Mouse Chrna7 (NM_007390) AAV Particle

CAT#: MR224522A1V

AAV ORF Particles, serotype AAV-2, Chrna7 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 7 (Chrna7), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Chrna7
• Synonyms: Acra7; alpha7; nAchR
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224522 representing NM_007390
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGGCCGGCGGGGAGGCATCTGGCTGGCTCTGGCCGCGGCGCTGCTGCACGTGTCCCTGCAAGGCG
  AGTTCCAGAGGAGGCTGTACAAGGAGCTGGTCAAGAACTACAACCCGCTGGAGAGGCCGGTGGCCAACGA
  CTCGCAGCCGCTCACCGTGTACTTCTCCCTGAGCCTCCTGCAGATCATGGATGTGGATGAGAAGAACCAA
  GTTTTAACCACCAACATTTGGCTACAAATGTCTTGGACAGATCACTATTTGCAGTGGAACATGTCTGAGT
  ACCCCGGAGTGAAAAATGTTCGTTTTCCAGATGGCCAGATTTGGAAACCAGACATTCTCCTCTATAACAG
  TGCAGATGAACGCTTTGATGCCACATTCCACACCAACGTCTTGGTGAATGCATCTGGGCATTGCCAGTAT
  CTCCCTCCAGGCATATTCAAGAGCTCCTGCTACATCGATGTACGCTGGTTCCCTTTTGATGTGCAGCAGT
  GCAAACTGAAGTTTGGGTCCTGGTCCTATGGAGGGTGGTCCTTGGACCTGCAGATGCAAGAGGCAGATAT
  CAGCAGCTATATCCCCAATGGAGAATGGGATCTCATGGGAATCCCTGGCAAAAGGAATGAGAAGTTCTAT
  GAATGCTGCAAAGAGCCATACCCAGATGTCACCTACACAGTAACCATGCGCCGTAGGACACTCTACTATG
  GCCTCAACCTGCTCATTCCTTGTGTGCTCATTTCAGCCCTGGCTTTGCTGGTATTCTTGCTGCCTGCAGA
  CTCTGGAGAGAAAATCTCTCTTGGAATAACTGTCTTACTTTCTCTGACTGTCTTCATGCTGCTTGTGGCT
  GAGATCATGCCAGCAACATCTGATTCCGTGCCCTTGATAGCACAGTACTTCGCCAGCACCATGATCATCG
  TGGGCCTCTCAGTGGTCGTGACAGTGATTGTGCTGCGATATCACCACCATGACCCTGATGGTGGCAAAAT
  GCCTAAGTGGACCAGGATCATTCTTCTGAATTGGTGTGCATGGTTTCTGCGCATGAAGAGGCCGGGAGAG
  GACAAGGTGCGGCCGGCTTGTCAGCACAAGCCTCGGCGCTGCAGCCTGGCCAGTGTGGAGCTGAGCGCAG
  GTGCGGGGCCACCCACCAGCAATGGCAACCTGCTCTACATTGGCTTCCGAGGCCTGGAGGGCATGCACTG
  TGCCCCAACTCCAGACTCTGGGGTCGTGTGTGGTCGTTTGGCCTGCTCCCCAACACATGATGAGCACCTC
  ATGCATGGTACACACCCCTCTGATGGGGACCCCGACCTGGCCAAGATCCTGGAGGAGGTCCGCTACATCG
  CCAACCGCTTCCGCTGCCAGGATGAGAGTGAGGTGATCTGCAGTGAGTGGAAGTTTGCGGCCTGTGTGGT
  GGACCGCTTGTGCCTCATGGCCTTTTCGGTTTTTACCATCATCTGCACCATCGGCATCCTCATGTCAGCC
  CCAAACTTTGTGGAGGCTGTGTCCAAAGACTTTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224522 representing NM_007390
  Red=Cloning site Green=Tags(s)
  
  MCGRRGGIWLALAAALLHVSLQGEFQRRLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQ
  VLTTNIWLQMSWTDHYLQWNMSEYPGVKNVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNASGHCQY
  LPPGIFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQMQEADISSYIPNGEWDLMGIPGKRNEKFY
  ECCKEPYPDVTYTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVA
  EIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLRYHHHDPDGGKMPKWTRIILLNWCAWFLRMKRPGE
  DKVRPACQHKPRRCSLASVELSAGAGPPTSNGNLLYIGFRGLEGMHCAPTPDSGVVCGRLACSPTHDEHL
  MHGTHPSDGDPDLAKILEEVRYIANRFRCQDESEVICSEWKFAACVVDRLCLMAFSVFTIICTIGILMSA
  PNFVEAVSKDFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_007390
• ORF Size: 1506 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_007390.3, NP_031416.3
• RefSeq Size: 2091 bp
• RefSeq ORF: 1509 bp
• Locus ID: 11441
• UniProt ID: P49582
• Cytogenetics: 7 34.47 cM
• MW: 57.1 kDa