Mouse Dbt (NM_010022) AAV Particle

CAT#: MR207721A1V

AAV ORF Particles, serotype AAV-2, Dbt (Myc-DDK-tagged) - Mouse dihydrolipoamide branched chain transacylase E2 (Dbt), nuclear gene encoding mitochondrial protein, 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Dbt
• Synonyms: BCKAD-E2; D3Wsu60e
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207721 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGCGCGTGTACTGAGGACCTGGAGCCAGAACGCTGTCAGACTGACCTGTGTTCGCTATTTTC
  AGACATTTAATAGCGCCCGTGTTTTGAAGCCAAAATGTGTGTGCTCTGTGGGTTATCCATTGTTCAAGTA
  CAGTCAGCCACGTCACTCCCTGAGAACAGCGGCCGTGCTCCAGGGACAGGTTGTTCAGTTCAAACTCTCC
  GACATCGGAGAAGGAATTCGAGAAGTAACTATTAAAGAATGGTATGTAAAAGAAGGAGATACGGTGTCTC
  AGTTTGACAGCATCTGTGAAGTTCAAAGTGACAAGGCCTCTGTCACCATCACCAGCCGTTACGATGGCAT
  CATTAAGAGGCTCTATTACAATCTAGATGATATCGCTTATGTGGGGAAACCACTGATCGACATTGAGACA
  GAAGCTTTAAAGGATTCAGAGGAAGATGTTGTTGAAACCCCCGCTGTGTCCCATGATGAGCACACTCACC
  AAGAGATAAAAGGCCAGAAAACACTAGCAACGCCTGCAGTGCGTCGGCTGGCAATGGAAAACAATATTAA
  GCTGAGTGAAGTTGTTGGCTCAGGAAAAGATGGCAGAATACTTAAAGAAGATATTCTCAGCTTTTTGGAA
  AAGCAGACAGGAGCCATACTGCCTCCCTCACCAAAATCTGAAATCACACCACCTCCACCACAGCCCAAAG
  ACAGGACCTTTCCCACGCCGATAGCAAAGCCTCCAGTGTTCACAGGCAAAGACCGAACAGAGCCAGTGAC
  AGGCTTCCAGAAGGCAATGGTGAAGACCATGTCTGCAGCCCTGAAGATTCCCCACTTCGGGTATTGTGAT
  GAGATTGACCTTACTCAACTGGTTAAGCTTCGAGAAGAGCTGAAACCTGTTGCTTTGGCGCGTGGAATCA
  AACTCTCCTTCATGCCCTTCTTCTTAAAGGCTGCTTCTCTGGGACTCCTGCAGTTTCCTATCCTCAATGC
  CTCCGTGGACGAGAACTGCCAGAACATCACCTACAAGGCTTCTCACAACATTGGGATAGCAATGGACACT
  GAGCTGGGGTTAATTGTCCCCAATGTGAAGAACGTTCAGGTCCGCTCTGTATTTGAGATCGCCATGGAAC
  TGAACCGCCTCCAGAAACTGGGCTCTTCCGGTCAGCTCGGTACCACCGACCTTACAGGAGGGACATTTAC
  TCTTTCCAACATTGGATCAATCGGTGGAACCTATGCCAAGCCAGTGATATTGCCACCGGAAGTAGCCATT
  GGTGCCCTGGGAGCGATTAAGGCTCTTCCCCGATTTGACCAGAAAGGAGACGTTTATAAAGCACAGATAA
  TGAATGTGAGCTGGTCAGCGGATCACAGGGTCATCGATGGGGCCACCATGTCACGCTTCTCTAATTTGTG
  GAAATCCTACTTAGAAAACCCAGCGTTTATGCTGCTGGATCTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207721 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAARVLRTWSQNAVRLTCVRYFQTFNSARVLKPKCVCSVGYPLFKYSQPRHSLRTAAVLQGQVVQFKLS
  DIGEGIREVTIKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGIIKRLYYNLDDIAYVGKPLIDIET
  EALKDSEEDVVETPAVSHDEHTHQEIKGQKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILSFLE
  KQTGAILPPSPKSEITPPPPQPKDRTFPTPIAKPPVFTGKDRTEPVTGFQKAMVKTMSAALKIPHFGYCD
  EIDLTQLVKLREELKPVALARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDT
  ELGLIVPNVKNVQVRSVFEIAMELNRLQKLGSSGQLGTTDLTGGTFTLSNIGSIGGTYAKPVILPPEVAI
  GALGAIKALPRFDQKGDVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_010022
• ORF Size: 1449 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_010022.1, NP_034152.1
• RefSeq Size: 3292 bp
• RefSeq ORF: 1449 bp
• Locus ID: 13171
• UniProt ID: P53395
• Cytogenetics: 3 50.37 cM
• MW: 53.3 kDa