Human BRAF35 (HMG20B) (NM_006339) AAV Particle

CAT#: RC202444A1V

AAV ORF Particles, serotype AAV-2, HMG20B (Myc-DDK-tagged)-Human high mobility group 20B (HMG20B), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: BRAF35
• Synonyms: BRAF25; BRAF35; HMGX2; HMGXB2; PP7706; pp8857; SMARCE1r; SOXL
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202444 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCACGGCCCCAAGCAGCCCGGCGCGGCCGCCGCGCCGGCGGGCGGCAAGGCTCCGGGCCAGCATG
  GGGGCTTCGTGGTGACTGTCAAGCAAGAGCGCGGCGAGGGTCCACGCGCGGGCGAGAAGGGGTCCCACGA
  GGAGGAGCCGGTGAAGAAACGCGGCTGGCCCAAGGGCAAGAAGCGGAAGAAGATTCTGCCGAATGGGCCC
  AAGGCACCGGTCACGGGCTACGTGCGCTTCCTGAACGAGCGGCGCGAGCAGATCCGCACGCGCCACCCGG
  ATCTGCCCTTTCCCGAGATCACCAAGATGCTGGGCGCCGAGTGGAGCAAGCTGCAGCCAACGGAAAAGCA
  GCGGTACCTGGATGAGGCCGAGAGAGAGAAGCAGCAGTACATGAAGGAGCTGCGGGCGTACCAGCAGTCT
  GAAGCCTATAAGATGTGCACGGAGAAGATCCAGGAGAAGAAGATCAAGAAAGAAGACTCGAGCTCTGGGC
  TCATGAACACTCTCCTGAATGGACACAAGGGTGGGGACTGCGATGGCTTCTCCACCTTCGATGTTCCCAT
  CTTCACTGAAGAGTTCTTGGACCAAAACAAAGCGCGTGAGGCGGAGCTTCGGCGCTTGCGGAAGATGAAT
  GTGGCCTTCGAGGAGCAGAACGCGGTACTGCAGAGGCACACGCAGAGCATGAGCAGCGCGCGCGAGCGTC
  TGGAGCAGGAGCTGGCGCTGGAGGAGCGGAGGACGCTGGCGCTGCAGCAGCAGCTCCAGGCCGTGCGCCA
  GGCGCTCACCGCCAGCTTCGCCTCACTGCCGGTGCCGGGCACGGGCGAAACGCCCACGCTGGGCACTCTG
  GACTTCTACATGGCCCGGCTTCACGGAGCCATCGAGCGCGACCCCGCCCAGCACGAGAAGCTCATCGTCC
  GCATCAAGGAAATCCTGGCCCAGGTCGCCAGCGAGCACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202444 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHGPKQPGAAAAPAGGKAPGQHGGFVVTVKQERGEGPRAGEKGSHEEEPVKKRGWPKGKKRKKILPNGP
  KAPVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQS
  EAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMN
  VAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTL
  DFYMARLHGAIERDPAQHEKLIVRIKEILAQVASEHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_006339
• ORF Size: 951 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_006339.2
• RefSeq Size: 1584 bp
• RefSeq ORF: 954 bp
• Locus ID: 10362
• UniProt ID: Q9P0W2
• Cytogenetics: 19p13.3
• MW: 35.8 kDa