Human CASQ1 (NM_001231) AAV Particle

CAT#: RC204696A1V

AAV ORF Particles, serotype AAV-2, CASQ1 (Myc-DDK-tagged)-Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: CASQ1
• Synonyms: CASQ; PDIB1; VMCQA
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204696 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCAGAGCTGTGCCGGGTCTGCGGCTGGCACTGCTGTTGCTGCTGGTGCTAGGGACACCCAAGT
  CAGGGGTACAGGGGCAGGAAGGGCTGGACTTCCCTGAGTACGATGGTGTGGACCGTGTGATCAATGTCAA
  TGCAAAGAACTACAAGAATGTGTTCAAGAAGTATGAGGTGCTGGCACTCCTCTACCATGAACCCCCCGAG
  GATGACAAGGCCTCACAAAGACAATTTGAGATGGAGGAGCTGATCCTGGAGTTAGCAGCCCAAGTCCTAG
  AAGACAAGGGTGTTGGCTTCGGGCTGGTAGACTCTGAGAAGGATGCAGCTGTGGCCAAGAAACTAGGCCT
  AACTGAAGTGGACAGCATGTATGTATTCAAGGGAGATGAAGTCATTGAGTACGATGGCGAGTTTTCTGCT
  GACACCATCGTGGAGTTTCTGCTTGATGTCCTAGAGGACCCTGTGGAATTGATTGAAGGTGAACGAGAGC
  TGCAGGCGTTTGAGAATATTGAGGATGAGATCAAACTCATTGGCTACTTCAAGAGCAAAGACTCAGAGCA
  TTACAAAGCCTTCGAGGATGCAGCTGAGGAGTTTCATCCCTACATCCCCTTCTTCGCCACCTTCGACAGC
  AAGGTGGCAAAGAAGCTGACCCTGAAGCTGAATGAGATTGATTTCTACGAGGCCTTCATGGAAGAGCCTG
  TGACCATCCCAGACAAGCCCAATAGCGAAGAGGAGATTGTCAACTTCGTGGAGGAGCACAGGAGATCAAC
  CCTGAGGAAACTGAAGCCGGAGAGTATGTATGAGACCTGGGAGGATGATATGGATGGAATCCACATTGTG
  GCCTTCGCAGAGGAAGCTGATCCTGATGGTTTCGAGTTCTTAGAGACTCTCAAGGCTGTGGCCCAAGATA
  ACACTGAAAACCCAGATCTTAGCATCATCTGGATTGACCCTGATGACTTCCCCCTGCTGGTCCCATACTG
  GGAGAAGACGTTTGACATCGACTTGTCAGCCCCACAAATAGGAGTCGTCAATGTTACTGATGCGGATAGC
  GTATGGATGGAAATGGACGATGAGGAGGACCTGCCTTCTGCTGAGGAGCTGGAGGACTGGCTGGAGGATG
  TCCTGGAGGGCGAGATCAACACAGAGGACGATGACGATGATGATGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204696 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPRAVPGLRLALLLLLVLGTPKSGVQGQEGLDFPEYDGVDRVINVNAKNYKNVFKKYEVLALLYHEPPE
  DDKASQRQFEMEELILELAAQVLEDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSA
  DTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDAAEEFHPYIPFFATFDS
  KVAKKLTLKLNEIDFYEAFMEEPVTIPDKPNSEEEIVNFVEEHRRSTLRKLKPESMYETWEDDMDGIHIV
  AFAEEADPDGFEFLETLKAVAQDNTENPDLSIIWIDPDDFPLLVPYWEKTFDIDLSAPQIGVVNVTDADS
  VWMEMDDEEDLPSAEELEDWLEDVLEGEINTEDDDDDDDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001231
• ORF Size: 1170 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001231.3, NP_001222.2
• RefSeq Size: 1993 bp
• RefSeq ORF: 1191 bp
• Locus ID: 844
• UniProt ID: P31415
• Cytogenetics: 1q23.2
• MW: 44.5 kDa