Mbd2 (NM_010773) Mouse Untagged Clone
CAT#: MC204466
Mbd2 (untagged) - Mouse methyl-CpG binding domain protein 2 (Mbd2), (10ug)
"NM_010773" in other vectors (2)
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Frequently bought together (3)
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Mbd2 |
Synonyms | MBD2a |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC046607
GTGCGCGCCCGCTCTATGATGCTTGCGCGCGTCCCCCGCGCGCCGCTCTGCGGGCGGGGCGGGTCTCCGG GATTCCAAGGGCTCGGTTACGGAAGAAGCGCAGAGCCGGCTGGGGAGGGGGCTGGATGCGCGCGCACCCG GGGGGAGGCCGCTGCTGCCCGGAGCAGGAGGAGGGGGAGAGCGCGGCGGGCGGCAGCGGCGCTGGCGGCG ACTCCGCCATAGAGCAGGGGGGCCAGGGCAGCGCGCTCGCTCCGTCCCCGGTGAGCGGCGTGCGCAGGGA AGGCGCTCGGGGCGGCGGCCGTGGCCGGGGGCGGTGGAAGCAGGCGGCCCGGGGCGGCGGCGTCTGTGGC CGTGGCCGTGGCCGTGGCCGGGGTCGGGGCCGTGGCCGGGGCCGGGGCCGGGGCCGCGGCCGTCCCCAGA GTGGCGGCAGCGGCCTTGGCGGCGACGGCGGCGGCGGCGCGGGCGGCTGCGGCGTCGGCAGCGGTGGCGG CGTCGCCCCCCGGCGGGATCCTGTCCCTTTCCCGTCGGGGAGCTCGGGGCCGGGGCCCAGGGGACCCCGG GCCACGGAGAGCGGGAAGAGGATGGACTGCCCGGCCCTCCCCCCCGGATGGAAGAAGGAGGAAGTGATCC GAAAATCAGGGCTCAGTGCTGGCAAGAGCGATGTCTACTACTTCAGTCCAAGTGGTAAGAAGTTCAGAAG TAAACCTCAGCTGGCAAGATACCTGGGAAATGCTGTTGACCTTAGCAGTTTTGACTTCAGGACCGGCAAG ATGATGCCTAGTAAATTACAGAAGAACAAGCAGAGACTCCGGAATGACCCCCTCAATCAGAACAAGGGTA AACCAGACCTGAACACAACATTGCCAATTAGACAAACTGCATCAATTTTCAAGCAACCAGTAACCAAATT CACGAACCACCCGAGCAATAAGGTGAAGTCAGACCCCCAGCGGATGAATGAACAACCACGTCAGCTTTTC TGGGAGAAGAGGCTACAAGGACTTAGCGCATCAGATGTAACAGAACAAATTATAAAAACCATGGAGCTAC CTAAAGGTCTTCAAGGAGTCGGTCCAGGTAGCAATGACGAGACCCTTCTGTCTGCTGTGGCCAGTGCTTT ACACACAAGCTCTGCGCCCATCACAGGACAAGTCTCTGCTGCCGTGGAAAAGAACCCTGCTGTTTGGCTT AACACATCTCAACCCCTCTGCAAAGCTTTCATTGTTACAGATGAAGACATTAGGAAACAGGAAGAGCGAG TCCAACAAGTACGCAAGAAACTGGAGGAGGCACTGATGGCCGACATCCTGTCCCGGGCTGCGGACACGGA GGAAGTAGACATTGACATGGACAGTGGAGATGAGGCGTAAGAATATGATCAGGTAACTTTCGACTGACCT TCCCCAAGAGCAAATTGCTAGAAACAGAATTAAAACATTTCCACTGGGTTTCGCCTGTAAGAAAAAGTGT ACCTGAGCACATAGCTTTTTAATAGCACTAACCAATGCCTTTTTAGATGTATTTTTGATGTATATATCTA TTATTCCAAATGATGTTTATTTTGAATCCTAGGACTTAAAATGAGTCTTTTATAATAGCAAGCAGGGCCC TTCCGGTGCAGTGCAGCTTTGAGGCCAGGTGCAGTCTACTGGAAAGGTAGCACTTACGTGAAATATTTGT TTCCCCCACAGTTTTAATATAAACAGATCAGGAGTACCAAATAAGTTTCCCAATTAAAGATTATTATACT TCACTGTATATAAACAGATTTTTATACTTTATTGAAAGAAGATACCTGTACATTCTTCCATCATCACTGT AAAGACAAATAAATGACTATATTCACAGACAAAAAAAAAAAAAAA |
Restriction Sites | RsrII-NotI |
ACCN | NM_010773 |
Insert Size | 1245 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC046607, AAH46607 |
RefSeq Size | 1865 bp |
RefSeq ORF | 1245 bp |
Locus ID | 17191 |
UniProt ID | Q9Z2E1 |
Cytogenetics | 18 44.54 cM |
Gene Summary | Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression (By similarity). May enhance the activation of some unmethylated cAMP-responsive promoters (By similarity). Selectively represses transcription activity of methylated rRNA promoters.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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