Human TMIE (NM_147196) AAV Particle

CAT#: RC220050A1V

AAV ORF Particles, serotype AAV-2, TMIE (Myc-DDK-tagged)-Human transmembrane inner ear (TMIE), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: TMIE
• Synonyms: DFNB6
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220050 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGTGGCCGGGCGCGGGTCCCCTCTGCGTGCTGGGCGGCGCCGCACTCGGGGTGTGCCTCGCGG
  GGGTTGCCGGGCAGCTGGTGGAGCCCAGCACGGCCCCACCCAAGCCCAAGCCGCCTCCGCTGACCAAGGA
  GACAGTGGTGTTCTGGGACATGCGCCTGTGGCACGTGGTGGGCATCTTTTCGCTCTTCGTGTTGTCCATC
  ATCATCACGCTGTGCTGTGTCTTCAACTGTCGTGTGCCACGGACCCGGAAGGAGATCGAAGCCCGGTACC
  TGCAGCGAAAGGCAGCCAAGATGTACACAGACAAGCTGGAGACTGTGCCACCCCTCAATGAGCTCACAGA
  AGTCCCAGGAGAGGATAAGAAGAAGAAGAAGAAGAAGAAGGACAGTGTGGACACAGTGGCCATCAAAGTA
  GAGGAGGATGAGAAGAATGAGGCCAAGAAGAAGAAAGGAGAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220050 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGWPGAGPLCVLGGAALGVCLAGVAGQLVEPSTAPPKPKPPPLTKETVVFWDMRLWHVVGIFSLFVLSI
  IITLCCVFNCRVPRTRKEIEARYLQRKAAKMYTDKLETVPPLNELTEVPGEDKKKKKKKKDSVDTVAIKV
  EEDEKNEAKKKKGEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_147196
• ORF Size: 465 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_147196.2
• RefSeq Size: 1861 bp
• RefSeq ORF: 471 bp
• Locus ID: 259236
• UniProt ID: Q8NEW7
• Cytogenetics: 3p21.31
• MW: 17.1 kDa