Mouse Gtf2a1 (NM_031391) AAV Particle

CAT#: MR223406A1V

AAV ORF Particles, serotype AAV-2, Gtf2a1 (Myc-DDK-tagged) - Mouse general transcription factor II A, 1 (Gtf2a1), transcript variant 1, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Gtf2a1
• Synonyms: 6330549H03Rik; AA536742; AA959775; AW060250; Tfiia1; TfIIAa/b
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223406 representing NM_031391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACTCGGCAAACACAAACACCGTGCCTAAATTGTATAGATCTGTGATTGAAGATGTTATTAATG
  ACGTACGAGACATTTTTCTGGATGACGGAGTGGATGAACAAGTCCTGATGGAACTAAAAACTTTGTGGGA
  GAATAAACTAATGCAGTCTAGGGCAGTAGATGGATTTCATTCAGAAGAACAGCAGCTTTTGTTGCAAGTT
  CAGCAGCAGCATCAACCCCAGCAGCAGCAGCATCACCACCACCACCACCAGCACCAGCAAGCGCAACCTC
  AGCAAACGGTACCTCAGCAAGCACAGACCCAGCAGGTCCTCATTCCTGCATCCCAGCAAGCTACAGCACC
  ACAAGTTATTGTTCCTGATTCTAAGTTGCTACAACATATGAATGCATCAAGTATTACGAGTGCTGCTGCT
  ACAGCTGCTACCTTGGCACTGCCTGCAGGAGTGACTCCGGTTCAACAGCTACTAACAAATTCAGGCCAGC
  TGCTTCAGGTGGTCAGAGCAGCCAATGGTGCCCAGTATATCTTGCAGCCTCAGCAGTCAGTTGTTCTACA
  ACAACAAGTTATACCACAAATGCAGCCTGGTGGAGTCCAAGCTCCTGTTATCCAGCAGGTCTTAGCCCCA
  CTTCCTGGAGGGATTTCACCACAGACAGGCGTCATCATCCAGCCACAGCAAATCTTATTTACAGGAAATA
  AGACTCAGGTCATTCCTACAACAGTGGCAGCACCCGCCCCGGCACAAGCGCCGATGCCTGCAGCCGGACA
  GCAGCAGCCACAGGCCCAGCCTGCCCAACAGCAAGCTCCGTTGGTGCTGCAAGTTGATGGAACAGGGGAT
  ACATCTTCTGAGGAAGATGAAGATGAAGAAGAAGACTATGATGATGATGAGGAAGAAGATAAAGAGAAAG
  ATGGAGCTGAAGATGGGCAGGTGGAAGAAGAGCCGCTCAACAGCGAGGATGACGTCAGTGACGAGGAAGG
  GCAGGAACTCTTTGACACTGAGAATGTTGTCGTCTGCCAGTATGATAAGATACACAGAAGTAAAAATAAA
  TGGAAATTTCATCTCAAGGATGGCATTATGAATCTTAACGGAAGAGATTATATATTTTCCAAAGCCATTG
  GAGATGCAGAGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223406 representing NM_031391
  Red=Cloning site Green=Tags(s)
  
  MANSANTNTVPKLYRSVIEDVINDVRDIFLDDGVDEQVLMELKTLWENKLMQSRAVDGFHSEEQQLLLQV
  QQQHQPQQQQHHHHHHQHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIVPDSKLLQHMNASSITSAAA
  TAATLALPAGVTPVQQLLTNSGQLLQVVRAANGAQYILQPQQSVVLQQQVIPQMQPGGVQAPVIQQVLAP
  LPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPAPAQAPMPAAGQQQPQAQPAQQQAPLVLQVDGTGD
  TSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNK
  WKFHLKDGIMNLNGRDYIFSKAIGDAEW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_031391
• ORF Size: 1134 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_031391.2, NP_113568.2
• RefSeq Size: 5704 bp
• RefSeq ORF: 1137 bp
• Locus ID: 83602
• UniProt ID: Q99PM3
• Cytogenetics: 12
• MW: 41.6 kDa