Ska1 (NM_025581) Mouse Untagged Clone

CAT#: MC203855

Ska1 (untagged) - Mouse spindle and kinetochore associated complex subunit 1 (Ska1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ska1
• Synonyms: 2810433K01Rik; AV117428
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC019940
  TCGACCCACGCGTCCGCCCACGCGTCCGCGCAAGGTCGCTGGCTCCGAAGCCGCCCGAAGAGGCATCGCT TAGCAGGTTCCGGGAGCGGGCGACTCGGAGGGTTGGCCCCCGCCTTCCCCGTTCCAGCCTGCGCAAAATC ACGCTTAAAGGATGGACTCAGAGCTAGAAGACTTATGCTCTTATGTTAATGAGAAGATTGGAAATATTAA GAAAATTCTGTCGATAAGAAATCTTGGCCAGGACCCTGCCTTGAAGACCACCTTAAGTAAAATAGGAGAT GAGATCATTGCAGTAAATGAGCTTCTAAATAAATTTGAATTGGAAATTCAGTATCAAGAACAAACTAACA GTTCACTCAAGGAGCTCTGTGAATCTCTTAGAGAAGAGTGTGAAGATGTGGAACATCTCAAAGAACACGT CCCTCCCCATCTGCCTCAAGTGACGGCGACCCAGAGCTTAGTTCACAAGCCCGAGCCGGATCCTAAAGAG TCAGACAAAGCTGAGGAGCCTGGACTGCCGAAGAAGCCCCCCAGAGAGCAGAGAGTTATCAAGGAAATGC AATTTATAACTATGGATGAATTCAGTGACGTTCCTGCGTACATGAAATCCCGTTTAACATATTGTCAAAT TAATGATATTATTAAGGAAATTAACAAGGCAGTAGTTAGCAAATATAAGATTATGCATCAACCAAAAGCA TCTATGAGCTCTGTGAAGAGAAATCTCTACCAGAGATTTATTAATGAAGAAACGAAGGACACGAAAGGTC ATCACTTCATCGTGGAAGCTGACATAAAGGAATTCACGGCTTTGAAAGTTGACAAGAGGTTTTATGTGAT CATGCACATTTTGCGGCATTGCCACAGACTGTCAGAGGTCCGAGGGGGAGGACTTACCCGATACGTCATC ACCTGAGGCTTTTGAAGACTCGGGACTGATTGGCAGCAGGGTATCGAGGGGGGTCTTGAAATTTCATGTA TGTTTTTAGCTTCTCCATTTCCTGGCCTTGTTTGTTACAGAAGTAAAACTTTGGATAAAGCATATATATT TTCAAAACGTACTGCCTTTAGGAACGTGGGTGAAGCTGGAGCTCACAAGCACAGTAAGCCAGTTAGGAAG ACTGACTCGTGTGGAACCTGTGTGTGTATAGGAGTCAGGGGATCATGAGGCCAGGAGGAGGATCTGGAAA AGTGCGGAGAAAAGAGGAACAGAGAAGGCAGTGGACCGCTGTGATAGAGCAGGAGGAAAGACCGTCTGGG AACAGCCAGGGGAAGGGTGGCAAGCACTTGGGCAGGAGGTGGATCAGGATAAAGTGCGATGCCACAAGAT ATAAAAATGCAGTAACAGGACTCATTTCTTTGTGTGCTAACATAAAAATTAATTTTAAAAATCCCTGCCT TTCAGCAAATATTCAGGCACTGCCCTGTCAAGCCTCTGCATGAGTTGTATAATGGACAGCGTGTCTAGTT CTGTTAAGTGCAGATACCATGAGCATAGTATGAGGTCGGGAAAGATGGTGGATACCCAGGTCCTCTGTGG CGTAAAGTCTGATTACTGTTTTTCCACGTGCCCATAGAGAACAGCTTGCCTAAAATGCATTCAGTATAAG CTGGAGGCTCCGAAGTACATTGAGATACTGTCACATGTATTACATATGATATGTATTACAGCTTTACGTC TGGTTCCTTGTTCAGGGTTTCGAAAAATCCTTGTAACGTCCTAAGTGGTAAGGGTGCGTCTTGTGTTGAA CTCAGTTCTTGACTGTAGCTTTCAAACGCCTTTGAAACATTTTTCTGATCAGAACATCTTTTGCTCAGAT TCTAGTGAGGTGATTCTTGGGCGCCTTCAAGGCGGGCACTAGTTACCAAAATACTAAGCCATGACTAGGA AGTTGCAGTTTGGAGCCATTCTCCTGCTCCCGGGGAGGGAGACACCCTTGTTGCACATCCGGCCTCAGCA ACTCAGTTACAGGGGGAGATTTCTTGTCTCGAAAGCCAAAACCACTTGGTCAAAGCTGTCAAGTTCTGCT GCCTGATGAGGCTAGAGATGTTTGGTCATGCCGACAGGCTTCGTCCTTTTTAAAATTAATTTAAAAAAAT CACTGCTCTGGGCCTCCATGCAGGGACACCCCTGTCACACATCTGGCCTCAGCAACTTAGTTGCAGAGAG AGAGATTTCTTGACTCTAAAGCCAGAACCACATGGCTAAAGCTGCCAAGTTCTGTTGCTTTCTGGGGCTG GAGTATGGCCCTGTTGTTCAGTTAGATCTTCACCAGCGGTCTGGTTTTGATAGTTTCCTTCACTGCCTAA GCATGGCTGTCTTGGAACTTGCTCTGTAGACCAGGCTGCTCTTGAACTCAGAGATCTGCATACCCCTGTC TTCTAAGTGCTGGAACTAAAGGCGTGTACCACCCACCACACCTGGACCTAAACTTTCCCTTAATTCCTTT TCATTTTACATCGTTGTAAGCATGGCCACTGTGCCTCACGATATGGATTAAAGGTTCGTGTTCATCCACA AAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_025581
• Insert Size: 765 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019940, AAH19940
• RefSeq Size: 2530 bp
• RefSeq ORF: 765 bp
• Locus ID: 66468
• UniProt ID: Q9CPV1
• Cytogenetics: 18 E2
• Gene Summary: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.