B3GALNT1 (NM_033169) Human 3' UTR Clone

CAT#: SC216849

3' UTR clone of beta-13-N-acetylgalactosaminyltransferase 1 (globoside blood group) (B3GALNT1) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: B3GALNT1
• Synonyms: B3GALT3; beta3Gal-T3; galT3; Gb4Cer; GLCT3; GLOB; P; P1
• ACCN: NM_033169
• Insert Size: 1906 bp
• Sequence Data:
  Insert Sequence

  >SC216849 3’UTR clone of NM_033169
  The sequence shown below is from the reference sequence of NM_033169. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ATGCTAAGGAACACCACATGCCATTATTAACTTCACATTCTACAAAAAGCCTAGAAGGACAGGATACTT
  TGTGGAAAGTGTTAAATAAAGTAGGTACTGTGGAAAATTCATGGGGAGGTCAGTGTGCTGGCTTACACT
  GAACTGAAACTCATGAAAAACCCAGACTGGAGACTGGAGGGTTACACTTGTGATTTATTAGTCAGGCCC
  TTCAAAGATGATATGTGGAGGAATTAAATATAAAGGAATTGGAGGTTTTTGCTAAAGAAATTAATAGGA
  CCAAACAATTTGGACATGTCATTCTGTAGACTAGAATTTCTTAAAAGGGTGTTACTGAGTTATAAGCTC
  ACTAGGCTGTAAAAACAAAACAATGTAGAGTTTTATTTATTGAACAATGTAGTCACTTGAAGGTTTTGT
  GTATATCTTATGTGGATTACCAATTTAAAAATATATGTAGTTCTGTGTCAAAAAACTTCTTCACTGAAG
  TTATACTGAACAAAATTTTACCTGTTTTTGGTCATTTATAAAGTACTTCAAGATGTTGCAGTATTTCAC
  AGTTATTATTATTTAAAATTACTTCAACTTTGTGTTTTTAAATGTTTTGACGATTTCAATACAAGATAA
  AAAGGATAGTGAATCATTCTTTACATGCAAACATTTTCCAGTTACTTAACTGATCAGTTTATTATTGAT
  ACATCACTCCATTAATGTAAAGTCATAGGTCATTATTGCATATCAGTAATCTCTTGGACTTTGTTAAAT
  ATTTTACTGTGGTAATATAGAGAAGAATTAAAGCAAGAAAATCTGAAGTATTGTCTTGTTTTTAAAAAA
  TACAGTTCCTAGTGTTTTTAGAAGTCACTTAATTTGTCTCATTTTTCCACCTGGAAATTAGGAATAATG
  TAGAATGCAAGGCAGTAATTTCCTTTTGGAAAGGACTCTGAAGGCAGAAAAGAAGGGAGAGAACCTCAT
  GGGCAGAATATTATAAAAAGAGTGTCATATTCCAGCATTTGAATTGGAAAGAGAAGAGTGAAGATCCAA
  GTTGCATTATTAATCTGCCCTGTGTTTTTTCCTTTTAACAATCAGTTTGAGCTGCTGCTGTTATGAGTT
  TCTCATCAAGATGAAAGCCCTAATATGTAAAGTCAAATCCGATTTAAATTTTGTGCTTTTATAGAAAGA
  AATTTCTTCATAGACGTGGTGATATATCATTTGTTGGACCTGCTAATAGTAGGTCAAAGGGGAGCACTC
  CTTGCCCCCTGTTCCTGGGTTTATGCAGTTTTCTTTTTAGAGTTTATATAGGGCAAGTGGTTCTTTTTC
  TCTGAATTACAGGATGGAAAAAGGTCATATCCTTTGTCAGGAAATATAAACTTGAAAGTATGTAGTCAG
  CTCTTGTAATACTCATATTTATGATTGTCCTATATGAAAAACAACTTCAGTTAAAACTATAATGTGTGA
  TTCTGTATAACAAGGTGATGTCTGTTTCCCAGGGCTCAGACCTAATCCAGTTATAATAAAATCAATTAA
  ATGAAATATTCTATAGAATCGATCTATGCCCTTGTTAATCTCCATCCATATAGGAGTCACGTTCTTTAA
  GACAGATGGTGGTAGTTATTTTTGTGGCATGGTTAGATTTGACTGGTTTTGCAGAAGATTACAGTTATG
  TACTGCATAATGACATATACAATAGTGGTCCCATAAAATTATAATGGAGCAGAAAATCTATTGCCTCAT
  GATGTTTTAGCCGTTTTAATGTCATAGCCTAGTGCATTACTCACGTGTCTGTGGAGATGCTGGTGTAAA
  CAAACCTACTACACTGCCAGTTGTATAAAAGTACAGCACATTCAGTTATGTACAGTATGTAATACTGAT
  AATGACAATAAATGACACCGGTTTGTGTATTTACTTTTTATAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_033169.3
• Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). The encoded protein of this gene does not use N-acetylglucosamine as an acceptor sugar at all. [provided by RefSeq, Mar 2017]
• Locus ID: 8706
• MW: 75.5