NAGA (NM_000262) Human 3' UTR Clone

CAT#: SC217145

3' UTR clone of N-acetylgalactosaminidase alpha- (NAGA) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NAGA
• Synonyms: D22S674; GALB
• ACCN: NM_000262
• Insert Size: 1975 bp
• Sequence Data:
  Insert Sequence

  >SC217145 3’UTR clone of NM_000262
  The sequence shown below is from the reference sequence of NM_000262. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATCAAGAACCTGGAGATGTCCCAGCAGTGAGGAGCTGGGACATGTGACAGGCTGTGGTGGCACCACTGA
  GCCTAGACCATGGAGCCTTGGCATGCCCAGGGCAAGTGGGGAGGTTCTCTGCTCCCCAGGCCTGCTCGG
  TGACTGACCCCATCATACCCAAAGTGCAATCTCACGGCCAGGTTCTATGCCCTGTCCAAGCGTAAACCC
  TCTTGGAAACTTCTTTTGGGGCAATTTTCCTGTGGCCTTCCTGGCCTCTACTTCCATGTGCGCAGCCCC
  ACAGACGTTGCTGAGCAACTCGCCAGCCTCCTGAGCTCCATGCCCATCAGGACTCTAGCCTCTGACCTT
  GCTGTTGACTCTGAAATCAGGATTTGGAAGTTTTCGAATTAGGAGTAGAGAGATCTGACCTCTTGCCAG
  GAATGCCCATGGATCATGTGATTGGCTTTTCTACCCATAGAGGGCCTTGCAGCCTGATACCACCTGGGA
  GTGAGGGTCACAAAGGAGACCTTGGCTCCCTCAGGTCACCAATAAACCTGTTCTTTAATCAAGTAGTTG
  TAATATGGACCTGGGGTACCTTTAGTTAGGGACTCTGGTGGCACTACACGGGGTGCTGGATTCAAATCT
  CACCTCTATGGATGATGTCTGGCATCGTCTGGCCCCTCCTTTTCCAAAGTGACCAGAAGATTGTACTTC
  CTAGAGATGCCAGACTTGCCTCACACCCAGGAGCTGCCCCTGCTGTGCCTGGTATGCTGGCAGTGGTGA
  TGGTGACAACACAATGAGGCCAAGTAGCAATGCTCCCAGGTCCTGCAGCTTGTGCTCTGCCCAGCAGTG
  CCCCTGGCCCAGCACCTCAGCCTGCTCTTCTCTCTGGTGGCTCCACCCCAACCCCTACTTCTTTTCCTT
  TATTTCTCTCCCAACCTGGCTGCAAAGGAGGTTCATATTCCCCAGATGTGTCCACAAACCTGTCCAGGA
  GTTGTGTCTTAAAATTGATTTCCTGTCCCTCTTCCTAAGGACAGGGGTAACAGTCAAAGTGAAGGAATG
  ATGGGTTCTTGCCTGTCTTGCTTTGGTCCGTATTTCCCAAGCCCTCCTCTTTTAGCCAAGGCTACAGGG
  CACTTGTCTTCCCTGGTGGCTCTCCCCTTTGGAGAGAGCTAGTGGCAGAGCAACCTAGCTAGGTCCTCC
  TTGGGCTTTGAATGCAGGAGCACCCAGTGCCCCAGGCCTTGCACTGTGCTGCCCAAGGAGTATTTTCTG
  GGTGGATCTCCCATACTCCTCAGGGTTCTGAGGTCCTTGTATTTAGGGTATGGTGTAAAAACTTACATA
  TGCACATTTTGTTACATCATTTAAGATGATTGAAGCTTTACTGGTTAAGTATAGGGTCACCAGAGAAAG
  TTCCTTTCCCCAGTTGATGTCTTGAGGCTGTTGAGAAACTTAGGACAGAAGCCTACCTCATCCCAGGCC
  TGCCCCTCCGTGCATAGCTCTGCCTTTGGGTCTTGTTCATAGTGCTGTTCAGGCAAAATGGAATAGTCT
  CAGGTGGGAACACCTTCTCTTGCTAGCTCCAGGAAGGCTTTGTGGGAAATGAGTTGAGACCATGGACTT
  GGGAGTTGGGGGCCAGAGTTGAGCCTGGACTTGACCAATTAATTCCCTTACCTTTCAGCCCAAGATAGC
  TACATGTCTTTACTTGCTGTATAAGTTTTCCTTTTGTCCTGGGGGTGCCTGATTGATCCTATCTCTTCA
  CCCTTCATTCTTTCAACAAACATGCCATCTATCTCCCAGGACATTTATTCCCTTCTATTCAAGGCCAGT
  TAGGAATGCAATTATTTTTTTTTTTCAGTTAAATACAGACTTGTTTGGACGCAAGGTACCCTTTCTCTT
  TTTAAAAACTTTTTATCATGAAAACTTTCAAATACAAAAATAAAATGGTAGAGTGAACCTCCATGTACC
  CAGCATCCAGCTCCAGTAACCATCAACGTGTACCATTATTTCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000262.3
• Summary: NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase, which cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease). [provided by RefSeq, Jul 2008]
• Locus ID: 4668
• MW: 73.2