Ndufs4 (NM_010887) Mouse Untagged Clone

CAT#: MC207332

Ndufs4 (untagged) - Mouse NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ndufs4
• Synonyms: 6720411N02Rik; C1-18k
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207332 representing NM_010887
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGTCTCAATGTCAGTGTCGCTGAGACAGGCGATGTTGGGGCGAAGGGCAATGGCTACAGCTG
  CCGTTTCCGTCTGTAGAGTTCCATCCAGGTTGTTGAGCACATCCACTTGGAAGCTGGCAGACAACCAGAC
  TCGGGACACACAGCTTATAACAGTTGATGAGAAACTGGATATCACAACTTTAACTGGTGTTCCAGAAGAG
  CACATCAAAACCAGAAAGGTCAGAATCTTTGTTCCTGCTCGCAATAACATGCAGTCTGGAGTAAATAACA
  CAAAGAAATGGAAGATGGAGTTTGATACCAGAGAGAGATGGGAAAATCCTTTGATGGGTTGGGCATCAAC
  CGCTGACCCCCTCTCCAACATGGTTCTGACCTTCAGTGCCAAAGAAGATGCAATTGCCTTTGCAGAAAAA
  AACGGATGGAGCTATGATGTGGAAGAGAAGAAGGTTCCGAAACCCAAGTCCAAGTCTTATGGTGCAAACT
  TTTCTTGGAACAAAAGAACAAGAGTGTCTACAAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010887
• Insert Size: 528 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010887.2, NP_035017.2
• RefSeq Size: 1534 bp
• RefSeq ORF: 528 bp
• Locus ID: 17993
• UniProt ID: Q9CXZ1
• Cytogenetics: 13 D2.2
• Gene Summary: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.[UniProtKB/Swiss-Prot Function]