ENAM (NM_031889) Human 3' UTR Clone

CAT#: SC217233

3' UTR clone of enamelin (ENAM) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ENAM
• Synonyms: ADAI; AI1C; AIH2
• ACCN: NM_031889
• Insert Size: 1999 bp
• Sequence Data:
  Insert Sequence

  >SC217233 3’UTR clone of NM_031889
  The sequence shown below is from the reference sequence of NM_031889. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GTACAAGACTGCTTACTACTTCAGGCCTAGGGGTTATCCAACCAAGCATTCTTGGGGAAAGAGAAATCA
  CTGACATTCTATACCAATGGTTCCCAAAATTTTTTCCCCAAGACTTAATTAAGTGTCTGACTGTCTTGG
  TGATCCTTAAGTTTTCTCCCCTCTCAGCAATAGGAGTAGCGACCCAGGTGGAGCTGAGATTAGGCCTCT
  GGGGATCACCAGATCTAGCACTTTCACAGATTAGTGCAGACCTTAAAAAAGCAAGAAGGCCATGACCAT
  CCCTGCCTCGAAACTTGCAAGTCACTTGTCTGAGTTAGTTTCCTTTTGCTTGATGACTTCTTTTGCTCT
  CAAAGTTCCATACTTGCAAAATTGGTTTTTCAAGACTGAAAGGCAGATTAACTTTCCATTCTACTTATG
  GAGATCCACACATCAGTATAGTCCTGATGCACTGTACGTTTTTTGCTTGTGTTGATCTTGTCAGGTTTT
  TTTCCTACTCAATATTCTCTTCAAGCATTTTTTTTCTTCCACTGCCACTCTCTTTACCTTTATCATTTC
  TTTTTCTTAGCTTTCTTCCTTCTTTTCTCTCTCCCTCAACCATTCTGTATTTAGCTTTCTAATCCTCAT
  CTTTTTCTCTCTGTATATCACTTCTTACATATTAAATTAGCTATTTTAACCATTTCTAGTCTCTTCTTT
  ATGTCTACCTCCTCCATTATGTCTTCGTCTCTTCCAACCTATCTGTTCTGTCTCCCAATGTACCACTTA
  TCAACTAATCCCTCTCCTCTCTGATTCCTTTCTTCCCTTCAATTTATCTTTTTCCCTGTTACACCATTT
  TCATCTTAAGACTGCATGTCACTCTCTTCCTTTTTGTATCCACTCTCCAAATCGTACCCTAAATTTTTC
  TTGACCCAGCTTAACCATCATTACAGAATATATTTTGGAAATGGTAGACACTGATAGCTACTTTGTTTT
  TAGTTATCTTAGGGTCTCTACCAAGTAGACACAGTTCAGTTAGAGAGACGTATTTAAGGAGAAGGGAAG
  GCAGGATACCCAGAACTAGCAGCCTAGGGAAAAGGCTTAGGAAGATGGAGAATGAGGAACTGAAGAAAC
  TGCCGCTCTGAGACTGCACATCCAGGAAAAAAGACTAGGACTTGCCACACACTGCCAATTCATAATCCG
  CAGGATCTGTAGAAGACATTTTGGGAAACACTGTCTTAAATAAAAACTCAATGTTTAGTTGTTTTTTTT
  TTTTTTTACTAGTTCAGCATAAGGGATTCTACCAGTATGTGCTACTACTAACATTCTTCATGTCCATGC
  AGAATTACTTACAGACACTCTTTCTTGGTATCTTGAATAAATTGTGTAAATTATTTTGAAAAGTGGATA
  TTTCTATATGCCTTGCTTATAATACATCAGGGATTATTATTCTTATTAATAACATGTTGGCCTATTATC
  TGGATGTCCCTTGCCTAGAGCAGGAAAAAAGTCTCAGAATTACCTAAAAACATACTTTATAACTTTAAG
  AGATTTCTTAGGATACATCTTCAGGGGCAGATTTCATAACAACATCTCAAAAATTTGGGGAAACACAAC
  GTTGAAAACTTCCTAAGAAAAGGAATCTTGCCCTTGAATCTTCTGAAGTACCTAGCACAGTGTCTTCAA
  CATAGAAGGTACCCAATAAATATATATCAATTCATTTTTTAATGACTTTCAACACTCCCATGAAAACAC
  CGTAGTCTGCTTTTCCTGAAAGGTGACTGTCTAGGATGCTGAATGAGCCACCACTCCGTAGCTTTATAT
  ATTCAGCAACATTTCCATCATAGAATTTCAGCGTTAGAAGGGATCTTAGAGGTAGATTATCCAGGCCAT
  CCCCTACTGCTTACATTCCCCTCCCCCTGGCCAGTTTTATATTTTAATCACACATTGAAAAATTCATTA
  ACTTATTCTGTATAAAACTGTCTTTTAAGAATTATCCTTTCAAAATAAAAATGTTATTGTTTCACTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_031889.3
• Summary: Dental enamel forms the outer cap of teeth and is the hardest substance found in vertebrates. This gene encodes the largest protein in the enamel matrix of developing teeth. The protein is involved in the mineralization and structural organization of enamel. Defects in this gene result in amelogenesis imperfect type 1C.[provided by RefSeq, Oct 2009]
• Locus ID: 10117
• MW: 77.7