TAF1 (NM_004606) Human 3' UTR Clone

CAT#: SC217283

3' UTR clone of TAF1 RNA polymerase II TATA box binding protein (TBP)-associated factor 250kDa (TAF1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TAF1
• Synonyms: BA2R; CCG1; CCGS; DYT3; DYT3/TAF1; KAT4; MRXS33; N-TAF1; NSCL2; OF; P250; TAF(II)250; TAF2A; TAFII-250; TAFII250; XDP
• ACCN: NM_004606
• Insert Size: 1989 bp
• Sequence Data:
  Insert Sequence

  >SC217283 3’UTR clone of NM_004606
  The sequence shown below is from the reference sequence of NM_004606. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGGGACAGTGACTTGGACTCTGATGAATGAGGCTTCCTTTGGGCCTCCTTGGTCAGCCTTCCCTGTTCT
  CCAGCCTAGGTGGTTCACCTTTCCCCAATTTGTTCATATTTGTACAGTATCTGATCCTGAAATCATGAA
  ATTAACTAACACCTTAGCCTTTTTAAAAGTAGTAAGTAAATGATAATAAATCACCTCTCCTAATCTTCC
  TGGGGCAATGTCACCCTTTGATTTAAAACAAAGCAACCCCCTTTCCCCTACCACTACGGAAAAGAGCAA
  GCTCATTTTTCCGTGTCCTCCTTTATTTAACTCCATTTATTGCTTTTGGTATAATTTTTCCCTGGGGAA
  GGAGGGGAAATTATGAAAGAACTAGTAACTTTATGTCCTCTTGATGTATTAGGAAATTTCCGGCCAGGC
  GTGGTGGCTCACACCTGTAATCTCAGCACTCTGGGAGGCCGAGGCGGGCAGATCACCTGAGGTCAGAAG
  TTCGAGACCAGCTTGGCCAACATGGCGAAACCGCATCTCTACTAAAAATACAAAAATTAGCCAGGTGTG
  GTGGCGTATGCCTGTTAATCCTAGCTACTCGGGAGGCTGAGGCAGGAGAATTACTTGAACCCGGGAGGC
  AGAGGTTGCAGTGAGTGGAGGTCACGCCACTGCACTCCAGCCTGGGCGAAAGAGTGAGATTCAGTCTCA
  AAAAAAAAAAAATTTCCAAGCATGGTATCATCTCACTTTTCTAATTTACAGGCTGGAGCAGATGAGAGC
  CCTCCTGCTGGGACAGAGAATTGGGTTCTAGTGGACTCTGTGCTACACTTAAACCTGTGAGACAAACCG
  CCCATTATTTTATTATTTAATTATGCAATGCCTAGTTCCTAAATGGATTGGAGGCAAATTACCGTAAAT
  TTTGAAACAGCCTATATGTCAGAAATGATAATGTTGCCACCTAAATGTTTTCTGTCCCCCCCACCCTCC
  CCAGGGGAAATGGTAGGAAAATGGTAAGTTTCTTAGGGCAAAGACTGTGTCTTCTGTTTCTTTTCATGC
  TTAGGATATGGTTCTGTGCATAGTAGGTACTCAGTAAATGTTCCTAGAATCATAAAATCCTCAACAGAT
  ATGTTACTGAGCATCTGCTTTTCATGATAAGCACTCTATCAGATCCTTGGGATGCAAAGGTAAATAAGA
  CAAATCCCTTTTACCCAAAGAGCTCACCATCAAGTTGGGGGAGGGAAAGTGGAATTCAAAACATGTTAA
  TAAATCATCATAGTACTGTGAGATAAGTGCAATTAAGAAGCTAGTTATAAAGTATAGGGGAAATAGAGG
  AGTAATCATGTCTGAAAAGTCAGGAAAGTCTTCCTAGAGGTAATTTTTAAGCTGATTGTTTTAGAATTA
  GTAGAAGCTTGCCAGATGGAAAAGTCCAGGCAAAGTGTAACATGAATGGGAAAGGCCACAGTCTAGAAA
  TGGCAGAGTGTGTTCCTAGTTTGTTTGTTTGTTTGTTTGTACCTGCCTTGTTCCAGGAAGGATTTAATG
  TGGTTTATATTCCAGTCCTTTAATGCTGGAAGGGCTGAGATGAGACTGAAAGATGGGCAGGAAGTATAT
  CATCACAAGCTTTGTGTTTGATGTTAATGTGTATGATTTTTATATTATGGGAAATAAGCTCTTAGAGGA
  GTGATATAATCAGGTTTGTGTTTTAGAAATCTGTGTAATGAATGAATGAAGAAAGAAATTGAAGAATCA
  TGTAACATATGTGATCGCATTTTTGTAAAAGAACCATGTGTGTTTATATGTGTTTATATATATACTTGT
  GTATGCAAAGGTAAAAGTCTGAAAGGATATATGCTAACTGTTCACAATGATAACCCCCCAGGAATGGGA
  TTGGAGGGGAGGGGGCTTCTGTGTTTGTTATGTATGCTGGGTGGGATATTGTGCTTTTATTTCTATATT
  GTTTGAATTTTTTTACAGTATGTATTATTTTTGTAATAAAAATTTTAAAAAATTCCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004606.5
• Summary: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]
• Locus ID: 6872
• MW: 75