CHRNA2 (NM_000742) Human 3' UTR Clone

CAT#: SC216875

3' UTR clone of cholinergic receptor nicotinic alpha 2 (neuronal) (CHRNA2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHRNA2
• ACCN: NM_000742
• Insert Size: 1903 bp
• Sequence Data:
  Insert Sequence

  >SC216875 3’UTR clone of NM_000742
  The sequence shown below is from the reference sequence of NM_000742. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTGCCTCCGTTCCTAGCTGGAATGATCTGACTGCACCTCCCTCGAGCTGGCTCCCAGGGCACCCCCGCT
  GACCATCTTTTGACCGTCTCTGCTACAGCTGCCTCTGGTGTCCCCTTTGGGATGAGCAGGTGCCTCTCC
  GCGGAGTCTGGACACTGACCCAGGGATTGCAGGCTTCCATCCAGCTTAACGTTATTGTACCAGGGCTGA
  CAGCCTGCTGGGTGCAAAGCCCTGTTTTGGATACGGAGGAGCTGGGGAAGCTCTGCAGAAGGTCGCAAT
  AGTGTGGTGACCAAGTGGGGCAGAGTAAGATTCTCCTGGAGGAGCAGTGCAGACCCTAAGTGACAGAAG
  GGTCAACCAGGGGGACAGTGGGGCCAGGGTGACGAGGAAGGGAATGCAGACCTCAAAGGAGGGGCCGTG
  GGGGCAAAGGGGAGGGTTCTTGGATGTGGAAGGGCTTTGAACAATGTTTAGATTTGGAGATGAGCCCAA
  AGTGCCAGGGAGAACAGCCAGGTGAGGTGGGAGGTTGGAGAGCCAGGTGAGGTCTCTGTAAGTCAGGCT
  GGGGTTGAAGTTTGGAGTCTGTCCGAGTTTGCAGGGTGCTGAGCTGTATGGTCCAGCAGGGGAGTAATA
  AGGGCTCTTCTGGAAGGGGAGGAAGCGGGAGGCAGGGCCTGCACCTGATGTGGAGGTACAGGGCAGATC
  TTCCCTACCGGGGAGGGATGGATGGTTGGATACAGGTGGCTGGGCTATTCCATCCATCTGGAAGCACAT
  TTGAGCCTCCAGGCTTCTCCTTGACGTCATTCCTCTCCTTCCTTGCTGCAAAATGGCTCTGCACCAGCC
  GGCCCCCAGGAGGTCTGGCAGAGCTGAGAGCCATGGCCTGCAGGGGCTCCATATGTCCCTACGCGTGCA
  GCAGGCAAACAAGAAAGACCATCCTGAGCTGCTCCTGACCCACCTCAAACTCATCTCATTTGGCCTGTC
  CTCCCTCCCTTCACCCCTCCTTCCCTCCCCAGGAACCCTGTTCCCTGTGCCTGGCTCCATCAGGAGGAG
  GCCTCACTTGGGAGTTGAAGATCTTTGCCTTTAGTTCCTTTGATTCTGAACTTCTCAAAGGCAGGTACT
  CTGCCTGTTATTTTTCCCACCCCCAGCACCTAGCACAGCGCCGGGTGAGTGGGGTCAGCACTTAGGGCG
  TTTGTTCTGAGTGGTTGAGGGGAGCAAGCTGGATTGTTTGGGCCCCAGGACTGTTGTGTAGATGGCAAA
  GCTGTGGTCCCGTCCCTTATCAGCCTGCCTCGTCTCTCCCAGAGCCCCGAGGTGTGTTGACCTGCTCTG
  AACCCTTCAGAGCAGCCTGTGTGTGCACCTTGGAATGCAATGCACCACCCCACAGTCTGAGGCTGCACC
  CAGGATCCCAGGCCGAGCTGCTGCTCCTGGGATCGGGCAACAGACCTCCCCCCAACTCTGTCCAGACTG
  GAGGGTGGGACATTGGCAGCTAACAGATGTGGGCATCGGGTTCTCGGGACAGCATGGAGGCTCCAGGCA
  GGAAGCCGAGCAGGGCGAGCTTCCAGTTTCCTTTTGGTCCTAGGGGTCCCCTCCTTCCAAACCCCAAAA
  TGTCTCTCCCTGACACTCTCAGGGGAAACCAGCCTGGCTGTGACGCCCTGGCCCAGGCCCTGCCATGCC
  TCACTTCTCTCCCACAACCCCCCTGCTCATGGCACATCTGGTCTCTGGCTCCTGTCTACTCGTGTGCAT
  GCAGCCCCCAGCGCCCCCTCTCCTCCCACTTGCTCTCCGGAGGCCTCAGAGCTCTCGGGTCCAGGCTCC
  CAGACCCACTCTCCTGAGACAGTGATGTTCACTTCTGTTATCCATGTCTCCCATCCATCTTGCATGTGA
  ATAAAGAGATTAAATAAAGCTGCACCTACTGCTGACTCAA
  AGCGGACCGACTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCC
  CAACCTGCCATCACGAGATTTCGATTCCACCGCCGC
  
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000742.4
• Summary: Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq, Nov 2009]
• Locus ID: 1135
• MW: 67