAAATTGCTCTGCGGAATG
  ACTTGGATCAGGCAGAAGACAAGGCTGATGTGCTGAATAAGGAGCTCCTCTTTACCAAGCAGAGGTTGGT
  GGAGACTGAGGAAGAGAAGAGGAAGCAAGAGGAGGAGACCGCCCAGCTAAAAGAAGTCTTCAGGAAACAG
  TTAGAAAAGGCAGAATATGAGATAAAGAAGACAACAGCTATCATTGCTGAGTATAAACAGATATGTTCTC
  AATTAAGTACCAGGCTGGAGAAACAGCAAGCAGCCAGCAAGGAGGAGCTAGAAGCAGTAAAGGGTAAGAT
  GATGGCATGCAAACACTGCAGTGATATCTTCAGCAAGGAAGGAGCTTTAAAGCCAGTGGCCGTAAATAGA
  GAGGACCAGGGACTTGAAGCAGATGATGAAAAGGACTCACTTAAGAAGCAGCTGAGGGAGATGGAACTGG
  AACTGGCACAGACCAAACTGCAGCTGGTGGAAGCCAAGTGCAAAATCCAGGAACTTGAGCATCAGAGAGG
  AGCGCTTATGAATGAAATACAAGCTGCAAAAAACTCTTGGTTTAGTAAAACCCTGAACTCTATCAAAACA
  GCCACAGGCACTCAGCCACTACAGCCGCCACAGGCTCCCCAGCCACCCAAGGAGAGCACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001038621
• Insert Size: 1113 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038621.2, NP_001033710.1
• RefSeq Size: 6338 bp
• RefSeq ORF: 1113 bp
• Locus ID: 29809
• UniProt ID: A6H6A9
• Cytogenetics: 1 H2.1
• Gene Summary: GTP-hydrolysis activating protein (GAP) for small GTPase RAB22A, converting active RAB22A-GTP to the inactive form RAB22A-GDP (By similarity). Plays a role in endocytosis and intracellular protein transport. Recruited by ANK2 to phosphatidylinositol 3-phosphate (PI3P)-positive early endosomes, where it inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (PubMed:27718357).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a.