Idh3g (NM_008323) Mouse Untagged Clone

CAT#: MC208738

Idh3g (untagged) - Mouse isocitrate dehydrogenase 3 (NAD+), gamma (Idh3g), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Idh3g
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208738 representing NM_008323
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGAAGGTGGCGATAGCTGCTGGCGGTGCTGCAAAGGCAATGCTCAAGCCAACTCTCCTCTGCC
  GTCCTTGGGAGGTTCTGGCTGCCCATGTGGCCCCCCGAAGGAGCATTTCCTCACAACAAACAATTCCTCC
  ATCTGCTAAGTATGGTGGGCGGCATACAGTGACTATGATCCCAGGGGATGGCATCGGCCCAGAGCTCATG
  TTGCATGTTAAGTCTGTATTCAGGCATGCATGTGTGCCGGTGGACTTTGAAGAGGTGCATGTAAGCTCCA
  ACGCTGATGAGGAGGACATCCGCAATGCCATCATGGCCATCCGCCGGAACCGTGTGGCCCTGAAGGGCAA
  CATTGAAACAAATCATAACCTGCCACCATCCCACAAATCTCGAAACAACATCCTTCGCACCAGCCTAGAC
  CTCTATGCCAACGTCATCCACTGTAAGAGCCTGCCAGGAGTGGTGACCCGGCACAAGGACATAGACATCC
  TCATTGTACGGGAAAACACAGAAGGCGAGTACAGCAGCCTGGAGCATGAGAGCGTAGCAGGAGTGGTGGA
  GAGCTTGAAGATTATCACCAAAGCCAAGTCCCTGCGCATTGCTGAATATGCTTTCAAGCTGGCCCAGGAG
  AGTGGGCGTAAGAAAGTGACGGCTGTGCACAAGGCCAACATCATGAAACTGGGTGATGGACTCTTCCTCC
  AGTGCTGCAGGGAAGTAGCAGCCCACTACCCTCAGATCACCTTTGACAGCATGATTGTAGACAACACAAC
  AATGCAGCTGGTATCCCGGCCTCAGCAGTTTGATGTCATGGTGATGCCTAATCTCTATGGTAACATTGTC
  AACAACGTCTGTGCAGGGCTAGTTGGAGGCCCAGGCCTTGTGGCTGGGGCCAACTATGGCCATGTGTATG
  CAGTATTCGAGACAGCTACAAGGAACACAGGCAAAAGTATTGCCAATAAGAACATTGCTAACCCGACTGC
  CACACTGCTAGCAAGCTGCATGATGCTAGACCACCTCAAGCTCCACTCCTATGCCACTTCCATCCGCAAA
  GCTGTCTTAGCATCCATGGACAATGAAAATATGCATACCCCAGATATTGGAGGCCAGGGCACCACATCCC
  AAGCCATCCAGGACATCATTCGTCATATCCGCATCATTAATGGACGGGCTGTGGAGGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008323
• Insert Size: 1182 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008323.1, NP_032349.1
• RefSeq Size: 1335 bp
• RefSeq ORF: 1182 bp
• Locus ID: 15929
• UniProt ID: P70404
• Cytogenetics: X 37.41 cM
• Gene Summary: Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.[UniProtKB/Swiss-Prot Function]