Mouse Bsnd (NM_080458) AAV Particle

CAT#: MR204322A1V

AAV ORF Particles, serotype AAV-2, Bsnd (Myc-DDK-tagged) - Mouse Bartter syndrome, infantile, with sensorineural deafness (Barttin) (Bsnd), 250ul, >10^13 GC/mL

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: Bsnd
• Synonyms: MGC47291
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204322 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGATGAGAAGACCTTCCGAATCGGCTTCATCGTGCTGGGCCTCTTCCTGCTATCCCTGGGTACAT
  TCCTTATGAGCCACGATCGACCCCAGGTCTACGGCACCTTCTATGCCATGGGCAGCGTCATGGTGATCGG
  GGGTGTCATCTGGAGCATGTGCCAATGCTATCCTAAGATCACCTTTGTGCCAGCAGACTCTGACTTCCAA
  GGCATCTTGTCCCCAAAGGCCCTGAGCCTGCTGGAGACCGGGCTTTCGGAGGTAAAGAGCCCCCAGCCCC
  CCTACGTCAGGCTGTGGGAAGAAGCCGCCTATGACCAGAGCCTCCCAGACTTCACTCACATCCAAATGAA
  GGTCATGGGCTACAGTGAGGACCCCAGGCCACTGCTGGCCCCCGAGCTGAAGACAGGAGCCAGCAGTGTC
  AGGGAAGGCGAGCCTCGTACTGCTCAGGCCTGGATGGAGGCTCCGGTGGTCGTCCATAGAGGGTCAGATG
  AGAACGAGGGAGAGAAATCCCATTCTCAGAGCAGCCCTTCAGTGGGTCCCCAAGGCTCTGCACCCTTGGC
  CTCATTCCATGACGACCTGGACGTGGGCTCCAGCGAAGGCAGCAGCCTGCAACCATCCCCCAACAGGGAT
  GAGCCACACCGGCAGGTGCCCTGGGCCAGCAGGGGTCCACTGGATCGCTTCAGTGACTTTGCTCTGATTG
  ACGACACCCCTACATCTGAGGACACGGTCCTGGACGGGCAGGCACGGGAGGCAGCGCTACCCAGAAAGCA
  GCAATGGTCCCTGAGGATGAAGGGGGAGACTGTCCAGGCACGCGCAGAAGAACCGGAGCAGGAGGAGGAA
  GATCTGTATTACGGGCTGCCAGACAGCCCTGGGGACCCCCTACCCGACAAAGAGTTGGGCTTCGAGCCTG
  ACATCCAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204322 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADEKTFRIGFIVLGLFLLSLGTFLMSHDRPQVYGTFYAMGSVMVIGGVIWSMCQCYPKITFVPADSDFQ
  GILSPKALSLLETGLSEVKSPQPPYVRLWEEAAYDQSLPDFTHIQMKVMGYSEDPRPLLAPELKTGASSV
  REGEPRTAQAWMEAPVVVHRGSDENEGEKSHSQSSPSVGPQGSAPLASFHDDLDVGSSEGSSLQPSPNRD
  EPHRQVPWASRGPLDRFSDFALIDDTPTSEDTVLDGQAREAALPRKQQWSLRMKGETVQARAEEPEQEEE
  DLYYGLPDSPGDPLPDKELGFEPDIQG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Mouse
• Serotype: AAV-2
• ACCN: NM_080458
• ORF Size: 924 bp
• Storage Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.

Reference Data

• RefSeq: NM_080458.2, NP_536706.2
• RefSeq Size: 2716 bp
• RefSeq ORF: 924 bp
• Locus ID: 140475
• UniProt ID: Q8VIM4
• Cytogenetics: 4 C7
• MW: 33.8 kDa