Versican (VCAN) (NM_001126336) Human 3' UTR Clone

CAT#: SC216815

3' UTR clone of versican (VCAN) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: VCAN
• Synonyms: CSPG2; ERVR; GHAP; PG-M; WGN; WGN1
• ACCN: NM_001126336
• Insert Size: 1898 bp
• Sequence Data:
  Insert Sequence

  >SC216815 3’UTR clone of NM_001126336
  The sequence shown below is from the reference sequence of NM_001126336. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGCCGGAGGTGGCAGGAGTCGAGGCGCTGATCCCTAAAATGGCGAACATGTGTTTTCATCATTTCAGCC
  AAAGTCCTAACTTCCTGTGCCTTTCCTATCACCTCGAGAAGTAATTATCAGTTGGTTTGGATTTTTGGA
  CCACCGTTCAGTCATTTTGGGTTGCCGTGCTCCCAAAACATTTTAAATGAAAGTATTGGCATTCAAAAA
  GACAGCAGACAAAATGAAAGAAAATGAGAGCAGAAAGTAAGCATTTCCAGCCTATCTAATTTCTTTAGT
  TTTCTATTTGCCTCCAGTGCAGTCCATTTCCTAATGTATACCAGCCTACTGTACTATTTAAAATGCTCA
  ATTTCAGCACCGATGGCCATGTAAATAAGATGATTTAATGTTGATTTTAATCCTGTATATAAAATAAAA
  AGTCACAATGAGTTTGGGCATATTTAATGATGATTATGGAGCCTTAGAGGTCTTTAATCATTGGTTCGG
  CTGCTTTTATGTAGTTTAGGCTGGAAATGGTTTCACTTGCTCTTTGACTGTCAGCAAGACTGAAGATGG
  CTTTTCCTGGACAGCTAGAAAACACAAAATCTTGTAGGTCATTGCACCTATCTCAGCCATAGGTGCAGT
  TTGCTTCTACATGATGCTAAAGGCTGCGAATGGGATCCTGATGGAACTAAGGACTCCAATGTCGAACTC
  TTCTTTGCTGCATTCCTTTTTCTTCACTTACAAGAAAGGCCTGAATGGAGGACTTTTCTGTAACCAGGA
  ACATTTTTTAGGGGTCAAAGTGCTAATAATTAACTCAACCAGGTCTACTTTTTAATGGCTTTCATAACA
  CTAACTCATAAGGTTACCGATCAATGCATTTCATACGGATATAGACCTAGGGCTCTGGAGGGTGGGGGA
  TTGTTAAAACACATGCAAAAAAAAAAAAAAAAAAAAAAAAAGAAATTTTGTATATATAACCATTTTAAT
  CTTTTATAAAGTTTTGAATGTTCATGTATGAATGCTGCAGCTGTGAAGCATACATAAATAAATGAAGTA
  AGCCATACTGATTTAATTTATTGGATGTTATTTTCCCTAAGACCTGAAAATGAACATAGTATGCTAGTT
  ATTTTTCAGTGTTAGCCTTTTACTTTCCTCACACAATTTGGAATCATATAATATAGGTACTTTGTCCCT
  GATTAAATAATGTGACGGATAGAATGCATCAAGTGTTTATTATGAAAAGAGTGGAAAAGTATATAGCTT
  TTAGCAAAAGGTGTTTGCCCATTCTAAGAAATGAGCGAATATATAGAAATAGTGTGGGCATTTCTTCCT
  GTTAGGTGGAGTGTATGTGTTGACATTTCTCCCCATCTCTTCCCACTCTGTTTTCTCCCCATTATTTGA
  ATAAAGTGACTGCTGAAGATGACTTTGAATCCTTATCCACTTAATTTAATGTTTAAAGAAAAACCTGTA
  ATGGAAAGTAAGACTCCTTCCCTAATTTCAGTTTAGAGCAACTTGAAGAAGAGTAGACAAAAAATAAAA
  TGCACATAGAAAAAGAGAAAAAGGGCACAAAGGGATTGGCCCAATATTGATTCTTTTTTTATAAAACCT
  CCTTTGGCTTAGAAGGAATGACTCTAGCTACAATAATACACAGTATGTTTAAGCAGGTTCCCTTGGTTG
  TTGCATTAAATGTAATCCACCTTTAGGTATTTTAGAGCACAGAACAACACTGTGTTGATCTAGTAGGTT
  TCTATTTTTCCTTTCTCTTTACAATGCACATAATACTTTCCTGTATTTATATCATAACGTGTATAGTGT
  AAAATGTGAATGACTTTTTTTGTGAATGAAAATCTAAAATCTTTGTAACTTTTTATATCTGCTTTTGTT
  TCACCAAAGAAACCTAAAATCCTTCTTTTACTACA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001126336.3
• Summary: This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
• Locus ID: 1462
• MW: 74.3