Mouse Pnpla2 (NM_001163689) AAV Particle

CAT#: MR226260A1V

AAV ORF Particles, serotype AAV-2, Pnpla2 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 1, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Pnpla2
• Synonyms: 0610039C21Rik; 1110001C14Rik; Atgl; TTS-2.2
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226260 representing NM_001163689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCGAGGGAGACCAAGTGGAACATCTCATTCGCTGGCTGCGGCTTCCTCGGGGTCTACCACATTG
  GCGTGGCCTCCTGCCTCCGTGAGCACGCGCCCTTCCTGGTGGCCAACGCCACTCACATCTACGGAGCCTC
  GGCAGGGGCGCTCACCGCCACAGCGCTGGTCACTGGGGCCTGCCTGGGTGAAGCAGGTGCCAACATTATT
  GAGGTGTCCAAGGAGGCCCGGAAGCGGTTCCTGGGTCCTCTGCATCCCTCCTTCAACCTGGTGAAGACCA
  TCCGTGGCTGTCTACTAAAGACCCTGCCTGCTGATTGCCATGAGCGCGCCAATGGACGCCTGGGCATCTC
  CCTGACTCGTGTTTCAGACGGAGAGAACGTCATCATATCCCACTTTAGCTCCAAGGATGAGCTCATCCAG
  GCCAATGTCTGCAGCACATTTATCCCGGTGTACTGTGGCCTCATTCCTCCTACCCTCCAAGGGGTGCGCT
  ATGTGGATGGCGGCATTTCAGACAACTTGCCACTTTATGAGCTGAAGAATACCATCACAGTGTCCCCATT
  CTCAGGCGAGAGTGACATCTGCCCTCAGGACAGCTCCACCAACATCCACGAGCTTCGCGTCACCAACACC
  AGCATCCAGTTCAACCTTCGCAATCTCTACCGCCTCTCGAAGGCTCTCTTCCCGCCAGAGCCCATGGTCC
  TCCGAGAGATGTGCAAACAGGGCTACAGAGATGGACTTCGATTCCTTAGGAGGAATGGCCTACTGAACCA
  ACCCAACCCTTTGCTGGCACTGCCCCCAGTTGTCCCCCAGGAAGAGGATGCAGAGGAAGCTGCTGTGGTG
  GAGGAGAGGGCTGGAGAGGAGGATCAATTGCAGCCTTATAGAAAAGATCGAATTCTAGAGCACCTGCCTG
  CCAGACTCAATGAGGCCCTGCTGGAGGCCTGTGTGGAACCAAAGGACCTGATGACCACCCTTTCCAACAT
  GCTACCAGTGCGCCTGGCAACGGCCATGATGGTGCCCTATACTCTGCCGCTGGAGAGTGCAGTGTCCTTC
  ACCATCCGCTTGTTGGAGTGGCTGCCTGATGTCCCTGAAGATATCCGGTGGATGAAAGAGCAGACGGGTA
  GCATCTGCCAGTATCTGGTGATGAGGGCCAAGAGGAAATTGGGTGGCCATCTGCCTTCCAGACTGTCTGA
  GCAGGTGGAACTGCGACGTGCCCAGTCTCTGCCCTCTGTGCCACTGTCTTGCGCCACCTACAGTGAGGCC
  CTACCCAACTGGGTACGAAACAACCTCTCACTGGGGGACGCGCTGGCCAAGTGGGAAGAATGCCAGCGTC
  AGCTACTGCTGGGTCTCTTCTGCACCAATGTGGCCTTCCCGCCGGATGCCTTGCGCATGCGCGCACCTGC
  CAGCCCCACTGCCGCAGATCCTGCCACCCCACAGGATCCACCTGGCCTCCCGCCTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226260 representing NM_001163689
  Red=Cloning site Green=Tags(s)
  
  MFPRETKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEAGANII
  EVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGENVIISHFSSKDELIQ
  ANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNT
  SIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNGLLNQPNPLLALPPVVPQEEDAEEAAVV
  EERAGEEDQLQPYRKDRILEHLPARLNEALLEACVEPKDLMTTLSNMLPVRLATAMMVPYTLPLESAVSF
  TIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGGHLPSRLSEQVELRRAQSLPSVPLSCATYSEA
  LPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPATPQDPPGLPPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_001163689
• ORF Size: 1458 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_001163689.1, NP_001157161.1
• RefSeq Size: 2635 bp
• RefSeq ORF: 1461 bp
• Locus ID: 66853
• UniProt ID: Q8BJ56
• Cytogenetics: 7 F5
• MW: 54.1 kDa