KCNK2 (NM_001017425) Human 3' UTR Clone

CAT#: SC217169

3' UTR clone of potassium channel subfamily K member 2 (KCNK2) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNK2
• Synonyms: hTREK-1c; hTREK-1e; K2p2.1; TPKC1; TREK; TREK-1; TREK1
• ACCN: NM_001017425
• Insert Size: 1975 bp
• Sequence Data:
  Insert Sequence

  >SC217169 3’UTR clone of NM_001017425
  The sequence shown below is from the reference sequence of NM_001017425. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GAGATTGCTGTGATTGAGAACATCAAATAGCCCTCTCTTTAAATAACCTTAGGCATAGCCATAGGTGAG
  GACTTCTCTATGCTCTTTATGACTGTTGCTGGTAGCATTTTTTAAATTGTGCATGAGCTCAAAGGGGGA
  ACAAAATAGATACACCCATCATGGTCATCTATCATCAAGAGAATTTGGAATTCTGAGCCAGCACTTTCT
  TTCTGATGATGCTTGTTGAACGGTCCACTTTCTTTGATGAGTGGAATGACAAGCAATGTCTGATGCCTT
  TTTGTGCCCAGACTGTTTTCCTCTCTCTTTCCCTAATGTGCCATAAGGCCTCAGAATGAATGAGAATTG
  TTTCTGGTAACAATGTAGCTTTGAGGGATCAGTTCTTAACTTTTCAGGGTCTACCTAACTGAGCCTAGA
  TATGGACCATTTATGGATGACAACAATTTTTTTTTTGTAAATGACAAGAAATTCTTATGCAGCCTTTTA
  CCTAAGAAATTTTCTGTCAGTGCCTTATCTTATGAAGAAACAGAACCTCTCTAGCTAATGTGTGGTTTC
  TCCTTCCCTGCCCCCACCCCTAGGCTCACCTCTGCAGTCTTTTACCCCAGTTCTCCCATTTGAATACCA
  TACCTTGCTGGAAACAGTGTGTAAAATGACTGAAGTGATGATGCCCGAAGATGAAATAGATGCCAAATT
  AGATGGACATTGAAGCAACACTCAGCGTTGCCTAGCGTTAAAGGCACTGCAGAGAAATGAGGTGCAGAG
  GTGGCCCCTCTGAGTATTTATTTGACTCAGGTACCAGTGGTACATATATACAGTGTAATTATGACCAGG
  CTGGTAAAATTGGCTGCTCGCAAACAATCCCCTTTTTTCCTGGCAGTATTTGGAATTTATCATTTATTA
  ATAACTATACATTTTTAAAGGCAGAAGAAGAAAATCTATCTATCTATCTATCTATCTATCTATCTATCT
  ATCTATCTATCTATCTAAATGACCTGACAGAAGAAAACTGTTAAAAATGGATATTATTGGAGGGGATTT
  AAAACAGTGGGTGTGAATTATCATTCTGATGGAAAGAAAATAGCAAAACAATGTGTTACAAGTATTTGC
  TAATAAACAGTATACTGCCAGCTTCTAATTGCTTTTTGATGTATGAAAGGCTTATATAATTTTCTTTTC
  GTTGGGTGACTTTTGCCAGATGAGAGGAGGTGGCACAGTGGTGAGTGCAGGGCACAGTCCTAGCCTTCT
  GTGGGTATACTTTTGGAGTTGTGACTTGGCTGTGAGGGCAGAAGTTGAAGTTGGGATCACTGTGACTTT
  GCACATGGAAAAATGCAGATTGCAGGCATAATTCATCTCTGACATTAGAGAAAAAGCTGTTATAGCACA
  ATTTAAATTTTGAGAGTTTGCTGTGTTTTTTTTTCACATAAAAGAGGCTGATTATTCTTTTTAGTTTAA
  TTTTATATCCTGTAATTCTTTGGATGGTTCCAAGATTCAGAAAAAATTCAGTAAATGCACCCCGTAAAT
  TGCTACCCTTTCCTTTATTTTCATACTTAGATCTGCTGTACATTGTATATATATATAATTTTTAAAATG
  CAGAAAGAAAATAATTTCCCTAAATATAATTGCAAACTGATTTCTTTTACTTTTTTGTGTGTGGGGGTG
  GGAGCTGTATCTGAATAAGTGGCATTCAGATTAGGGTCTTGAAAAATAAACCCAGAATCTTTAAAAGAA
  GCAAATAAACTAATAGACGCTTATTTTCCAAAATTTAAATTTAAGCTAGAAATGTAAATATTCAATTAA
  TTTGTTAAAAGTACTTTTATAAAGTTAAAAAAAATCCAACCAAAATTTTAGAAAGTCAGGCTCTTTTAG
  AAAGAAAGCTACACCCATTTCCTCAAATAACTGTTCCGAAAATTTATATGGTGGAATGCGCCATGTATA
  AACTGTGAATTGTATTGACAAATAAAGTTTGTAATTAAAGTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001017425.3
• Summary: This gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 3776
• MW: 75.9